ا.د.رغد علي صاحب الموسوي

Bacterial Vaginosis (BV) has recently emerged as a global health issue especially in pregnant women because of its adverse outcomes. Various studies have shown the impact of BV on both mother and baby as well as overall reproductive health of women. Objective: The aim of the study is To detect the effect of Silver Nanoparticles synthesized from Penicillium chrysogenum on Klebsiella pneumoniae isolated from patients with bacterial vaginosis. Methodology: 104 specimens collected from bacterial vaginosis patients from (September 2024 to February 2025) for isolation of Gram negative bacteria, to synthesis Silver Nanoparticles 5 mL of the fungal extract was added to the 100 mL of diluted silver nitrate solution. Results: Among the isolated Gram-negative pathogens, Klebsiella pneumoniae was the most prevalent species 21(20.19%), highlighting its significant role in infections associated with bacterial vaginosis and its resistance to multiple antibiotics. The effectiveness of silver Nanoparticles extracted and manufactured from the fungus, P. chrysogenum against Klebsiella pneumoniae, the Ultraviolet and visible spectroscopy (UV-Vis) was to confirm AgNPs which the UV-vis spectrum revealed the formation of wavelength peak for AgNPs is at (450nm) and absorption peaks at (1.6 nm), and also characterization by Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR) Analysis and X-Ray Diffraction Analysis (XRD). Conclusions: Silver nanoparticles produced by P. chrysogenum has high efficiency in inhibition, decreasing number and destroying the Klebsiella pneumoniae by AgNPs produced by this fungus at different concentrations (25, 50, and 100) μg / ml. The largest inhibition zone of AgNPs was (50 mm) by a concentration of (100 μg /ml), whereas the lowest inhibition zone was (12mm) at the concentration (25 μg/ ml).

Patients with renal failure suffer from Infection with some gram negative strains, as the development of bacterial resistance to antibiotics is a major problem that limits the use of antibiotics to treat diseases resulting from bacteria. Objective: To detect the effect of ZnO Nanoparticles synthesized from Penicillium chrysogenum On Acinetobacter species isolated from patients with renal failure. Methodology: 102 specimens collected from renal failure patients from (September 2024 to February 2025) for isolates gram negative bacteria, to synthesis nanoparticles 5 mL of the fungal extract was added to the 100 mL of diluted zinc nitrate solution. Results: Among the isolated Gram-negative pathogens, A. baumannii was the most prevalent species 20(19.6%), highlighting its significant role in infections associated with kidney failure and its resistance to multiple antibiotics.The effectiveness of Zinc Oxide Nanoparticles extracted and manufactured from the fungus , P. chrysogenum against A. baumannii, the Ultraviolet and visible spectroscopy (UV-Vis) was to confirm ZnONPs which The UV-vis spectrum revealed the formation of wavelength peak for ZnONPs is at (379 nm) and absorption peaks at (0.80 nm), and also characterization by Atomic Force Microscopy (AFM) , Fourier Transform Infrared Spectroscopy (FT-IR) Analysis and X-Ray Diffraction Analysis (XRD). Conclusion: Zinc Oxide produced by p. chrysogenum has high efficiency in inhibition, decreasing number and destroying the Acinetobacter species by ZnONPs Produced by this fungus at different concentrations (25, 50, and 100) μg /ml . The largest inhibition zone of ZnONPs was (45mm) by a concentration of (100 μg /ml), whereas the lowest inhibition zone was (11mm) at the concentration (25 μg/ ml).

Kidney failure patients suffer from the risk of developing gram negative infections, as the development of bacterial resistance to antibiotics is a major problem that limits the use of antibiotics to treat diseases resulting from bacteria.from this perspective, our research aims to isolate and diagnose Acinetobacter baumannii and ability to produce genes antibiotics resistance. Methodology: 102 specimens were collected from kidney failure patients in AL- Najaf Governorate. Iraq, from (September to November 2024). The types of gram-negative bacteria identified using biochemical testing and vitek 2 system . Specimens. All specimens were grown on MacConkey agar , using Selective media for Acinetobacter, CHROMagar Acinetobacter and Leeds Acinetobacter Medium. extracted using the Wizard® Genomic DNA Purification Kit, PCR testing was performed to detect genes. Results: Out of a total of 102 patients diagnosed with kidney failure, Gram-negative bacteria were isolated from 92 specimens (90.19%), while the remaining 10 (9.80%) showed no bacterial growth. kidney failure were most prevalent in the 50-59 age group, with 40 patients (39.21%) affected, and the least affected group was 10-19 years old. Molecular analysis revealed the presence of key virulence genes, including SurA1, OmpA and bas D . The SurA1 gene was detected in all isolates, suggesting its critical role in bacterial survival and pathogenicity. In contrast, the OmpA gene appeared in fewer isolates, indicating genetic diversity among strains.Conclusion: These findings emphasize the potential of SurA1 as a reliable virulence marker in clinical isolates of A. baumannii and bas D gene found five isolates out of ten isolates.

Bacterial vaginosis (BV) is a disease caused by an imbalance of vaginal microecology , Vaginal flora is one of the most important defense mechanisms for reproductive function and maintaining ahealthy environment Healthy vaginal flora is composed of anaerobic bacteria and aerobic bacteria, with Lactobacillus as the dominant microorganism, . Out of a total of 104 patients diagnosed with bacterial vaginosis , Gram-negative bacteria were isolated from 86 specimens (82.69%), while the remaining 18 (17.30%) showed no bacterial growth. Bacterial vaginosis were most prevalent in 3rd trimester of gestational period in compared with other groups of gestational period, 70 (67.3%), and lowest at 1st trimester 10(9.62) . The results of the pure gram-negative isolates indicated that K. pneumonia was the most dominate bacteria with 21(20.19%) isolates followed E. coli by 19(18.26%) isolates, and lowest P.aeruginosa 3(2.88%) . Molecular analysis revealed the presence of key virulence genes, including magA ,rmpA.iucC ,ana K2F the four genes were found in 90% 0f these isolates .

The samples of C. albicans were selected for a sensitivity test with three concentrations were selected, as follows (50 ,100 ,200) mg/L. the upper inhibition zone is 200 mg/L which increased as compared with other concentrations, the inhibition percentage used to compare between all data using SPSS V.25 computer software using a T-test. P - values of less than 0.05 was considered statistically significant. Green synthesis using to produce silver nanoparticles by Syzygium aromaticum after adding the plant extract obtained of a silver nanoparticles when adding 4ml extract of S. aromaticum and the best pH to produce (AgNps) is 8 with wavelength 435 nm and 1 molarity by UV-visible devise. Field emission-scanning electron microscope (FESEM) revealed damage of the cells and inhibition biofilm formation of C.albicans when treated with AgNPs produce by S. aromaticum as compared with control of C.albicans which un treated with AgNPs,the inhibition growth diameter is 19mm when using 200 mg/L caused the upper inhibition zone as compared with other concentrations.

The current study, which is carried out on patients with renal failure in Al-Najaf province, 200 oral samples were collected from the dialysis halls in males and females. In both genders, 75 positive samples were found,females have been shown to have a higher rate of Candida infection than males. Females have 48 positive samples out of 100, while males have 27 positive samples out of 100, The percentage of females in positive samples is 64 %, whereas the rate of males is 36%. A positive diagnosis of samples is made using molecular identification of C.albicans by PCR The results of HPLC show ability of C.albicans to produce secondary metabolites represented by a similar structure of gliotoxin (GT), 16.33 µg/ml concentration is obtained as the highest concentration in the sample15 grown on Minimum Essential Medium (MEM) and low concentration in a sample 1 cultured on Sabouraud Dextrose Brot (SDB) is 1.568 µg/ml. The secondary metabolite of the extract was examined by FTIR, and it was found that there is a significant homogeneity in the active groups in GT.

نظّم قسم علوم الحياة في كلية العلوم حلقة نقاشية بعنوان “البوتوكس والفيلر في التجميل والعلاج” قدّمتها الأستاذ الدكتورة رغد علي صاحب، وتناولت فيها ماهية هاتين المادتين واستخداماتهما الطبية والتجميلية. شرحت المحاضِرة أن البوتوكس مادة مشتقة من سم بكتيريا Clostridium botulinum، تعمل على إرخاء العضلات مؤقتًا من خلال منع انتقال الإشارات

بحثت رسالة ماجستير في قسم علوم الحياة تصنيع دقائق الفضة النانوي المصنع بيولوجيا باستخدام الفطريات ضد بكتريا Klebsiella pneumoniae المعزولة من النساء الحوامل المصابات بالالتهابات البكتيرية Vaginosis ودراسة الجينات المسببة لعوامل الضراوة والمقاومة المضادات K2A, rmbA, magA . iucC, وتهدف الرسالة التي قدمتها الباحثة نور كريم مدلول باشراف الأستاذ الدكتور رغد علي صاحب

بحثت رسالة ماجستير في قسم علوم الحياة تصنيع دقائق أوكسيد الزنك النانوي المصنع بيولوجيا بأستخدام فطر penicillium chrysogenum ضد بكتريا Acinetobacter baumannii المعزولة من مرضى الفشل الكلوي وتهدف الرسالة التي قدمتها الباحثة نوران جعفر حسن باشراف الأستاذ الدكتور رغد علي صاحب الى عزل و تشخيص بكتريا السالبة الغرام المنتشرة بين مرضى الفشل الكلوي

بحثت اطروحة دكتوراه في قسم علوم الحياة تصنيع دقائق الفضة النانوية بيولوجيا باستخدام فطر Rhizopus stolonifer، ضد البكتيريا شديدة المقاومة للمضادات الحيوية. وتهدف الاطروحة التي قدمها الباحث منتظر رياض لفته باشراف الاستاذ الدكتور رغد علي صاحب، الى ايجاد طريقة امنة للعلاج بتصنيع دقائق الفضة النانوية من الفطريات ضد البكتيريا المقاومة وبتراكيز منخفضة واقل سمية وتجريبها على خط الخلايا الليفية الجلدية.

نظم قسم علوم الحياة حلقة نقاشية عن استخدام تقنيات الذكاء الاصطناعي في مجال التشخيص وتحليل البيانات في علم الاحياء المجهرية. وشهدت الحلقة القاء محاضرة للاستاذ الدكتورة رغد علي صاحب تناولت فيها تطبيقات الذكاء الاصطناعي في مجال الاحياء المجهري وخاصة في تحليل عينات البيانات المختلفة مثل الدم والادرار والانسجة لتحديد العدوى المجهرية ومقاومة المضادات الحيوية، وكذلك استخدام الذكاء الاصطناعي في تطوير العلاجات وخاصة العقاقير التي تستهدف الكائنات المجهرية

بحثت رسالة ماجستير في قسم علوم الحياة اهم الجينات المسؤولة عن تكوين البايوفلم و مقاومة المضادات الحيوية في بكتيريا المكورات العنقودية الذهبية، وتأثير دقائق الفضة ِالنانوية المصنعة من الفطر المحاري في تثبيط نمو تلك البكتيريا المعزولة من مرضى القدم السكري. وهدفت الرسالة التي قدمها الطالب ضرغام عبد الرضا عباس بإشراف ا.م.د رغد علي صاحب، الى الكشف عن جينات الضراوة(ICAD,HLA,MECA,IECA) التي تساعد بكتريا المكورات العنقودية الذهبية على البقاء في جسم المريض،