رافع شاكر عبود الخفاجي

Abstract Objective: In vitro fertilization (IVF) is one of the several assisted reproductive technologies (ART) used to aid infertile couples to conceive a child. Many factors affecting the rate of success of IVF cycle. The aim of the present study was to assess the correlation of the presence of anti-zona pellucida antibodies (AZPA) and the outcome of IVF. AZPA also was correlated with hormone levels in women with IVF failure. Methods: Sixty women undergo IVF were participated in the study. Women group that had conceived from IVF are expressed as "pregnant group" while women who hadn’t are expressed as "failure group". All groups had treatment regimen depending on parameters. Women with IVF failure were devided into subgroups according to the total gonadotrophin dosage, duration of treatment, and oocyte yield. Results: Results revealed that there is the insignificant difference between pregnant and failure groups in serum AZPA level. The results showed a significant higher estradiol (E2) (p=0.024), anti-müllerian (AMH) (p=0.006), and lower prolactin (PRL) (p=0.002) in pregnant in comparing with failure group. Correlations studies indicated a significant correlation between AZPA and AMH in pregnant group, and there is a significant correlation between AZPA and E2 hormone in the failure group. Conclusion: It can be concluded that serum AZPA level does not differ between women who had success or failure IVF. However, in women with failure pregnancy in IVF, AZPA is positively correlated with E2 and its negatively correlated with PRL. In pregnant group, AZPA is positively correlated with AMH.

Autosomal dominant polycystic kidney disease (ADPKD) is the most common life threatening hereditary disease of the kidney. It is a systemic disease characterized by multiple, bilateral renal cysts that result in massive renal enlargement and progressive functional impairment. Patients with ADPKD often develop hypertension before any abnormalities in renal function are detected clinically. In particular, hypertension is insidious and remains a continuous problem that evolves during the course of the disease. Atrial natriuretic peptide (ANP) possess bioactive on blood pressure. This study dealt with two groups of patients with ADPKD; the first included the patients with renal failure while the other included the patients without renal failure, as well as healthy control group. The results found prevalence of hypertension in patients with ADPKD, reached 75%. Blood pressure was significant increase (P≤0.05) in ADPKD patients without renal failure than control, where reached to 137.14/85 mmHg compared with 123.93/80.71 mmHg respectively. The ANP levels were significant increase (P≤0.05) in ADPKD patients with renal failure compared with ADPKD patients without renal failure and control groups, which reached (106.5 pg/ml), (79.2 pg/ml) and (24.4 pg/ml) respectively. Key words: Autosomal Dominant Polycystic Kidney Disease, hypertension, Atrial Natriuretic Peptide.

تѧѧѧѧم فѧѧѧѧي هѧѧѧѧذه الدراسѧѧѧѧة اختبѧѧѧѧار فعاليѧѧѧѧة المѧѧѧѧستخلص الايثѧѧѧѧانولي لأوراق نبѧѧѧѧات الطرطيѧѧѧѧع aegyptiaca Schanginia فѧѧي بعѧѧض جوانѧѧب حياتيѧѧة بعوضѧѧة quinquefasciatus Culex واعتبѧѧرت نѧѧسب الهѧѧلاك ومѧѧدة نمѧѧو الأدوار غيѧѧر البالغѧѧة وإنتاجيѧѧة الإنѧѧاث مѧѧن البѧѧيض ونѧѧسبة فقѧѧسه معѧاييرا" للاختبѧار.حيѧث بلغѧت هلاآѧات البيѧوض 9.21 %فѧي الترآيѧز 2 ملغѧم/مѧل مѧن المѧستخلص 124 ,وآان الطوران اليرقيان الأول والثاني أآثر تأثرا بتراآيز المѧستخلص حيѧث بلغѧت هلاآѧا تهمѧا 90 % و6.80 %على التوالي,بينما بلغت هلاآѧات الطѧورين اليѧرقيين الثالѧث والرابѧع 52 %و6.39 %علѧى التѧوالي فѧي الترآيѧز أعѧلاه,وبلغѧت هلاآѧات العѧذارى 29 %فѧي ذات الترآيѧز. بلѧغ الهѧلاك التراآمѧي للأدوار غير البالغة 100 %في الترآيز 1 ملغم/مل.وأدى التأثير التراآمي للمستخلص إلى إطالѧة مѧدة نمو الأدوار غبر البالغة بحيث بلغت 4.19 يوما"بالمقارنة مع 4.12 يوما" لمعاملѧة الѧسيطرة.وخفѧض إنتاجية الإناث مѧن البѧيض حيѧث آانѧت بمعѧدل 154 بيѧضه/قѧارب بالمقارنѧة مѧع 7.200 بيѧضه/قѧارب معدل إنتاجية معاملة السيطرة, آذلك انخفاض معدل نسب فقس البيض إلى 2.89 %مقارنة"مѧع نѧسبة فقس بيض معاملة السيطرة التي بلغت 9.97 .%

لخلاصة In this study activity tested of ethanolic extract of Schanginia aegyptiaca leaves on some biological aspects of Culex quinquefasciatus mosquito, mortality rates, development period, productivity and hatchability 131 were chosen as criteria for biological testing. The results showed eggs mortality rate reached 21.9% at 2 mgml concentration ,the first and second larval instars more affecting than third and fourth larval instars ,were reached 90% and 80.6% comparative with 52% and 39.6% respectively at the same concentration, while pupal mortalities reached 29% at same concentration. Accumulative mortalities of immature stages reached 100% at 1mgml concentration. Accumulative effecting due to extended development period was reached 19.4 days comparative with 12.4 days 0f control treatment ,and reduced productivity of eggs to 154 eggs aft and decreased hatchability too, was reached 89.2% comparative with 200.7eggs aft productivity and 97.9% hatchability for control treatment.

لخلاصة In this study activity tested of ethanolic extract of Schanginia aegyptiaca leaves on some biological aspects of Culex quinquefasciatus mosquito, mortality rates, development period, productivity and hatchability 131 were chosen as criteria for biological testing. The results showed eggs mortality rate reached 21.9% at 2 mgml concentration ,the first and second larval instars more affecting than third and fourth larval instars ,were reached 90% and 80.6% comparative with 52% and 39.6% respectively at the same concentration, while pupal mortalities reached 29% at same concentration. Accumulative mortalities of immature stages reached 100% at 1mgml concentration. Accumulative effecting due to extended development period was reached 19.4 days comparative with 12.4 days 0f control treatment ,and reduced productivity of eggs to 154 eggs aft and decreased hatchability too, was reached 89.2% comparative with 200.7eggs aft productivity and 97.9% hatchability for control treatment.

هدفت هذه الدراسة الى إمكانية إيجاد وسيلة فعالة لحماية مخازن الحبوب من الإصابة بخنفساء الحبوب الشعرية ( الخابرا) Trogoderma granarium من خلال اختبار كفاءة بعض العوامل الحيوية المتمثلة بـ ( لقاح الفطر Beauveria bassiana و لقاح البكتريا thuringiensis Bacillus ) والكيمياوية المتمثلة بـ ( مبيد ميتكول ، مبيد جنتابرايد و مبيد ميثان ). اظهرت نتائج الدراسة ان لقاح الفطر B.bassiana قد تفوق على لقاح البكتريا B. thuringiensis من حيث التأثير في نسب الهلاك اذ سبب نسبة هلاك بلغت 34.08 % مقارنة بـ27.98 % لمعاملة لقاح البكتريا B. thuringiensis بعد مرور 5 أيام من المعاملة. كما اظهر المبيد الكيمياوي ميتكول تفوق على مبيدي جنتابرايد وميثان من حيث التأثير في نسب الهلاك اذ سبب نسبة هلاك بلغت 34.34 % مقارنة بـ ( 25.51 و 32.63 ) % لمبيدي جنتابرايد وميثان على التوالي ، بعد مرور 5 أيام من المعاملة . أوضحت نتائج التجارب المختبرية إن التركيز 10 مل / لتر ماء، للمعاملات الحيوية قد سبب أعلى نسبة هلاك حيث بلغت %47.89, مقارنة بـ 1.72 % في معاملة السيطرة, و بتفوق معنوي على جميع التراكيز الأخرى, كما أظهرت التراكيز الأخرى فعالية جيدة في مكافحة الحشرة,. كما أظهرت الأطوار اليرقية المختلفة حساسية متباينة للمعاملات الحيوية والكيمياوية ، وكان الطور اليرقي الخامس اقلها تأثرا متمثل ذلك بإعطائه اقل نسبة هلاك والبالغة 22 % للمعاملات الحيوية و %4.26 للمعاملات الكيمياوية .

This study was evaluate conducted to the hexane extract effect of Schanginia aegyptiaca leaves on some biological aspects of Culex quinquefasciatus mosquito. The mortalities, development period ,productivity and hatchability were chosen as criteria for bioassay. The results revealed that egg mortality was 18.7% at 2 mg/ml,while the 1st,2nd,3rd and 4th larval instars were 75.9% ,62.3% , 48.3% and 46.7% respectively at the same concentration.Pupal mortality was 25.5% at the same concentration too. Accumulative mortality of immature stages was 100% at 1mgml while the developmental period was extended to 17.5 days in comparison with 12.4 days of control. Treated female productivity was169.2 eggsaft comparative with 200.7 eggsaft of control Hatchability reduced to 91.6% comparative with 97.7% of control. أجريت الدراسة الحالية لتقييم تأثير مستخلص الهكسان لأوراق نبات الطرطيع Schanginia aegyptiaca في بعض جوانب حياتية Culex quinquefasciatus . واعتبرت نسب الهلاك لادوار الحشرة غير البالغة والأثر التراكمي ومدة النمو و الإنتاجية ونسبة الفقس كمعايير للتقييم,حيث أظهرت النتائج أن المستخلص اثر بشكل معنوي في هلاك الأدوار غير البالغة ,حيث بلغت نسبة هلاك البيوض 18.7% ونسب هلاك الأطوار اليرقية الأول,الثاني,الثالث والرابع 75.9و 62.3 و 48.3 و46.7% على التوالي في التركيز 2 ملغم/مل ,بينما بلغت نسبة هلاك العذارى 25.5% في ذات التركيز. وأدى التأثير التراكمي إلى نسبة هلاك بلغت 100% في التركيز 1 ملغم/مل , وكذلك إطالة في مدة نمو الأدوار غير البالغة حيث بلغت 17.5 يوما" بالمقارنة مع معاملة السيطرة التي بلغت 12.4 يوما" , في حين أدى المستخلص إلى خفض إنتاجية البالغات من البيض وكذلك نسبة فقسه حيث كانت 169.2 بيضه/قارب و91.6 % على التوالي مقارنة" مع معاملة السيطرة التي بلغت إنتاجيتها 200.7 بيضه/قارب وبنسبة فقس 97.9%.

Autosomal dominant polycystic kidney disease (ADPKD) is the most common Mendelian disorder of the kidney and affects all racial groups worldwide. It is characterized by focal development of renal and extrarenal cysts in an age-dependent manner. This study tested some physiological parameters in two groups of patients with ADPKD, the first group included patients with kidney failure and the second group included patients without renal failure as well as the control group. The study showed an increase in urea and uric acid in the serum of the patients without renal failure compared with the control levels and were higher in the patients with renal failure compared with the patients without renal failure, which amounted to 115.8 mg / dL and 10,278 mg / dL and 22.45 mg / dL and 7,264 mg / dL and 11.03 mg / dL and 3,264 mg /dL respectively. Creatinine serum level was higher in the patients with renal failure compared with the patients without renal failure and control, reaching 3.5 mg / dL , compared with 1,026 mg / dL and 0986 mg / dL , respectively. Potassium ion level was higher in patients without renal failure than in control reaching 4,179 mmol / L and 2.34 mmol / L, respectively, while the level was higher in patients with renal failure than in patients without renal failure where it reached 7.09 mmol / l. Sodium ion levels were low in the patients with renal failure and the patients without renal failure than in the control group, reaching 87.06 mmol / l and 129 843 mmol / l and 147.25 mmol / L, respectively. The level of sodium ion was lower in the patients with renal failure than in the patients without renal failure. Results of the study showed normal levels in serum albumin and liver enzymes, AST and ALT