مرتضى عبد المهدي محمد حسن المظفر

Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and significant decrease in blood vessel formation in treated tumour sections. The in vitro microarray analysis of 14 different angiogenesis factors revealed that NDV downregulated angiopoietin-1, angiopoietin-2, and epidermal growth factor in mammary adenocarcinoma cells. However, NDV elicited a different effect on Hep-2 as represented by the downregulation of inducible protein 10, intracellular adhesion molecule-1, and basic fibroblast growth factor beta in NDVinfected tumour cells. It was found out that microarray analysis results helped interpret the in vivo data. The results suggested that the NDV oncolytic strain reduced angiogenesis by interfering with angiogenesis factors that might reduce tumour cell proliferation, infiltration, and invasion.

Background and Aims :Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry. The virus belongs to the Avulavirus genus within the family Paramyxoviridae, subfamily Paramyxovirinae, of the order Mononegavirales and is designated avian paramyxovirus-1. So, Early detection of the virus by used sensitive and specific primer design for detect local outbreak isolate can prevent the spread of disease and avoid huge economic losses. Towards this goal, in this research, we developed reliable specific primers to reproduce specific amplification for NDV HN and M genes in One-step Real-time RT-Florescent PCR method. Materials and Methods: in this experimental study , Tow NDV isolate kindly provided by Iraqi center for cancer and medical genetic research , respective RNA was extracted from virus by using viral RNA/DNA extracted kit . the specific primers were designed according to NCBI data base for NDV HN and M gene sequence aligned by using ApE program and IDT company software Intercalating primer design website and sudsequently to amplify and evaluate by Real-time RT-Florescent PCR one step intercalaing dye . Results: the fourescent signle and the melting point analysis for amplification the NDV HN and M genes give positive results by specific primer design Conclusion: This study showed that the primer design for NDV HN and M genes are sensitive , specific and accurate by used Real-time RT-Florescent PCR Technique molecular assay for rapid diagnosis of local NDV outbreak isolate .

حضور مناقسة طالب الماجستير براء عقيل في قسم الاحياء المجهرية وعلى قاعة الدراسات العليا في كلية الطب البيطري وكانت المناقشة علنية والموسومة دراسة بعض الجواتب الجزيئية والمايكروبايولوجية لمتلازمة الرأس المنتفخ في دجاج اللحم حيث نال الطالب درجة الامتياز

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