مواقع التدريسيينجامعة الكوفة
Muslim Abd Ali Abdulhussein Alrubaye
Professor

Muslim Abd Ali Abdulhussein Alrubaye

0Publications
0Lectures

About

PhD in plant biotechnology ( in vitro mutagenesis) from Baghdad University (2005 ) , Head of Horticulture and landscape gardening Department , Faculty of Agriculture, University of Kufa (1996-1998). Member of Quality Assurance Unit, college of Agriculture, Kufa University (2008- 2012). Head of Horticulture and landscape gardening Department , Faculty of Agriculture, Kufa University from 2008- Yet. Participate in training courses in advanced plant tissue culture and sustainable agriculture in Italy.
Research interest
Plant biotechnology
Micropropagation
Fruit rootstocks improvement
In vitro mutation breeding
Plant physiology under stress
In vitro secondary product production
sustainable agriculture

Publications

9
2022

Effect of AgNps and different sources of Zinc on rooting and acclimation of strawberry (Fragaria ananassa Duch) cv. Rubygem in vitro

AuthorsHusham Khudhur, Muslim Abd Ali Abdulhussein
JournalKufa Journal For Agricultural Sciences – 2021:14(2): 1-13
Abstract

This study was conducted to examine the effect of adding different concentrations of nano silver nitrate (0, 5, 10 and 15 mg L-1) and zinc source {traditional(0.86mg L-1 as ZnSO4.7H2O) and nano zinc 0.086mg L-1 as Zinc oxide) to the specifies medium of rooting strawberry (Fragaria X ananassa Duch) cv. Rubygem, in addition to testing the efficiency of nano silver nitrate and zinc source on successful percentage of cultures adapting stage. Results revealed that highest rooting percentage, root numbers, roots length, leaves content of total chlorophyll and macro nutrients (N, P and K) were achieved the highest average at 10mg L-1 + nano zinc treatment. The source of cultures resulting from rooting stage affected the plant survival at acclimatization stage. The treatment 10 and 15mg L-1 of nano silver nitrate + nano zinc achieved 100% success percentage.

2022

Effects of AgNO3 in combination with some plant growth regulators on micropropagation of strawberry (Fragaria ananassa Duch)

AuthorsSuha Mohamed Nasir, Muslim Abd Ali Abdulhussein
JournalKufa Journal For Agricultural Sciences – 2022:14(1):33-40
Abstract

Strawberry (Fragaria ananassa Duch), belong to Rosaceae family, is widely appreciated,mainly for its fruit characteristic aroma, bright red color, juicy texture, It is traditionallyasexually propagated by rooted runners. To improve the strawberry varieties this method wasnot suitable due to incidence of many serious diseases infection resulting in the gradualdegeneration of cultivars performance, so micropropagated strawberry plant has beenintroduced to prevent most of the plant and soil transmissible diseases. Micropropagatedstrawberry has several advantages, such as its ability to multiply virus free stock rapidly and,the improved capacity of these plants to produce runners for planting in the field. In thisstudy, it was aimed to examine the interaction of BA, IBA and AgNO3 combinations atdifferent micropropagation stages. Results showed the addition of AgNO3 to MS medium ledto increase shoot number, shoot length, fresh and dry weight at multiplication shoots stage,shoots rooting % and root number at rooting stage. Survival plants %, plant height, leavesnumber and dry weight at acclimatization stage. (14) (PDF) Effects of AgNO3 in combination with some plant growth regulators on micropropagation of strawberry (Fragaria ananassa Duch). Available h_Effects_of_AgNO3_in_combination_with_some_plant_growth_regulators_on_micropropagation_of_strawberry_Fragaria_ananassa_Duch [accessed Feb 07 2023].

2019

EFFECTS OF PACLOBUTRAZOL AND SUCROSE IN DATE PALM (PHOENIX DACTYLIFERA L.) MICROPROPAGATION VIA DIRECT ORGANOGENESIS.

AuthorsHasaneen A. A. Awadh , Muslim Abd Ali Abdulhussein and Abdulminam Hussein Ali Almusawi
JournalPlant Archives Vol. 19 No. 1, pp. 1130-1134.
Abstract

The study was conducted in the Date palm micropropagation unit in vitro at College of Agriculture, University of Kufa during the period 2015 to 2016 to show the effect of the growth regulator (Paclobutrazol) that was added to the nutrient media at a concentration of (0, 5, 10, 15 mg.L-1) and its interaction with sucrose at a concentration of (30, 60, 80 g.L-1) in the program of micropropagation of date palms (Barhee cultivar) via direct organogenesis in the multiplication stage after culturing the quarters of the apical meristem. The study results showed that the biomass cultivated in the multiplication media (MS) that provided with 5 mg.L-1 Paclobutrazolrecorded the highest average number of buds (33.4 bud) and dry weight (1.050 g). The concentration of 10 mg.L-1 recorded an increase in the number of formed shoots (10.89 shoots), It did not have a significant effect on the fresh weight of the biomass. As for the effect of sucrose, the addition of (60 g.L-1) led to recording the highest average for the trait of number of formedshoots (17.67 shoot) and fresh and dry weight of biomass (6.56, 1.617g), respectively.While it did not have a significant effect in the number of multiplied buds.

2019

Impact of foliar application with dry yeast suspension and amino acid on vegetative growth, yield and quality characteristics of Olive (Olea europaea L.) Trees

AuthorsAli Hasan Ali, Mansoor Abed Aboohanah (2) Muslim Ali Abdulhussein
JournalKufa Journal For Agricultural Sciences – 2019:11(2):33-42
Abstract

Bashika Olive trees (Olea europaea L.) were sprayed twice at full bloom stage and one month after that one with dry yeast suspension (5, 10g.L-1) and amino acid (1, 2ml.L-1), alone and in combination besides control (spraying with water only) during 2017 and 2018 growing seasons. Results showed that foliar spraying of dry yeast suspension at 10g.L-1 with amino acid 2ml.L-1 gave the highest significant value of vegetative growth( leaf area, total chlorophyll leaf content) and foliar spraying of dry yeast suspension at 10g.L-1 with amino acid 1,2ml.l-1 gave the highest significant value of carbohydrate leaf content and carbohydrate shoot percentage in first season and dry yeast suspension at 10g.L-1 with amino acid 2ml.L-1 in second season. Also, results showed that dry yeast suspension at 5g.L-1with amino acid at 1ml.L-1 exhibited increase of fruit weight and fruit flesh weight in both seasons respectively. While, dry yeast suspension at 10g.L-1with amino acid at 1ml.L-1 treatment gave the highest significant average yield per tree-1in first and dry yeast suspension at 10g.L-1with amino acid at 0 ml.L-1 in second season. Treatment of dry yeast suspension at 10g.L-1with amino acid at 0,1and 2 ml.L-1 gave the highest significant value of fruit protein content in the first season and dry yeast suspension at 10g.L-1with amino acid at 1,2 ml.L-1 in seconed season, Dry yeast suspension at 10g.L-1with amino acid at 1,2 m.L-1 treatment gave the highest significant value of fruit oil content in both seasons respectively and highest value of percentage of unsaturated fatty acids in the first season

2018

Influence of Benzyladenine on Metabolic Changes in Different Rose Tissues

AuthorsMohammed Ibrahim, Xin Du, Manjree Agarwal, Giles Hardy, Muslim Abdulhussein and Yonglin Ren
JournalPlants 2018, 7(4), 95; https://doi.org/10.3390/plants7040095
Abstract

Two modern rose varieties, Floribunda and Hybrid Tea, were used to analyze and identify metabolic changes after foliar application with benzyladenine (BA). Volatile organic compounds (VOCs) as metabolites were detected. Two pairs of doses of BA, at 11.16 and 17.87 mg/cm2, and 7.17 and 12.26 mg/cm2 were applied to the foliage of Hybrid Tea and Floribunda, respectively. Sampling time was optimized and treatment duration was 4 weeks. After treatment, the volatiles from the treated and untreated control roses were extracted using headspace solid-phase microextraction (HS-SPME) technology by three-phase fiber 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) and analyzed by gas chromatography (GC) coupled with a flame ionization detector (FID), and with mass spectrometry (GC-MS).The results showed that BA and its dose rate led to metabolic changes of treated roses in comparison with untreated controls. The number of VOCs extracted and detected from leaves, stem, rhizosphere and whole plants from the two rose varieties at doses rate of 17.87 and 12.26 mg/cm2 were 43, 65, 40 and 68 compounds for each plant material, respectively, for both rose varieties. Whilst the VOCs extracted and detected from both rose varieties for leaves, stem, rhizosphere and whole plants were 38, 61, 34 and 66 compounds for each plant material, respectively. The results demonstrate that some volatiles, such as 4-Heptyn-2-ol, Phenyl methyl ether and 3-Methyl-apopinene, increased with increasing doses of BA; these compounds are aroma chemicals with a very powerful smell. This study shows that BA treatments can have a significant effect on metabolite changes in different rose tissues. This method could be applied to other floriculture plants.

2017

Salinity tolerance at seedling stage for rice genotypes: In vitro analysis

AuthorsRaghad Mouhamad, Lamiaa A Mutlag , Arif Nazir, Muslim Abd Ali Abdulhussein
JournalNet Journal of Agricultural Science
Abstract

Hydroponic cropping technique was utilized for evaluation of salinity tolerance of certain genotypes of rice under prevailing arid conditions of Iraq. The conditions envelop low level of characteristic issue, calcium carbonate conglomeration of soil and soils containing clear measures of gypsum, delicate to coordinate profile progression and low natural development. The seedling test is simple, rapid (4 to 6 weeks) and accurate. Furthermore, this test allows the screening of several seedlings under various experimental parameters. Therefore, the three genotypes of IRRI rice (IR71999-3R-3-2-2B-1-1, IR71829-3R-82-1-1and IR63731-1-3-3-2) and two varieties of rice (Anber and Jasmine) under salinity stress (1.2, 4, 8 and 10 dS/M) were investigated. The experiment was conducted in a growth chamber under simulated arid conditions in a completely randomized distribution. Salt stress caused reductions in rice plant production (yield) reached more than 30%. Dry matter weight was decreased by 24% in all varieties ofrice. Sodium (Na+) concentration was significantly (p ≤ 0.05) higher in NaCI-treated plants. The Na+/K+ and Na+/Mg2+ ratio in IRSSTN-SS2 (IR71999-3R-3-2-2B-1-1, IR71829-3R-82-1-1) genotypes and Anber plant indicate that it is more susceptible to salinity than the rice verities. Consequently, this cultivar is more vulnerable to saltiness than the assortment Cotaxtla, which demonstrated more noteworthy resistance to this anxiety

2015

Effect of Methyl Jasmonate and Ascorbic Acid on Growth of Troyer Citrange Rootstock Cultures under In Vitro Salt Stress

AuthorsMariam Basheer Esa, Muslim Abd Ali Abdulhussein
Journalمجلة الكوفة للعلوم الزراعية
Abstract

This experiment was conducted in Plant Tissue culture Laboratory / Horticulture and Landscape Department / Faculty of Agriculture /Kufa University during 2013-2014 .The aim was to study the effect of culture medium supplemented with NaCl salt, Methyl jasmonate and ascorbic acid on survival percentage, shoots number, its fresh and dry weights and its chlorophyll content of citrus rootstock Troyer citrange cultures under in vitro conditions . This experiment was carried as factorial using complete randomized Design with two factors, the first : NaCl at concentrations ( 0 , 75 or 150) mM and the second with combinations of (control , 2 mM of ascorbic acid ; 25 µM methyl jasmonate ; 50 µM methyl jasmonate ; 25 µM Methyl Jasmonate + 2 mM of ascorbic acid and 50 µM methyl jasmonate + 2 mM Ascorbic acid .) Results showed negative effects for NaCl on studied growth parameters of Troyer citrange rootstock cultures .It was found significant reduction in survival percentage, shoots number, its fresh and dry weights, and its chlorophyll content with an increase in NaCl levels in multiplication medium . They were the lowest values at 150 mM NaCl compared with the highest at control treatment ( 0 mM NaCl). Results also showed difference in responses of the plant growth regulators combinations in culture medium, treatment of 25 mM methyl jasmonate + 2 mM ascorbic acid increased significantly most studied parameters, compared with the free of plant growth regulators medium.

2014

Effects of Exogenous Putrescine Application on Hormone Content of Citrus aurantium L. Seedling under Salt Stress

AuthorsMuslim Abd Ali Abdulhussein
JournalJournal of Agricultural Science and Technology A 4 (2014) 521-528
Abstract

Abstract: A pot experiment was conducted in lath house to study the response of six month-old seedlings of sour orange (Citrus aurantium L.) irrigated with salinity water (1, 2 or 4 ds/m) for four month. The seedling was sprayed with putrescine at 0, 150 or 300 mg/L concentrations for three times with month interval, for investigation the effects of putrrescine spraying on hormonal changes in sour orange seedlings under salt stress. One month after the last putrescine application, the leaves of the vegetative shoots were picked for endogenous plant hormone determination. The results indicate that increasing level of irrigation water salinity to 4 ds/m significantly increased ABA, Put and SA; whereas, decreasing IAA and GA in leave. Spraying vegetative canopy with putrescine at 300 mg/L increased IAA, SA, ABA and Put, while decreased GA. It can be concluded that the adverse effects of high salinity irrigated water on endogenous plant hormone can be ameliorated, to some extent, by exogenous application of Put at 150 mg/L or 300 mg/L concentration.

2006

Evaluation of MM106 and Omara apple rootstocks for salt tolerance in vitro

Authorsا.م مسلم عبد علي الربيعي
Journalمجلة جامعة كربلاء
Abstract

This experiment was conducted at Plant Tissue Culture Laboratory / Agricultural and Biological Research Center / raqi Atomic Energy Commission during 2001-2003 to study the possibility of using in vitro shoot culture in rooting stage to evaluate salt tolerance of two apple rootstocks (MM106 and Omara).Single shoots were cultured on MS medium as a rooting media supplemented with 0, 20, 40, 60, 80 and 100mM NaCl for 8 weeks. All rooting parameters (rooting percentage, roots number, roots length )and plantlets growth(height of rooted shoot ) decreased as salt level increased in culture medium, with the reductions generally greater for Omara than MM106 rootstock. Also NaCl effect resulted in plantlet necrosis and a reduction in total chlorophyll content of both rootstocks.owever, plantlets of MM106 showed less relative root and shoot growth reduction under salt stress compared with Omara, therefore, it appeared to be more salt tolerant in vitro than Omara. Discover the world's research

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