Evaluation of Oxidative Stress in Molecular Diagnostic Patients with Toxoplasma gondii in A Najaf province
AuthorsFatima Fairs Mailk, Muntazer Muhammad Ibrahim, Zahraa Sajed Hussien, Rasha Amer Nouri Al-Tufaili
JournalEurasian Medical Research Periodical
Abstracttoxoplasmosis is caused by infection with Toxoplasma gondii. This coccidian protozoan is normally transmitted to humans by ingestion of either oocysts from cat feces, or by tissue cysts in raw or undercooked meat It is one of the most important problems in immunocompromised individuals. The frequency of primary maternal toxoplasma infection depends on the proportion of seronegative pregnant women who are susceptible to infection and on the prevailing infection risk. In the study you mentioned, the researchers likely used molecular diagnostic techniques to evaluate the presence of T. gondii in patients in Najaf province. They may have also assessed oxidative stress in these patients, which is a type of cellular damage caused by the production of reactive oxygen species (ROS). Elevated levels of ROS can cause damage to DNA, proteins, and lipids, and may be associated with the development of various diseases. The researchers may have measured markers of oxidative stress, such as certain enzymes or biomolecules, in order to understand the potential relationship between T. gondii infection and oxidative stres
Evaluation of Malondialdehyde as a biomarker
in investigation of Toxoplasma gondii in
infected women
AuthorsDuaa A. Sulaiman, Rasha Amer Noori
JournalInternational Journal of Health Sciences
AbstractAbstract---This study was aimed to estimate the prevalence of
Toxoplasma gondii infection and associated risk factors among
patients in Al-Najaf Province and to investigate whether there is a
relationship between this infection and (MDA). A total of 117 blood
samples were collected from Iraqi women with toxoplasmosis at AlZahra Teaching Hospital for Maternity and Children in AL-Najaf
province. The PCR test was carried out in the Advanced Research
Laboratory of the Department of Laboratory Investigations / Faculty of
Science – University of Kufa. The estimation of MDA marker level in
patients and 30 healthy women (control group) was conducted in AlAmeen Center for Research and Advanced Biotechnology/ Imam Ali
Holy Shrine. The results of the PCR (to confirm the toxoplasmosis
infection) had shown that 53(45.30%) patients were found positive
and 64 (54.70%) were negative. The results of biochemical study
showed that the concentrations of MDA level were increased in
toxoplasmosis patients compared with the controls. The results of this
study highlight on the wide spread of Toxoplasmosis in Iraq, especially
in Najaf Governorate. The use of molecular methods to diagnose
toxoplasmosis remains the most accurate technique for diagnosing
infection.
Role of nitric oxide in detection toxoplasmosis
patients in Al-Najaf Province
AuthorsDuaa A. Sulaiman, Rasha Amer Noori
JournalInternational Journal of Health Sciences
AbstractAbstract---This study was aimed to estimate the prevalence of
Toxoplasma gondii infection and associated risk factors among
patients in Al-Najaf Province and to investigate whether there is a
relationship between this infection and one of oxidative stress marker
level (nitric oxide (NO). A total of 117 blood samples were collected
from Iraqi women with toxoplasmosis at Al-Zahra Teaching Hospital
for Maternity and Children in AL-Najaf province. The PCR test was
carried out in the Advanced Research Laboratory of the Department of
Laboratory Investigations / Faculty of Science – University of Kufa.
The estimation of NO marker level in patients and 30 healthy women
(control group) was conducted in Al-Ameen Center for Research and
Advanced Biotechnology/ Imam Ali Holy Shrine. The results of the
PCR (to confirm the toxoplasmosis infection) had shown that
53(45.30%) patients were found positive and 64 (54.70%) were
negative. The results of biochemical study revealed that the
concentrations of NO level were increased in toxoplasmosis patients
compared with the controls. The results of this study highlight on the
wide spread of Toxoplasmosis in Iraq, especially in Najaf Governorate.
The use of molecular methods to diagnose toxoplasmosis remains the
most accurate technique for diagnosing infection. The results of the
study documented that there is a relationship between high levels of
oxidative stress markers (NO) and infection with T. gondii.
CLINICAL DIAGNOSTIC COMPARISON OF ENTAMOEBA HISTOLYTICA
IN IRAQI PATIENTS
AuthorsRasha Amer Noori Al-Tufaili
JournalPlant Archives Vol. 20, Supplement 2, 2020 pp. 601-603 e
AbstractEntamoeba histolytica is the responsible amöbiasis parasite and remains one of the top three parasite mortality triggers worldwide. With increased travel and mass migration to developed countries, infection in nonendemic regions is becoming more widespread. Though most people were afflicted with E. histolytica stays asymptomatic, some with amöbian colitis and spread illness. As more is known about their etiology and the inflammatory response of the host, there is hope for the ability to create a vaccine. This study has been related with investigation of this parasite and several parameters like: age, sex and occupatio
Evaluation of oxidative stress and growth status in
molecular diagnostic patients with entamoeba histolytica
AuthorsRasha Amer Nouri Al-Tufaili , , Rasha Shakir Nima 2
JournalEurAsian Journal of BioSciences
AbstractIn the developing world, amoebiasis is a widespread parasitic disease in which Entameoba histolytica is the causative agent for a human. The aim of this study is to evaluate the end products of oxidative stress include; serum levels of malondialdehyde (MDA), and serum levels of total protein carbonyl (POC) in Entameoba histolytica patients. In addition to estimate the growth retardation of children infected with this parasite. In the current study the sera and stool of 37 patients with Entameoba histolytica infection and 37 healthy children in the control group were diagnosed by Molecular detection techniques. Growth status were calculated and oxidative stress were analyzed. The mean age of the patient was 5.16±4.811 years. There were significant lower differences between the mean of z-score for high and mean z-score for weight in patient than that of controls. T-test demonstrated markedly higher MDA levels, and POC levels were spotted in serum of patients as matched to the patients with control group. It is concluded that the Entameoba histolytica infection was associated with significant oxidative stress and growth retardation.
A Phylogenetic Study of Entamoeba Histolytica Isolated
from Patients in the Babylon Hospital of Iraq Based on 18S
Ribosomal RNA Gene
AuthorsSaba Fadhil Ali Malaa, Rasha Amer Noori Al Tufaili, Dhamiaa Maki Hamza, Raad Ajem
JournalIndian Journal of Public Health Research and Development
AbstractAbout ten percent of the world’s people are infected with Entamoeba. histolytica, which representing over fifty million people annually. Sequences of 18S ribosomal RNA gene have been obtained for five new isolates of Entamoeba histolytica. which is represented new insights into the evolution of phylogenetic analyses of this parasite in Iraq. In the current study, the phylogenetic relationships between five local isolations of Entamoeba histolytica and eight Entamoeba spp global isolates were studied by using the18S rRNA gene sequenc
