نور الهدى ناظم صادق الغزالي

Colorectal cancer (CRC) is the third most prevalent form of cancer and the second leading cause of cancer-related deaths worldwide. oncolytic virotherapy shows promise as a targeted therapy for various cancer types, including CRC. In this study, we constructed a novel oncolytic herpes simplex virus type 2 (oHSV-2) are derived from the wild type HSV-2, that isolated from semen samples of suspected infertile men. We investigated the effects of oHSV2 in vitro on expression the Carcinoembryonic antigen (CEA) in LS174T colorectal adenocarcinoma cell line model compare with a normal vero cell line. Tumor markers expression monitoring by the enzyme-linked immunosorbent assay (ELISA), of untreated cells and that treated with wHSV-2, oHSV-2 and after treatment with the traditional chemotherapeutic agent 5 flouro uracil 5FU. The results demonstrated a downregulation and decline in the concentration of Carcinoembryonic antigen (CEA), these results were statistically non-significant (p= 0.302) when compared with control (untreated cells) level of CEA,(p= 0.302). The drug 5FU revealed CEA down regulation with positive correlation coefficient with dose dependency (r2= 0.9797) and IC50 of 47.29 µg/ml. we concluded that our virus is a safe and effective therapeutic agent for down regulation and decline in the concentration of Carcinoembryonic antigen (CEA).

This research was conducted on 48 women who suffered from spontaneous abortion for one and multiple fold during the first and second stage of pregnancy and with age averages ranging from 14-43 years. which have been confirmed to be infected with the Human Cytomegalovirus (HCMV) by using the Enzyme Link Fluorescent Assay technique (ELFA), However, they did not suffer from chronic diseases such as blood pressure, diabetes and other immune diseases, In order to measure the level of interleukin (IL-10) by the enzyme-linked immunosorbent assay (ELISA) in blood samples, placenta and amniotic fluid that collected from women at the time of abortion and while they were in the Curettage Hall of Al-Zahra Hospital and Al-Hakim Teaching Hospital/Najaf Governorate, For the period from October 2015 to April 2016, comparison was made with 10 women who had no history of previous miscarriage or inflammatory disease and had been as control group. The results showed no significant differences between the level of the inflammatory inhibition factor interleukin (IL-10), that measured in the serum of abortive women and the control group, which reaching the level to (5.905±1.5707) Pg/ml. also noted high Significant decrease P≤(0.01) in the interleukin (IL-10) concentration that measured in the placenta and amniotic fluid of abortive women compared to healthy Control group. From the above results, concluded that the decrease level of interleukin (IL-10) in abortive women infected with virus may be plays a major role in cases of spontaneous abortion.