جنان محمد حسين فيروز علي

Abstract This study aimed to explore the dissemination of blaOXA-23 gene among bacterial causative agents of urinary tract infections. A total of 54 samples of urine from patients with Urinary Tract Infection (UTI) during the period from November, 2021 to March, 2022, out of 36 samples were positive growth. Gram-negative bacteria were 27 (75%) (Which include 11(31%) of isolates were identified as E. coli, 7(19%) of isolates identified as K. pneumoniae, 6(17%) Acinitobacter bumanni, 2(8%) proteus merabilis, and 1(3%) Pseudomonas aeruginosa.). While 9 (25%) were gram-positive isolates 6 (17%) were S. aureus and 3(5%) were Enterococcus faecalis. Carbapenems susceptibility was performed by disk diffusion method, where Meropenem antibiotic showed that out of 36 isolates 19 (55%) were resistant (6 (31.5%) A. bumanni, 3 (15.8%) S. aureus, 3 (15.8%) E. feacalis, 2 (10.5%) E. coli, 2 (10.5%) K .pneumoniae, 2 (10.5%) P. mirabilis and 1 (5.4%) P. aeruginosa), as for Imipenem antibiotic showed that out of 36 isolates 16 (45%) were resistant (6 (37.5) A. bumanni, 3 (18.7%) K. pneumonia, 3 (18.7%) S. aureus, 2 (12.5%) E. coli, 1 (6.3%) P. merablis and 1 (6.3%) P. aeruginosa.). Total DNA extract samples were used in blaOXA-23 gene detection, results showed that total Acinetobacter baumannii isolates(6) were positive for blaOXA-23 gene, then in K. pneumoniae out of 7 isolates 4 were positive for blaOXA-23 gene, out of 11 E. coli isolates 2 were positive for blaOXA-23 gene, in Proteus mirabilis and pseudomonas auroginosa isolates 2 and 1 respectively all were positive for blaOXA-23 gene, while in all isolates of S. aureus and Enterococcus faecalis blaOXA-23 gene was not detected. We conclude that various types of gram negative UTI causative agents harbored blaOXA-23, meanwhile its absence in isolated gram positive isolates.

Abstract Lactobacillus is one of the most important genera in the human and animal digestive tract. They can be found in dairy and non-dairy probiotic foods, and they aid in the restoration of intestinal microflora, which is good for one's health. Lactobacillus strains were phenotypically identified and defined using biochemical characteristics. For the current study, 120 fecal samples were collected from healthy breast-feeding infants aged two years and under. We looked at a couple of primers on complex communities to see if they could service our investigation of complicated micro-ecosystems. The two primers provided positive results with L.acidophilus and L. helveticus. Their strains came from the feces of breast-fed litters. The remains obtained from DNA samples were sequenced and uploaded to the GenBank "NCBI" database after PCR augmentation.

Abstract: This study of urinary tract infection has been planned to isolation and identification of bacterial pathogens. The urine were collected from 516 patients for Al-Najaf Hospitals, they were including (81) male and (435) female. Patients aged between 5to60 years. Urine culture had been done for all the 516 patients who were included in this study. Only 221 patients had positive urine culture and 295 negative urine cultures. The most common organism was Escherichia coli which was isolated from 200 patients with percentage of 38.75%. E.coli was the most prevalent followed by Staphylococcus saprophyticus 20.54% (106), Staphylococcus aureus 16.66% (86), Klebsiella spp. 13.17% (68), Streptococcus spp. 5.62% (29), Proteus spp. 3.48% (18) and Pseudomonas aeruginosa 1.74% (9). The antibiotic susceptibility test was done for E.coli isolates to 10 antibiotics.

Abstract The study involved the isolation of enterococcus bacteria and diagnosis of different types of diseases, where (190) samples of cows suffering from different diseases were obtain (34) (18.1%) isolation of Enterococcus were divided into 21 (15.6% ) Of the blood, 8 (52.4%) and 3 (17.6%) of the vagina, but not isolated from the spinal cord samples. The study was conducted on 2 isolates from the blood samples to become the number of isolates (36 isolates). And the study showed that type E. Faecalis was the most common species, with 75% followed by E. faecium (20.2%) and E.galinarium (4.8%), which also revealed the virulence factors of these bacteria, which play an important role in their disease. Bacteria have the ability to produce Haemolysin, Protease, Lipase, β-Lactamase, and the formation of capsules and their ability of Haemagglutination and of the effect of mannos on this process.

Abstract: Bacterial infections have been identified as one of the causes of infertility, bacteria can cause immobilization and agglutination of spermatozoa. The aim of research was to detect the bacterial pathogens in seminal fluid culture from infertile men and determine the antibiotic sensitivity pattern in vitro.Seminal fluid samples were collected for culture and antibiotic sensitivity testing and were processed according to the standard techniques of microbiology. A total of 120 seminal fluid samples collected from human males admitted to the infertility centers in Najaf province, 74 (61.66%) of samples revealed positive significant growth of bacteria, while 46(38.33%) samples were with no growth. Eleven species of different bacterial organisms were isolated.

The present study aimed to investigation the relationship between blood groups and viral hepatitis C in both sexes. Sixty blood samples were taken from patients who visited the Al-Sadr Educational hospital. The viral hepatitis was tested for these samples in the hospital laboratory. The blood samples were tested for the same samples. The results of the present study showed that the highest rate of infection with this virus was in people who carry the blood group O, which amounted to 40%, as the results showed when the distribution of patients in the age groups that the age group(1-20) years have the highest rate of infection which amounted (1.5),and the lowest rate of infection in the age group(41-60) years, when it reached (1) as for the distribution of patients for sex, there were no significant between them.

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