مواقع التدريسيينجامعة الكوفة
الدكتورة هدى جميل باقر الخلخالي
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الدكتورة هدى جميل باقر الخلخالي

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الملف الشخصي

الاسم:هدى جميل باقرياسين الخلخالي
الشهادة:دكتوراه
الاختصاص العام:الاحياء المجهرية
الختصاص الد قيق:فايروسات
الحالة الاجتماعية : متزوجة
الكلية:العلوم
القسم:علوم الحياة
البريد الالكتروني:[email protected]
حاصلة على شهادة البكالوريوس من قسم علوم الحياة / كلية العلوم / جامعة الكوفة بتاريخ 9/7/2000والماجستيرفي أ ختصاص الآحياء المجهرية .من قسم علوم الحياة / كلية العلوم / جامعة الكوفة . ولقب مدرس مساعد بتاريخ 4/10/2005بموجب الآمر الجامعي6082ولقب مدرس بتاريخ 31 /5 /2012 آستنادآ لللامر الجامعي ت.ق/ذي العدد17097.وحاصلة على شهادة الدكتوراه في الاحياء المجهرية /الفايروسات بتاريخ 4/3/2015.
الاحالاهتمامات البحثية

دراسة الفايروسات الطبية.
دراسة ألتهاب الكبد ألفايروسي نوع B
دراسة فايرس CYTOMEGALOVIRUS
دراسة في بعض أنواع الخلايا ألمناعية والتأثيرات الحاصلة فيها بسب الاصابة الفايروسية
الآهتمامات التدريسية
فايروسات
مناعة فايروسات
مناعة
الدورات المشتركة بها:
1- المشاركة في الدورة التدريبية لتعليم الحاسوب في نظام التشغيل( (Windows وتطبيقات ( (word,Internet,Power pointبحسب مناهج الرخصة الدولية لقيادة الحاسوب((ICDL.في مركز الحاسبة في رئاسة جامعة الكوفة في5/4/2009 ولغاية 23/4/2009
2-المشاركة في دورة طرائق التدريس التي اقامها مركز تطوير التدريس والتدريب الجامعي في جامعة الكوفة للفترة من25 /5/2008 ولغاية 15/6/2006.
3-الشهادة الدولية للحاسب والانترنت IC3 (Internet&Computing Cor Certification )
والتي تضمنت ثلاث اختبارات
أ-اختبار مبادئ الحاسب الآلي Computing Fundamentals
ب-اختبار تطبيقات الحاسب الآلي Key Applications
ج-اختبار تطبيقات الانترنت Living On-Line
3-المشاركة في الدورة التي اقامتها كلية الطب / جامعة الكوفة عن تقنية RT-PCR.

الاهتمامات البحثية

4

البحوث المنشورة

9
2022

Association of Parvovirus B19 Infection and increased Some immunological markers among soontaneous abortion Women

الباحثونMohammed K. K. AL-Koufi، Huda Jameel Baker AL-Khilkhali
المجلةHIV Nursing
مختصر البحث

The purpose of this study was to see if parvovirus (B19) was present in abortion women and if certain cytokines were involved in abortion induction. between August 2021 and January 2022 (2022). A total of 60 women who had previously had abortions were included in the study, as well as 25 healthy pregnant women who showed no evidence of chronic inflammatory disease, were gathered from Al Rifai hospital in Dhi Qar city. The patients range in age from 19 to 50 years. Parvovirus was examined on ELISA in blood samples taken from abortion women and healthy controls. A total of 60 samples were taken, with 17(28.3percent) positive results for parvovirus B19, And 43(71.6%) were negative for parvovirus while pregnant women's samples yielded nil (zero per cent) negative results for every 25 samples, using the ELISA test. The distribution of aborted women according to their abortion frequency was revealed. The first abortion was the highest percentage (34/60). the results appeared as follows at first 8(13.3%), second and third trimesters 7(11.6%) 2(3.3)respectively.Interleukin-2 levels were measured using an ELISA test aborted +parvovirus women show a significant (p=0.0483) were (54.66 ±29.97) compared to pregnant women in the control group (2.312±0.6295).the results revealed there are no significant differences( p=0.1207) in the levels ofC3 (131.0±6.049)in Positive parvovirus B19 were found in when compared to the control group(118.4±5.107). As for the results of complete blood count, they were as follows, lower significant white blood cells levels ( 3.534±0.0776 ) compared to the control group were (7.412±0.4661 ), and lower significant lymphocytes levels(1.128±0.1103) compared to control group were (2.816±0.2416), as well as lower significant haemoglobin (Hb) levels (8.941±0.5584) compared to the control group(11.68±0.1695), on the other hand, the results related to the neutrophil showed that there are no significant differences between the patients and the control group(4.111±0.5345)( 3.224±0.2375)respectively, also PLT, results showed lower significant levels(163.7±22.55) in +Parvovirus aborted women compared to control group(273.6±14.59)

2020

Identiϑication of HIV virus in Najaf city, Iraq

الباحثونThualfakar Hayder Hasan*، Raad A. Al-Harmoosh1, Huda Jameel Baker Al-khilkhali2
المجلةINTERNATIONAL JOURNAL OF RESEARCH IN PHARMACEUTICAL SCIENCES
مختصر البحث

Acquired Immune Deϑiciency Syndrome (ADIS) is a disease of the human immune system that results in a decline in the efϑiciency of the human immune system step by step to leave people exposed to many infections and tumours. It caused by the Human Immunodeϑiciency Virus (HIV). The ϑirst appeared of HIV in West Central Africa in the late 19th or early 20th century. The direct contact from personal mucus membrane or bloodstream and physical ϑluid (blood, vaginal semen ϑluid and breastfeeding milk) containing the virus is the unique viral transmission route. Out of 80 blood samples were taken from different areas of Najaf city, Iraq, for ages from 20 to 60 years (males and females) to the period from 1/1/2019 to 19/12/2019. The surface antigen of the HIV was detected by the ELISA technique and mini VIDAS by a virus-speciϑic kit. Out of 80 different patients by physical examination infected with ADIS: HIV viruses were the most incidences with 12 isolates (15%) while, there were 66 isolates (82.5%) were belonged to other infections and two strains (2.5%) were negative to any viral infection

2019

Using Rapid and ELISA Test for Detection Hepatitis C Virus and Human Immunodeficiency Virus among Healthy and Renal Failure Population

الباحثونHuda J.B. Al-Khilkhali، Ebtehal E. Shubba
المجلةJournal of Global Pharma Technology
مختصر البحث

Human immunodeficiency virus (HIV)-infected, hepatitis c virus (hcv)-uninfected sufferers are at risk for incident hcv infection, however little is understood approximately screening practices for incident hcv among HIVinfected individuals in HIV primary care clinics. The current study aimed at detecting the prevalence of HCV and human immunodeficiency virus (HIV) infection among haemodialysis patients that suffering from chronic kidney disease and healthy people using the Rapid Immunochromatographic Test (ICT) and enzyme linked immunosorbent assay (ELISA) In In the detection of these viruses .This study was carried out during the period from the November (2017) to January (2018), , a total of (28) patient on HD was collected from Specialized Center for Diseases and Kidney Transplant in AL-Sadder Medical City in AL- Najaf Governorate, and(30) samples Represented healthy people collected from the University of Kufa Faculty of Science.All samples ranged in age (20- 45) years. There were 35 male and 23 female. Viral detection revealed that there were 11(19%) was positive and 47(81%) was negative in Rapid (ICT). We further tested samples with ELISA for confirmed results Similarly 58 samples were tested for HCV out of which 16(28%) were ELISA positive and 42(72%) were negative for anti- HCV antibody but the age group(20- 28)year revealed high significant(p<0.05) in the ratio of infection with hepatitis C virus 71% (5:7) than other age groups. the results of this study showed high sensitivity and specificity for rapid (ICT) in present study represent good result for depend rapid test for detection of hcv antibodies and first-line tests in screening and prognosis of hcv infection..in all groups of patient's seropositive anti-HCV antibodies and seronegative anti-HCV tested for anti-HIV Ab typ1 and typ2 by Rapid (ICT) were negative (0.00%) in all age groups in both sexes. The current study showed that males are more affected with HCV (56%) than females (44%) and the prevalence of hcv contamination amongst kidney failure patients in this study was 57%. Results of the current study showed that the healthy groups were negative anti-HCV Ab by both methods. Keywords: Hepatitis C virus, Chronic kidney disease, Hemodialysis patients, ELISA, Rapid (ICT)

2019

Using Rapid and ELISA Test for Detection Hepatitis C Virus and Human Immunodeficiency Virus among Healthy and Renal Failure Population

الباحثونHuda J.B. Al-Khilkhali، Ebtehal E. Shubbar, Maysam M. Amjed, Noor S. Mohsen , Zahra Y. Abed Ziad
المجلةJournal of Global Pharma Technology
مختصر البحث

Human immunodeficiency virus (HIV)-infected, hepatitis c virus (hcv)-uninfected sufferers are at risk for incident hcv infection, however little is understood approximately screening practices for incident hcv among HIVinfected individuals in HIV primary care clinics. The current study aimed at detecting the prevalence of HCV and human immunodeficiency virus (HIV) infection among haemodialysis patients that suffering from chronic kidney disease and healthy people using the Rapid Immunochromatographic Test (ICT) and enzyme linked immunosorbent assay (ELISA) In In the detection of these viruses .This study was carried out during the period from the November (2017) to January (2018), , a total of (28) patient on HD was collected from Specialized Center for Diseases and Kidney Transplant in AL-Sadder Medical City in AL- Najaf Governorate, and(30) samples Represented healthy people collected from the University of Kufa Faculty of Science.All samples ranged in age (20- 45) years. There were 35 male and 23 female. Viral detection revealed that there were 11(19%) was positive and 47(81%) was negative in Rapid (ICT). We further tested samples with ELISA for confirmed results Similarly 58 samples were tested for HCV out of which 16(28%) were ELISA positive and 42(72%) were negative for anti- HCV antibody but the age group(20- 28)year revealed high significant(p<0.05) in the ratio of infection with hepatitis C virus 71% (5:7) than other age groups. the results of this study showed high sensitivity and specificity for rapid (ICT) in present study represent good result for depend rapid test for detection of hcv antibodies and first-line tests in screening and prognosis of hcv infection..in all groups of patient's seropositive anti-HCV antibodies and seronegative anti-HCV tested for anti-HIV Ab typ1 and typ2 by Rapid (ICT) were negative (0.00%) in all age groups in both sexes. The current study showed that males are more affected with HCV (56%) than females (44%) and the prevalence of hcv contamination amongst kidney failure patients in this study was 57%. Results of the current study showed that the healthy groups were negative anti-HCV Ab by both methods

2019

Prevalence and Some Epidemiological Symptoms of Molluscum contagiosum Virus Infection in Al-Najaf Province

الباحثونEbtehal Edrees Shubbar1، Huda Jameel AL-Khilkhali1, Nawras Alwan AL-Barqawi1
المجلةIndian Journal of Public Health Research & Development,
مختصر البحث

Molluscum contagiosum virus causes awidely spread infection worldwide. Which can affect imunocompetent as well as immunocompromised individuals. Corresponding native studies are still rare. Current study targeted the screening of the mcv infections among patients visited Al-Sadr Teaching Hospital in Al Najaf Province. During the study period a total of 619 patients were clinically diagnosed with the virus from 3699 ptients who visited the dermatology clinic, Furthermore histological study was carried out to investigate the age effect on developing the infection. patients with MCV comprisd 349 males, and 270 females. According to age patients were divided into six age groups, the 0-5 years age group occupied the first order with 173 patients. Regarding the month of the study, April was the first recording 110 patients. Regarding the histological study the higher the age was the more the inclusion bodies to be noticed. Molluscum contagiosum virus can infect various age groups of both genders. Different samples taken from different ages showed common features of MCV cytopathic effects but with different density of their numbers in each sample.

2018

THE ROLE OF HEPATITIS C VIRUS INFECTION IN PATIENTS WITH CHRONIC KIDNEY DISEASE IN NAJAF GOVERNORATE, IRAQ

الباحثونHuda J. B. AL-Khilkhali، لا يوجد
المجلةBiochem. Cell. Arch.
مختصر البحث

ABSTRACT : Hepatitis C virus (HCV) infection is a significant is frequency problem found among patients of hemodialysis (HD). The current study aimed at detecting the prevalence of HCV infection among haemodialysis patients that suffering from chronic kidney disease besides assessment the role of virusin increasing the concentration of some important parameters that indicate renal failure including creatinine, urea, albumin,and total serum protein and glucose level in the blood of patient.This study was carried out during the period from May to September (2017), a total of (45) patient on HD was collectedfromSpecialized Center for Diseases and Kidney Transplant in AL-Sadder Medical City in AlNajaf Governorate. 26 male and 19 female with age ranging from 22 – 61 years. Viral detection revealed that there were 19(42%)were positive for HCV on Rapid Immunochromatographic Test (ICT). While the result fromenzyme linked immunosorbent assay (ELISA) technique showed that24(58%) werepositive for anti-HCV antibody but the age group (48- 60) year revealed high significant (p<0.05) in the serum concentration than other age groups. The results of this study showed increase in the level of creatinin in all patients with renal failure, especially those with HCV infection in age group (35-47) year and the results of serum urea level revealed a highly significant (P<0.05) elevation in the serum of all patients had serum urea level above 150mg/dl and especially in age group (22-34 and 35-47) years among patients were positive anti-HCV antibodies. The results were explained to the rest of the parameters increase in albumin and total serum protein levels in all groups of patient’s seropositive anti-HCV antibodies in comparison to negative antiHCV antibodies patient’s. As for the level of glucose were a significant reduction (p<0.05) in the means of glucose level in all age groups of patients seropositive anti-HCV antibodies compared with negative anti-HCV antibodies haemodialysis patients. Key words : Hepatitis virus, chronic kidney disease, hemodialysis patients, ELISA, rapid (ICT).

2017

Determination of some Hematological Parameters in Hepatitis B Patients in Najaf City

الباحثونHuda J.B. AL- Khilkhali، مفرد
المجلةمجلة جامعة بابل
مختصر البحث

Abstract This study was carried out on 50 patients, who were admitted to Al-Hakeem hospital (Center Laboratory) in AL- Najaf governorate during the period from January to March (2015), their age ranged between (12-65) years. Fifteen healthy individuals without any evidence of chronic inflammatory disease depended as control. Subjects of this study were investigated importance of measuring the blood parameters in hepatitis B patients, and detection of the patient's immune response against viral infection.The obtained results showed that 50 were seropositive hepatitis B in ELISA technique. , the age groups (32-41) and (42-51) years showed a highly significant (P<0.05) elevation in patients than other patients. In contrast white blood cell count indicate that the age groups (12 – 21) and (42 – 51) years recorded high significant difference (p<0.05) compared with healthy groups and other patients while Lymphocyte and monocyte percentage increase in in more groups of patients suffering from hepatitis B compared with healthy control group specific in age groups (42 – 51) and (52 – 61) years in comparison to other patients. In the other hand the result of granulocytes, the basophilis showing high – significant (p<0.05) increase in patients group as compared to healthy control group and other granulocytes. Keyword: Hepatitis B, Hematological Parameters, Differential leukocytes Count

2016

Detection of Autoimmune Hepatitis among Chronic Cases of Hepatitis B virus in AL-Najaf province

الباحثون Mohammed A. K. AL-Saadi ، Huda Jameel AL-Khilkhaly
المجلةمجلة علوم الحياة
مختصر البحث

This study was amid to investigate the existence of HBV and the diagnosis of autoimmune hepatitis (AIH) in addition study (C3, C4) Levels among Chronic Hepatitis B Patients. The current study was conducted on Seventy-Six samples, formed (55 males and 21 females) with age ranging (11-72) year were collected from Center Health Laboratory/Al-Hakeem Hospital, and AL-Sadder medical city in AL-Najaf city, during the period from January (2013) to August (2013). Fifteen healthy individuals without any evidence of chronic inflammatory disease involved as control, age ranging (21-50) years. The results showed that 76 HBsAg seropositive in all age groups but the age group (44-54) year revealed high significant(p<0.05) than other age groups. While 35 (46%) out of 76 seropositive with HBc Ab, the age group (55-65) year showed high significant (p<0.05) than other age groups, and male more infection than female. The result also revealed that the autoimmune hepatitis disease was 5 (6.5%) out of 76 patient infected with Type 1autoimmune hepatitis. Complement fractions C3, decreased in all patients compared to those of a healthy control, while the autoimmune hepatitis patient recorded high significant increase at (P<0.05) was (142.2±8 mg/dI) .In regard to C4 was revealed normal concentration in all patients compared with control groups while the autoimmune patient recorded high significant (P<0.05) was (41.7±5.1 mg/dI) and non-significant (P<0.0) in males than female in concentration of (C3 and C4).

2015

- Assessment of Viral load for Human Cytomegalovirus in Patients with Hepatitis B virus

الباحثونHuda j.b.AL Khilkhali، Mohammed A.Muhsin Mohammed A.K.ALSaadi
المجلةAL- Kufa University Journal of Biology/VOL.7/NO.3/Year:2015.
مختصر البحث

This study was carried out on Seventy-Six samples, formed (55 males and 21 females) with age ranging (11-72) year were collected from Center Health Laboratory/Al-Hakeem Hospital, and AL-Sadder medical city in AL-Najaf city, during the period from January (2013) to August (2013). Fifteen healthy individuals without any evidence of chronic inflammatory disease involved as control, age ranging (21-50) years. All patients and divided in six age groups. Blood samples were collected from patients and control for immunological (IgM, IgG & TFN-α) by using ELISA and molecular study by RT-PCR, respectively. The results showed that 76 HBsAg seropositive in all age groups but the age group (44-54) year revealed high significant(p<0.05) than other age groups and male more infection than female. The ELISA test results showed that 68 (89.4%) out of the 76 samples were positive for anti-HCMV IgG antibodies. And 4(5.26%) samples were positive for anti-HCMV IgM. The results of the Real-Time PCR revealed that HCMV DNA were detected in 23(30.2%) out of 76 patients were found in all age groups with viral loads ranging from (0.24- 1730000) Copies/ml, and the results of controls group in Real-Time PCR were HCMV negative. The results of cytokines profile (TNF-α) showed a highly significant (P<0.05) elevation in the serum of all patients than control groups.

المحاضرات

10

الأخبار والإعلانات

2
الأخبار 2022-12-04

المشاركة في المؤتمر الدولي الاول للعلوم الصرفة في جامعة بابل

المشاركة ببحث Antimicrobial susceptibility patterns of Klebsiella pneumoniae isolated from older smokers and non-smokers of inpatients in intensive care unit infected with chronic pneumonia in AL-Najaf hospital, Iraq

الأخبار 2017-10-29

المشاركة في ندوة علمية بعنوان "الاحياء المجهرية وتاثيرها على الانسان والبيئة"

ستقام في الكورس الثاني في قسم التحليلات المرضبة

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