FOUAD ZAIDAN HAMZAH ALQURAISHI

The study's objective is to evaluate the enhanced effect of Ghrelin (GRE) on sperm analysis and the antioxidant system in male rats after inducing hyperthyroidism by L–thyroxin. The rats (50 males) were split into five groups. The first group consisted of 10 male rats that were given normal saline for 30 days S.C and set as a control group. While the second group(positive group) was 10 male rats with hyperthyroidism were given normal saline for 30 days S.C, the third group was 10 male rats with hyperthyroidism were given Ghrelin at a dose of (0.5nmol/100μl saline) for 30 days S.C, and the fourth group was male 10 rats with hyperthyroidism were given Ghrelin (1nmol/100μl saline) for 30 days S.C. The fifth group consisted of 10 hyperthyroid male rats that were given Ghrelin at a dose of (2nmol/100μl saline) for 30 days S.C. The results show a significant (P≤0.05) decrease in all parameters of sperm analysis , the number of births and antioxidative system in hyperthyroid group compared to control group. While, all treated groups with GRE (0.5, 1 and 2 nmol) shows enhance effect in sperm analysis, the number of births and antioxidant system compared to the hyperthyroid group.

Aim: The focus of this research was to see how the hormone ghrelin affected reproductive hormones and geneexpression of LH and Fsh hormone in mature male rats.Methods: Seventy male rats were used in the investigation, with twenty serving as controls and 50 being given asubcateaneous injection (s.c) of freshly manufactured thyroxine from (Sigma Aldrich Co.-USA) at a dose of 500μg/kg dissolved in saline to induce hyperthyroidism. For thirty days, After inducing hyperthyroidism, Thehyperthyroidic rats were divided into four groups at random. each with ten rats: a positive control group, ahyperthyroic group administrated with GRE at dose (0.5nmol/100μl saline), and a hyperthyroidic groupadministrated with administrated at dose (1nmol/100μl saline). The hyperthyroic group received GRE at a doseof 2nmol/100l saline. For thirty days,both the positive and negative control groups were given saline. (10μg/rat)Results: when compared to the control group, the hyperthyroidism group had a significant (P≤0.05) increase inserum (T, FSH, and LH) concentration, whereas when compared to the hyperthyroidism group, allhyperthyroidism groups treated with ghrelin had a significant (P≤0.05) decrease in serum (T, FSH, and LH)concentration. In the hyperthyroidic group, LH mRNA expression was considerably higher (p<0.0001) than inthe control group. When compared to the hyperthyroidic group, LH mRNA expression decreased considerably(p<0.0001) in all hyperthyroidic groups administrated with GRE. When comparing the hyperthyroidic group tothe control group, FSH mRNA expression increased considerably (p<0.0001). FSH mRNA expression, on theother hand, decreased considerably (p<0.0001) in all hyperthyroidic groups administrated with GRE. GRE hasbeen shown to have regulated effects on the reproductive hormones and expression of their related genes in adultmale rats.Conclusion: We discovered that GRE decreases the expression of the LH- and FSH hormones directly, whichcould be attributable to direct effects on the hypothalamusH-P-G. axis. ghrelin can regulator of the testicularfunctions and represents an important additional link between the male reproductive hormones homeostasis andthe reproductive function. (PDF) Effect of Ghrelin on Some Sexual Hormones and Molecular Characterization of Luteinizing Hormone and Follicular Stimulating Hormone Genes in Hyperthyroidic Male Rats. Available from: https://www.researchgate.net/publication/363250678_Effect_of_Ghrelin_on_Some_Sexual_Hormones_and_Molecular_Characterization_of_Luteinizing_Hormone_and_Follicular_Stimulating_Hormone_Genes_in_Hyperthyroidic_Male_Rats#fullTextFileContent [accessed Mar 21 2023].

The study's objective is to evaluate the enhanced effect of the ghrelin on the body weight and thyroid hormones in male rats after inducing hyperthyroidism by L-thyroxin. The rats (95 males) were split into two groups. The first group consisted of 25 male rats that were given normal saline for 30 days S.C and set as a control group. While the remainder of the animals were given levothyroxine 500g/kg subcutaneously for 30 days to induce hyperthyroidism. after induction the divided into 4 groups as followe ,the first one was the control group that mentioned previously, the second group was male rats were given normal saline for 30 days S.C, the third group was male rats were given ghrelin at a dose of (0.5nmol/100μl saline) for 30 days S.C, and the fourth group was male rats (1nmol/100μl saline) for 30 days S.C. The fifth group consisted of hyperthyroidism male rats that was given ghrelin at a dosage of (2nmol/100μl saline) for 30 days S.C. The results of final weight and weight gain are presented showed no significant difference in initial weight within all groups that were observed, while a significant decrease in final body weight in hyperthyroidism group compared with control group. On the other hand, the results revealed a significant decrease in body weight gain in male rats have hyperthyroidism compared with the control group. While the results observed a significant increase final body weight and body weight gain in all treated rats with ghrelin as compared with hyperthyroidism group. On other hand, the effect of hyperthyroidism on serum TSH, T3, and T4 concentrations revealed that the hyperthyroidism group had a significant rise in serum T3 and T4 concentrations when compared to the control group. While no significant drop in serum TSH concentration was observed in all hyperthyroidism groups handled with ghrelin (0.5, 1 and 2 nmol) as compared to the hyperthyroidism group, a significant decrease in serum T3 and T4 level was observed in all hyperthyroidism groups treated with ghrelin in comparison to the group of hyperthyroidism. Ghrelin peptide hormone and it has been shown to have potential effects on the body weight and thyroid hormone.

The main purpose of this research is to evaluate the high level of thyroxine affects on each of the luteinizing hormone (LH), testosterone, follicle-stimulating hormone (FSH) of rats and to estimate the potential effect of the administration of aqueous rosemary extract and propylthiouracil against testicular toxicity induced by levothyroxine in male rats. Negative control group rats were treated with distilled water. Three groups of male rats were treated subcutaneously with 0.5 mg/kg levothyroxine for 12 days: Since day 13th to the 24th day of the experiment first group was regarded as a positive control group that received distilled water, the second group was given propylthiouracil at a dose level of 10 mg/kg b.wt, and the third was given aqueous extracts of rosemary 10 mg/kg b.wt. The results revealed that treatment with aqueous extract of rosemary lead to a significant decrease in the levels of serum testosterone while a highly significant decrease in testosterone, FSH, and LH levels in serum revealed after treatment with propylthiouracil. Thus, it can be concluded that the effect of propylthiouracil and rosemary aqueous extract resulted in dialectical hormonal results in which the favor was to propylthiouracil.

The aim of the study to evaluate the enhanced effect of aqueous extract of rosemary on testis tissue in male rats after inducing hyperthyroidism by L-thyroxin and compare with propylthiouracil effect. The animals (32 male rats) were divided into two groups. The first group was a control group consists of 8 male rats that were treated with distilled water for 12 days and the rest of the animals induced with hyperthyroidism by levothyroxine 0.5 mg / kg subcutaneously for 12 days. After the induction, the animals were divided into four groups each consists of eight male rats. The first group was the control group and they were treated with distilled water for another 12 days, the second group included male rats induced hyperthyroidism were treated with distilled water for 12 days orally, while the third group included male rats induced hyperthyroidism that were treated with propylthiouracil at a dose of 10 mg / kg for 12 days orally, and the fourth group included male rats induced hyperthyroidism that were treated with rosemary extract at a dose of 12 mg / kg for 10 days, orally. The results showed that rosemary extract led to complete healing of testicular tissue, unlike propylthiouracil. … Read more

Here we carried out a comparative study of both propylthiouracil (PTU) and rosemary (Rosemarinus officinyroialis) to verify their effects on Aminotransferase (AST), and Alanine Aminotransferase (ALT) and Alkaline phosphatase (ALP) and lipid profile in Levothyroxine induced hyperthyroidism in rats. Four groups, each of 8 rats in group, were used in this study. Distilled water (DW) treated rats were considered as negative control group (C1). The three groups were treated with daily 0.5 mg/kg subcutaneous L4 for 24 days to induce hyperthyroidism. Since day 12th of the experiment DW, PTU 10 mg/kg and aqueous extracts of rosemary 10 mg/kg were orally administrated (C2, T1 and T2), once a day for 12 days. AST, ALT and ALP concentrations, Lipid profile levels and liver histopathology were examined to evaluate the effect of PTU and rosemary on liver function. There were significant increases in sera levels of ALT, AST and ALP in C2 in comparison with those of the C1. While, there were insignificant changes in serum levels of these enzymes in T1 and T2 in comparison to those in C1. On the other hand, L4 induced changes in serum lipid profile as compared to C1; while PTU shows no significant differences of serum lipid profile as compared with C1. The effect of rosemary shows less degree in recovering serum lipid profile comparing to PTU. It is concluded that PTU and rosemary have an ameliorative role on histopathological liver with variance effects on serum ALT, AST, ALP and lipid profile of male rats with hyperthyroidism. Keywords: lipid profile, oxidative stress, hyperthyroidism