Kaswer Musa Jaffar AL_Taraihi

ABSTRACT Globally, colorectal cancer represents the most common cancer. Its incidence was increases with advancement of age. Both men and women face a lifetime risk of 6% to get colorectal cancer. Colorectal cancer represents a major cancer-related health problem in Iraq and significant attribution to morbidity and mortality related to cancer. It ranked as the 7th most common malignant tumor in Iraq and account for about 5.4% of all cancer in this country. Inflammatory bowel disease represent a risk factor for development of colorectal carcinoma and this was related to increased severity and duration of the disease. On other hand colorectal polyp may transform to carcinoma and this risk was more with larger polyps and polyps with sever dysplasia. Adenomatous Polyposis coli gene is a tumor suppressor gene. Its inactivation represents a common genetic alteration in this tumor. This mutation in such gene occur both I sporadic as well as inherited cancers. This study was designed to clarify the role of APC gene and its protein in both colorectal cancer and its precancerous lesion and put the shadow on its possible role in disease development and progression. APC protein was reported in 33 out of 60 cases of colorectal cancer, 16 out of 50 cases of colorectal polyps, and 11 out of 50 cases taken from patients with ulcerative colitis. There was no expression of APC protein in normal looking tissues in all cases. APC expression was showed significant differences when grade and stage were considered. APC gene was altered in colorectal cancer and its precursor lesion and this alteration were correlated well with grade and stage of disease. The alteration plays a role in both disease development and progression

ABSTRACT The aim of the present is to determine the immunohistochemical expression of EGFR, EMA and CD117 in case of urinary bladder transitional cell carcinoma and their relation with staging of tumor and its impact on further prognosis. EGFR immunohistochemical study showing (61.5 %) positive in case of urinary bladder carcinoma with significant difference between staging of tumor (p value 0.019) with signification expression between low grade and high grade tumor with P value 0.002 (6.2%) positivity in high grade versus (15.4 %) positivity in low grade). EMA showing strong positivity in urothelial carcinoma 96.2% without significant difference between staging and grading of tumor with p valve of (0.57) and (0.227) respectively CD117 immunohistochemical study showing total positive cases of urinary bladder carcinoma of 69.2% with significant difference in comparison with staging and grading of tumor with (P-value=0.00) for each respectively. Our result demonstrate that EGFR and CD 117 immunohistochemical staining showing strong positivity in majority of urothelial carcinoma cases with significant relation to both pathological grading and staging of tumor. Application of both markers may play a role to give idea about behavior of tumor and possibility of further recurrence with and even direct therapy application EMA showing strong positive expression which give idea about tumor origin from epithelial lining. It had no benefit in assessment of tumor grading and staging

ABSTRACT Diabetes mellitus is a common endocrine disease. It impairs glucose homeostasis.Rosiglitazone (RSG) is an efficient hypoglycemic remedy.It enhances insulin sensitivity. There a conflict regarding safety of this drug. This work was designed to solve this conflict.Fasting blood sugar were significantly dropped down in all studied groups as compared to diabetic group.All groups showed significant decrement in serum level of insulin as compared to normal control. There was significant increment in the level of serum urea level in diabetic rats. This level was significantly decrease in those diabetic rats treated with RGS in a dose of 0.1 & 0.2 mg. Histological examination of the pancreas from diabetic rat’sshowing necrosis of both exocrine and endocrine pasts with lymphocytic infiltrations and vascular congestion,this in comparison with essentially normal histolologic appearance of the pancreas in all other groups. The study highlights the effectiveness of RGS in controlling blood sugar as well its protective effect on pancreas.

Abstract Background: Lung cancer is a major public health problem. It is the most common cause of cancer-related death in men and the second most common in women. Moreover, EML4-ALK( the echinoderm microtubule￾associated protein like4-anaplastic lymphoma kinase fusion gene ) has been identified as a potent oncogenic driver in non-small-cell lung carcinoma (NSCLC), in particular adenocarcinoma (ADC) and less commonly squamous cell carcinoma. The aim of current of study was to investigate ALK protein overexpression and its correlation with EML4- ALK gene rearrangement by CISH. Method: The paraffin embedded blocks were retrospectively collected from 80 patients diagnosed with NSCLC. ALK protein study was performed by using Monoclonal Mouse Anti-Human for IHC and for EML4-ALK fusion gene by using CISH (CISH working solution /Zytovision, Germany). Results: ALK protein overexpression was detected in (10%, n= 8/80) of NSCLC and the ALK gene rearrangement was (7.5%, n= 6/80). There was high concordance in the assessment of ALK gene rearrangement by CISH techniques and ALK protein expression by IHC ( P= 0, r= 0.94). Conclusion: IHC and CISH were very well correlated in detection of ALK protein expression and ALK gene rearrangement, respectively. Also, CISH is a new and highly practical method to detect EML4- ALK, therefore, it could be introduced in plans of ADC management and less commonly SCC of the lung.

Furat Al-Awsat hospital, Iraq Abstract Aim: Present study designed to evaluated immunohistochemical expression of both p16 INK4a and HPV (Human papilloma virus). Different cervical lesion (Benning, Premalignant and malignant) and its relation one to other in development of cervical carcinoma. Materials and Methods: forty-nine cases of cervical lesion formalin fixed - paraffin embedded tissue specimens of the cases. Stained tissue sections of all the cases were evaluated to confirm diagnosis and assure the presence of representative tissue material. All cases with tissue sections that were appropriate for immunohistochemical evaluation, positive tissue controls were obtained according to primary antibodies manufacturer’s data-sheets tissue sections of a uterus adenosequamus as p16 primary antibody, sections of skin wart tissue were used as positive control for HPV marker. A statistical analysis was preformed between the immunohistochemistry for marker each other. Results: The test results for control group: All patients had with histopathology changes and staining of cytoplasm. Among Benign group; twenty samples (100%) showed benign lesion change. Among premalignant group seven samples (78%) showed CIN-1 change. two samples (22%) showed CIN-2. Among Malignant group; six samples (30%) showed poorly differentiated. Eleven samples (55%) showed moderate differentiated. three samples (15%) showed well differentiated. Also, the intensity of premalignant lesion shows between weak and moderate and no one showing in strong, while for malignant intensity showing two sample in weak and two sample in moderate and two strong intensity.

ABSTRACT Submitted: 28.01.2020 The aim of the present is to determine the immunohistochemical expression of EGFR, EMA and CD117 in case of urinary bladder transitional cell carcinoma and their relation with staging of tumor and its impact on further prognosis. EGFR immunohistochemical study showing (61.5 %) positive in case of urinary bladder carcinoma with significant difference between staging of tumor (p value 0.019) with signification expression between low grade and high grade tumor with P value 0.002 (6.2%) positivity in high grade versus (15.4 %) positivity in low grade). EMA showing strong positivity in urothelial carcinoma 96.2% without significant difference between staging and grading of tumor with p valve of (0.57) and (0.227) respectively CD117 immunohistochemical study showing total positive cases of urinary bladder carcinoma of 69.2% with significant difference in comparison with staging and grading of tumor with (P-value=0.00) for each