نجلاء عبد الامير علي الدهان

Typhoid fever is one of the most prevalent diseases in developing countries with high significant morbidity and mortality rates. In this study 120 patients with typhoid fever (males and females) aged 20 to 50 years, and 60 healthy individuals in the same age as a control group, from Al-Sader teaching medical city in Najaf/Iraq, from 1stMarch 2019 to the end of September2019 to assess their liver function and pro-inflammatory cytokines (IL-6 andIL-8)levels in their serum. Mean value to serum levels of liver enzymes: alkaline phosphatase (ALP), alanine transaminases (ALT) and aspartate transaminases (AST) were (130±9.8IU/l; 31.4±5.9IU/l; 26.8±4.2IU/l), respectively in Typhoid fever patients (P<.05 for each). Patients also showed significant increase in the concentration of IL–6 (157±5.2pg/ml), IL–8 (136±7.1pg/ml), and Creactive protein (CRP) 41±3.4 mg/l (P<0.001 for each) compared to healthy subjects 40±6.3 pg/ml; 37±5.9 pg/ml and 9±2.2 mg/l, respectively (P<.001).

Disorders of gall bladder are common and remain as one of the main patients’ chief complaints that might lead to surgical interventions. High level of bilirubin causes gallstones in patients with hereditary spherocytosis (HS). It is often misdiagnosed, because these deposits of bilirubin can be easily broken down in the gallbladder. Gallstones develop in less than half of patients with mild HS and do not always cause symptomatic biliary tract disease. HS is a genetic defect in red blood cells, caused by defects in structural membrane proteins. It is characterized by a decrease the surface area to volume ratio in erythrocytes due to the heterogeneous changes in the various genes that encode for proteins. A 14-year old male was brought to Medical Park hospital/Turki at the emergency room with a sudden onset of pain in the right upper quadrant, vomiting, anemia and jaundice. The patient was diagnosed with gallstone due to obstruction in common bile duct (CBD),with elevated bilirubin as a result for mild HS. This case report study highlight on the role of hereditary spherocytosis in forming many abdominal problem such as gallstone due to high level of heamolysis and elevated of bilirubin.

Background: Prostate cancer (PCa) is the most common cancer among men in western countries.Oxidative stress (OS) is considered to be involved in the pathophysiology of prostate cancer and development. Objective: To evaluate the OS in patients with PCa and its association with disease development. Materials and Methods: The current study included 130 patients with prostate cancer and 65 healthy subject (control). Lipid profile was analyses by commercially kit. Malondialdehyde (MDA) was determined by the thiobarbituric acid method. prostate-specific antigen (PSA), Superoxide dismutase(SOD), glutathione peroxidase (GSH-Px) activity and 8-Hydroxydeoxyguanosine(8-OHDG) levels were determined by enzyme linked immunosorbent assay(ELISA). Plasma vitamin E levels were estimated by colorimetric method according to the Meshali and Nightingale method. Results: Plasma TC,TG,LDL levels were significantly increased (P≤.001), while HDL levels was significantly lower in PCa patients compared with control(P=.338). There were a significant increases in PSA, MDA, 8- OHdG levels and SOD activity in PCa patients compared with control (P≤.001).Serum GSH-Px activity and plasma vitamin E levels were significantly decreased in PCa patients compared to control (P≤.001). PSA levels were positively correlated with MDA and 8-OHdG levels in PCa patients (r=.1700, p=.0332; r=.1059, p=.0271, respectively). A negative correlation was found between PSA and LDL in PCa patients (r=-.2657, P =.0310). MDA levels were negatively correlated with erothrocytes vitamin E levels(r=−.3780,p=.0142) and GSH-Px activity (r=−.4355, p=.0048), but positively correlated with erythrocyte SOD activity (r=.5376, p=.0003) and 8- OHdG levels (r=.6366,p=.0003). Conclusions: The results suggest an imbalance in oxidative stress and antioxidants system may lead to develop of PCa pathogenesis.

Introduction: Dental caries is one of the most common infectious diseases prevalent in the oral cavity, and it is an inflammatory disease with a multi-factor etiology. IL-6, IL-8, and TNF-α are two types of cytokines that are major mediators of acute inflammation, and are necessary for the development of specific immune responses. Objective: The present study aimed to assess the level of pro-inflammatory cytokines (IL-6,IL-8,and TNF-α) in the saliva of smokers with dental caries and the control (non-smokers) according to different age, BMI,and sex. Materials and Method: Whole saliva from 32 smokers aged 35-46 years with dental caries and 16 healthy subjects, to measure IL-6,IL-8, and TNF-a levels by enzyme immunoassay (ELISA). Results: The mean BMI, age, and sex between the experimental group and the control group were similar, were not statistically significant (p=0.466, p=0.240, p=0.455), respectively while, there were a significant difference in CRP between the two groups (p=0.037).Concentrations of salivary IL-6, IL-8 and TNF-α were not affected by periodontal health. No statistically significant difference in periodontal health, assessed by CPITN, were observed between the groups (p=0.413). Salivary levels of IL-6 and IL-8 were significantly higher in smokers with dental caries compared with control (p<0.05).Salivary levels of IL-6 and IL-8 were increased significantly in smokers with dental caries compared with control (p<0.05). No significant differences in salivary TNF-α in dental caries patients compared to control (p=0.063). The correlation between TNF-a, CRP, age, sex, and BMI with IL-8 were positive and statistically significant(p=0.01, p=0.036, p=0.009, p=0.007, and p=0.005), respectively. There was no significant correlation between IL-6 and TNF-a cytokines and other parameters, except for a positive association with CRP levels (p=0.006, p=0.043), respectively. Conclusions: These data indicate links between production of tumor necrosis factor-alpha (TNF-a), interleukin-6 (IL-6), and Interleukin-8 (IL-8)in smokers’ saliva and dental caries disease. Also,the existence of a statistically significant positive correlation between TNF-a,age, gender, and BMI with IL-8.

This study carried out in AL-Najaf province within Al-Manathera district that contents stream network,in dry season (2018),twenty samples collected and six physicochemical parameter analyzed for surface water in laboratory of environment and agriculture directorate in AL-Najaf Province.Six physicochemical parameter like Ec (mmho/cm),TDS(mg/liter),Ca+2(mg/liter),Cl-(mg/liter),So4-2(mg/liter),and PH.Standards Iraq for irrigation purpose were used and inverse distance waited(IDW) interpolation has been utilized to create of six parameters spatial distribution map above.Raster calculator of Arc map GIS 10.2 software presented water quality index for surface water samples ranged between 54.2912- 79.9387.

Breast carcinoma is the commonest malignancy in women and it has become a major health problem affecting women, worldwide. Mutations in the two breast cancer susceptibility genes, BRCA1/BRCA2 increase the risk of developing breast cancer.Objectives: To study the frequency of the three founder mutations; 185delAG and the 5382insC in BRCA1and the 6174delTin BRCA2 genes, using the Multiplex PCR Technique. Methods:In this study 150 patients with familial breast cancer diagnosed pathologically as having breast cancer collected from middle and south of Iraq from May 2017 to April 2018. In addition to control group(120 benign breast tumors) were used for dtection of BRCA1/BRCA2 mutations in the two groups.PCR amplification by using(KAPA 2GTMFast Multiplex PCR-Kit),was used for detection of mutation.Results: From all patients 34(22.7%) have BRCA mutations,16.7% patients with mutations were indicated to have one mutation in BRCA1"185 del AG or 5382 ins C" , 2.7% were found to have two mutations in BRCA1" 185 del AG and 5382 ins C",1.3% were verified to have one mutation "6174 del T" in BRCA2, 2.0% were indicated to have two mutations in both BRCA1 and BRCA2 "5382 ins C and6174delT",2.5%mutation were presented in control group.BRCA1"5382 ins C" mutation presented in 61.77% in ages less than 45 years,while BRCA2 "6174del T" was observed in 5.88% patients of ages higher 45 years. Conclusion: In Iraq, the frequency of breast cancer was presented in high percent in southern governorate than middle governorate due to They have the characteristics of significantly high frequency of family history,environmental factor high grade and advanced The BRCA1 (5382insC) mutations were percent than BRCA2.

يعمل التيفوئيد على ارتفاع مستويات الانزيمات والسايتوكينات الكبدية في حالات الالتهاب المزمن وخاصة عند الاطفال https://scholar.google.com/citations?view_op=list_works&hl=ar&user=vSmQeeoAAAAJ

تعمل عوامل الاكسدة والجذور الحرة المتكونة في جسم المرضى المصابين بالسرطان على تلف جميع مضادات الاكسدة وخاصة الانزيمات وفيتاميين C https://scholar.google.com/citations?view_op=list_works&hl=ar&user=vSmQeeoAAAAJ

ايض الكاربوهيدرات (عمليات الهدم والبناء) http://elearning3.uokufa.edu.iq/dent/course/view.php?id=19

طب اسنان عام/المرحلة الاولى /الكاربوهيدرات ,البروتينات والاحماض الامينية. طب اسنان عام/ المرحلة الثانية/ايض الكاربوهيدرات ,البروتينات والدهون.