نصير جواد كاظم كاظم

Abstract High selectivity of Cytotoxic Activity of Compounded Anthracycline against Rhabdomyosarcoma Cancer Cell Line that effect of DMT between cancer cell and normal cell line increase the prospects that this compound could serve as leads for novel anticancer drugs and hence may be potential candidates for use in human cancer therapy, however, further investigations will be required in this regard to validate this hypothesis.

Background/aim: Organophosphorus (OP) compounds contain a heterogeneous types of chemicals particularly designed for the command of pests, weeds or plant diseases. The present study mainly focused on the possible potential chemoprotective role of 20% methanolic extract of Curcuma longa L. rhizomes against biochemical toxicity following dichlorvos exposure for 35 days in albino rats (females). Materials and methods: Female albino rats were divided into four groups of fifth rats each: Group I served as control. Group II was administered orally with dichlorvos (DDVP) 10 mg/kg body weight. Group III received 20% methanolic extract of C. longa L. rhizomes 500 mg/kg body weight. Group IV received both DDVP 10 mg/kg body weight plus 20% methanolic extract of C. longa L. rizhomes 500 mg/kg body weight. All animals were scarified after 35 consecutive days of the beginning of the experiment. Samples of blood were taking for biochemical analysis which include total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), triglycerides (TG), total proteins (TP), and urea concentrations. Results: The data of present study demonstrated that plasma total cholesterol, triglycerides, and HDL-C concentrations were non-significantly increased whereas the LDL-C level significantly increased (p<0.05) by DDVP treatment. Also, non–significant differences was seen in VLDL-cholesterol concentration between groups. Total proteins concentration was significantly reduced (p< 0.05) compared with the control animals. Furthermore, renal markers such as urea concentration were significantly (p< 0.05) increase in DDVP treated rats. Co-administration of 20%methanolic extract of C. longa L. rizhomes was able to restore all the parameters were studied to near-normal values. Oral feeding of 20% methanolic extract of C. longa L. to DDVP-treated female rats at the dose of 500mg/kg body weight caused significant (p<0.05) decreasing of elevated parameters compared with DDVP group. Female rat treated with 20% methanolic extract of C. longa L. alone did not exhibited any significant differences. Conclusion: Treating rats with 20% methanolic extract of C. longa L. rizhomes caused improvement of DDVP negative effects on serum lipid profile, total protein and urea concentrations in comparison with control group.

Abstract DNA topoisomerases are ubiquitous, enzymes needed to control topological problems encountered during DNA replication, transcription, recombination and maintenance of genomic stability. They proved to be valuable targets for cancer chemotherapy. The seeds alkaloids extract of Rue plant (Peganum harmala L. Zygophyllaceae) were fractioned by high performance liquid chromatography as a suitable technique, HPLC waters company, gradient, Nova-Pak, C18*, 4µm, 3.9x150mm, mobile phase ACN-water pH10.7. Thirty-four separate solution depending on the retention time of eluents. The aim of this study is to investigate the trace compounds relatively and used as inhibitors for the topoisomerase II enzyme, the study the poison and suppressor model inhibitions and the synergy and antagonism activity of fractions. The visicinone, harmine, visicine and harmaline, were (2.86, 0.75, 13.14, and 16.62 mg/100mg of plant powder. The harmine ˃vicicinone˃ harmaline˃ vicicine as enzyme inhibitors. The unknown trace compounds gave the best inhibition relatively, the alkaloid fractions gave higher inhibitory units of fraction effective (FE). Results of seeds alkaloid fractions showed that the FE in state of fraction mixture was 36% inhibitory units, while summation of FE of each fraction alone gave higher level than their mixture, where sum of FE of them was 1229% inhibitory units. The suppressor inhibition model according to highest percent of the remain supercoiled DNA plasmid than production of linear and open nicked circular with high value in F8 (84%), the poisons inhibition model activity according to the highest percent of linear and nicked open circular DNA plasmid with high level in F31 (88%). There are a trace

15 ‫العدد‬ 3 ‫سنة‬ 2010 35 ‫كيم‬ ‫دراسة‬ ‫ي‬ ‫ا‬ ‫ئ‬ ‫ية‬ ‫لمسحوق‬ ‫الزنجبيل‬ ‫رايزومات‬ ‫الجافة‬ Zingiber officinale ‫و‬ ‫دورها‬ ‫أ‬ ‫في‬ ‫ونمو‬ ‫نبات‬ ‫الحنطة‬ Triticum aestivum ‫ثامر‬ ‫جميل‬ ‫اطياف‬ ‫سعديةياسرعوفي‬ ‫نصيرجوادكاظم‬ ‫العلوم‬ ‫كلية‬ ‫البيطري‬ ‫الطب‬ ‫كلية‬ ‫العلوم‬ ‫كلية‬ ‫الكوفة‬ ‫جامعة‬ ‫القادسية‬ ‫جامعة‬ ‫الكوفة‬ ‫جامعة‬ :‫الخالصة‬ ‫اجريت‬ ‫تجربة‬ ‫الزجاجي‬ ‫البيت‬ ‫في‬ ‫من‬ ‫للفترة‬ 10 / 11 / 2009 ‫لغاية‬ 10 / 12 / 2009 ‫عن‬ ‫للتحري‬ ‫تاثيرا‬ ‫الزنجبيل‬ ‫رايزومات‬ ‫مسحوق‬ ‫ضافة‬ Zingiber officinale ‫الجافة‬ (‫بالتراكيز‬ 12,8,4, /‫غم‬ ‫الحنطة‬ ‫ونمو‬ ‫أنبات‬ ‫في‬)‫تربة‬ ‫كغم‬ ‫لكل‬ Triticum aestivum ‫(بدون‬ ‫المقارنة‬ ‫معاملة‬ ‫عن‬ ‫فضال‬) ‫اضافة‬ ‫تضمنت‬ ‫كما‬ ‫التجربة‬ ‫للمركبات‬ ‫فصل‬ ‫الكيميائية‬ ‫الرقيقة‬ ‫الطبقة‬ ‫كروماتوغرافيا‬ ‫طريقة‬ ‫بأستعمال‬ Thin Layer Chromatography (TLC). ‫كشفت‬ ‫ال‬ ‫نتائج‬ ‫تجربة‬ ‫معنوية‬ ‫زيادة‬ ‫وجود‬)‫(سم‬ ‫الخضري‬ ‫المجموع‬ ‫استطالة‬ ‫في‬ ‫الجاف‬ ‫ووزنه‬)‫(ملغم‬ ‫الورقية‬ ‫والمساحة‬ ‫االوراق‬ ‫وعدد‬ ‫(سم‬ 2) ‫والوزن‬ ‫ين‬ ‫التركيزين‬ ‫في‬)‫(ملغم‬ ‫الخضري‬ ‫للمجموع‬ ‫الطري‬ ‫الوزن‬ ‫في‬ ‫معنوية‬ ‫زيادة‬ ‫وظهور‬)‫(ملغم‬ ‫الجذري‬ ‫للمجموع‬ ‫والطري‬ ‫الجاف‬ 8 ‫و‬ 12 ‫غم/كغم‬ ‫تربة‬ ‫عن‬ ‫فضال‬)‫ورقي‬ ‫نسيج‬ ‫(ملغم/غم‬ ‫الكلوروفيل‬ ‫محتوى‬ ‫أنخفاض‬ ‫التركيز‬ ‫في‬ 12 .‫تربة‬ ‫غم/كغم‬ ‫بين‬ ‫ال‬ ‫ت‬ ‫تجربة‬ ‫الكيمياوية‬ ‫عن‬ ‫على‬ ‫الريزومات‬ ‫أحتواء‬ (2) ‫من‬ ‫مركبات‬ Phenolics ‫و‬ (9) ‫من‬ Diterpenes ‫و‬ (6) ‫من‬ Sesequiterpen ‫و‬ ((11 ‫من‬ ‫مركبا‬ Tritepren ‫من‬ ‫واحد‬ ‫ومركب‬ Tetralkaloid ‫و‬ (4) ‫مركبات‬ ‫من‬ Alkaloid. :‫المقدمة‬ ‫أهمية‬ ‫تعود‬ ‫نباتات‬ ‫الزنجبيليات‬ ‫لعائلة‬ ‫العائدة‬ ‫االنواع‬ Zingiberaceae [‫االغريق‬ ‫زمن‬ ‫الى‬ 15 ‫والنتاج‬ ‫طبية‬ ‫أغراض‬ ‫لعدة‬ ‫واستعملت‬ ] [‫الصبغات‬ 2 1 [‫وكتوابل‬ ] 3. ] ‫النوع‬ Zingiber officinale ‫لالكسدة‬ ‫كمضاد‬ ‫الطبية‬ ‫منه‬ ‫متعددة‬ ‫استعماالت‬ ‫له‬ ‫للجلد‬ ‫ومقوي‬ skin tonic ‫االثيل‬ ‫وخالت‬ ‫الميثانول‬ ‫ومستخلص‬ ‫للرايزومات‬ ‫منه‬ ‫مضاد‬ ‫ة‬ ‫للسكري‬ antidiabetic [ ‫االنسولين‬ ‫هرمون‬ ‫الفراز‬ ‫ومحفز‬ 20 [ ‫اهمية‬ ‫االكثر‬ ‫الجزء‬ ‫هي‬ ‫النوع‬ ‫لهذا‬ ‫الرايزومات‬ ‫وتعد‬ ] 4 ] ‫يسمى‬ ‫طيار‬ ‫زيت‬ ‫لوجود‬ ‫باالساس‬ ‫تعود‬ ‫والفعالية‬ gingerol [ 3 ‫مثل‬ ‫اساسية‬ ‫وزيوت‬ ] monoterpen [ 23 .] ‫ك‬ ‫ان‬ ‫ما‬ ‫ل‬ ‫المائي‬ ‫لمستخلص‬ ‫للفطريات‬ ‫مضاد‬ ‫تاثير‬ ‫للرايزومات‬ antifungal [ ‫النباتية‬ ‫االنواع‬ ‫لبعض‬ ‫الممرضة‬ 24 [‫]و‬ 26 ]. ‫نتائج‬ ‫وبينت‬ [ ‫اجراها‬ ‫دراسة‬ 25 ‫مادة‬ ‫ان‬ ] glycine betaine ‫لفطر‬ ‫مضاد‬ ‫تاثير‬ ‫ذات‬ ‫الرايزومات‬ ‫من‬ ‫المستخلصة‬ Blumeria graminis ‫لمرض‬ ‫المسبب‬ powdery mildew ‫ا‬ ‫في‬ ‫للرايزومات‬ ‫وااليثانولي‬ ‫المائي‬ ‫للمستخلصين‬ ‫و‬ ‫لحنطة‬ ‫للنوع‬ ‫التابعة‬ ‫السلكية‬ ‫للديدان‬ ‫مبيد‬ ‫تاثير‬ Meloidgyne javanica [ ‫النباتات‬ ‫بعض‬ ‫جذور‬ ‫على‬ ‫المتطفلة‬ 9 ] ‫وتؤكد‬ ‫نتائج‬ [ 14 ] ‫تاثير‬ ‫يمتلك‬ ‫الذي‬ ‫النباتي‬ ‫المستخلص‬ ‫ان‬ a​n​t​i​f​u​n​g​a​l​