Hasanat Abdulrazzaq Bakir Aljabery

Background: According to the last WHO report in 2014, Iraq is ranked as 22 out of 172 country in coronary artery disease (CAD) as the most leading cause of death. Iraq has an age standardized death rate of 187.65 due to CAD i.e., more than 187 per 100000 Iraqi people die due to CAD, which represent the first cause of death. To our knowledge, there is no Iraqi study concerning the association of MTHFR C677T gene polymorphism with sever stenosis.Objective: To study the link between C677T MTHFR gene polymorphism and coronary artery disease in individuals who had undergone coronary angiography in Iraqi people after we classify them into those with and without ischemia.Materials and Methods: population of the study comprised 150 patients (aged 50.4 ± 6.4 year) and 150 control subjects (aged 49.2 ± 4.6 year) that undergone to angiography for coronary vessels. We regarded angiography as positive when there is more than 70% reduction in the diameter of coronary vessels.Measurements of the participant's sugar and lipid profile were all carried out on fasting blood samples without anticoagulant by standard enzymatic assays. The MTHFR C677T polymorphism detection was carried out by PCR-RFLP method.Results: There is a significant statistical relationship between the MTHFR genotype and the presence of coronary artery disease (P< 0.001). In addition to statistically significant difference among the three genotypes CC, CT, TT regarding BMI, cholesterol level, triglycerides level, and VLDL (P < 0.05)Conclusion: MTHFR polymorphisms (CT, TT) genotype have been found to be risk factor for coronary artery disease in Iraqi population. Keywords coronary artery disease --- MTHFR C677T polymorphism --- coronary angiography --- Iraq

Background Globally, coronary artery disease (CAD) is considered to be the first major cause of death and estimated to be 8.1 million in 2013. Angina, myocardial infarction and heart failure represent the three most common forms of CAD. Adiponectin (ADN) is an adipokine predominately secreted from the adipose tissue. It has anti-inflammatory and anti-atherosclerotic properties. Several studies have suggested that adiponectin gene (ADN) single nucleotide polymorphisms (SNPs) is involved in the development of CAD, but with inconsistent results. Aim The aim of the present study is to highlight the association of rs2241766 SNP in exon2 of adiponectin gene with CAD in Iraqi population. Methods A case control study consisted of 150 patients with CAD attended the cardiology center in Najaf-Iraq and 150 healthy individuals. Selected patients were those of ≥70% narrowing of at least one part of the major coronary artery or its main branches on angiography. Patients and healthy individuals were genotyped for the +45T/G polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results The frequency of the genotypes of +45T/G SNP was significantly different in patients from those of the control group. The odds ratio of those of the GT genotype was found to be 2.01 (95% CI = 1.10–3.17, P = 0.019) and 6.67 (95% CI = 1.32–33.80, P = 0.022) for those of the GG genotype. The minor allele frequency G was found to be significantly elevated in patients (24.67%) relative to the control group (15.33%).These results suggested a risk factor to develop CAD of 2 and 6.67for carriers of the GT and GG genotypes respectively. Conclusion The rs2241766 (+45T/G) SNP of adiponectin gene is implicated in the pathogenesis of CAD in Iraqi population with a risk factor of 2 and 6.67 for carriers of the GT and GG genotypes respectively.