فاطمة عبد الحسين مجبل التميمي

Candidosis or candidiasis is the most common fungal infection in the oral cavity and is caused by Candida species. The most common species in infected and healthy mouths is Candida albicans. During the period from September to November 2020, a total of 150 clinical samples were collected from Middle Euphrates Cancer Center in AL Najaf Governorate, which included swabs from the mouth. The Cultivation and identification methods of Candida albicans isolates were based on the morphological, cultural and biochemical characteristics, beside to the confirmative systems such as chromagar medium. And determine virulence factors (lipase , phospholipase and exopolysaccrid production)tube and in microtiter plate). Study the effect of fluconazole, alternative material (alcoholic extract curcumin&nanocurcumin ) on growth C. albicans and their virulence factors. This research seems to be, Fluconazole and nanocurcumin are affected on culture and virulence factors of Candida albicans while alcoholic extract curcumin are affected on virulence factors only.

Candidiasis is an acute or chronic infection produced by the Candida that is normally present in the mucous membranes of oral healthy individuals. During the period from September to November 2019, a total of 150 clinical samples were collected from AL_Zahraa Teaching Hospital and Middle Euphrates Cancer Center in AL_Najaf Governorate, which included swabs from the mouth through the swab (88 cancer samples, 62 thalassemia samples). The present study was conducted to isolation and identification Candida albicans from different clinical cases patients by different methods including direct examination, laboratory culture, finally, identification by PCR and determine virulence factors (adhesion and biofilm formation), determine biofilm by two method (in tube and in microtiter plate). Study the effect of antifungal, alternative material (isomethoxide) on growth C. albicans and their virulence factors. This research seems to be, Trbinafine and Fluconazole and isomethoxide are affected on culture and virulence factors of Candida albicans

Candidiasis is an acute or chronic infection produced by the Candida that is normally present in the mucous membranes of oral healthy individuals. During the period from September to November 2019, a total of 150 clinical samples were collected from AL_Zahraa Teaching Hospital and Middle Euphrates Cancer Center in AL_Najaf Governorate, which included swabs from the mouth through the swab (88 cancer samples, 62 thalassemia samples). The present study was conducted to isolation and identification Candida albicans from different clinical cases patients by different methods including direct examination, laboratory culture, finally, identification by PCR and determine virulence factors (adhesion and biofilm formation), determine biofilm by two method (in tube and in microtiter plate). Study the effect of antifungal, alternative material (isomethoxide) on growth C. albicans and their virulence factors. This research seems to be, Trbinafine and Fluconazole and isomethoxide are affected on culture and virulence factors of Candida albicans

This research was performed with 96 male and female patients aged from one to fifty years, divided into 7 β-thalassemia patients with HCV infection and 89 β-thalassemia patients without HCV infection, who visited the Thalassemia Center at Al- Zahra Teaching Hospital in Al–Najaf Iraq for disease management and 90 volunteer stable patients as a control group. Sample collection was conducted between April 2019 and the end of August 2019. This analysis tends to be 48 (48 percent) that offer Candida spp a good outcome. Sabouraud dextrose agar (SDA) was cultivated from all the collected samples. The good finding cultivated on Chromagar that contained most of the isolates was also Candida albicans 43.75%, led by Candida parapsilosis 39.58%, Candida galabrata 10.41%, Candida tropicalis 2.08% and 4.16% of unknow species. Some virulence factors in C.albicans such as: germ tube and the formation of biofilm were established in the present analysis. The findings showed that all C.albicans isolates were able to create germ tube and development of biofilms. Conclusion: There is a association between HCV- thalassemia patients that showed substantial difference at p- 0.05 between Infected and non- classes. Once cultivated on Chrom agar, the largest abundance of C. albicans than other animals

The present study was caied out to study effect of some Plant Extract against virulence factors of Candida albicans isolated from UTI patients, a total of 135 urine samples were collected from urinary tract infection patients. The isolation and identification methods of yeast isolates were followed upon the morphological, cultural and biochemical characteristics, in addition, the confirmative systems such as CHROMagar Candida and Api Candida were done for differentiation among Candida species. and determine the virulence factors phenotypic to C. albicanswhich involved (biofilm formation, phospholipase and germ tube). The results of this study show that C. albicansproduce all virulence factors in this research included phospholipase , germ tube and biofilm formation. The efficiency of some plant extract such as (clove, Eucalyptus and Casuarina) been evaluated to inhibition the growth of C. albicans as well as some virulence factors such as biofilm formation, germ tube and phospholipase, which were studied in this research. Statistically analysis results have been significance difference between the results of the substance concentrations and the concentrations of the different other substances. Candida albicans is the most causative agent of urogenital system infection in female and it is most common than male, cloveshows the highest effect against growth of Candida albicans and virulence factors, while casuarina appear moderate effect, but Eucalyptus didn’t appears any effect on Candida albicans.

Background: HSV-2 is thought to considered as the major sexual transmitted pathogen,which may cause severe complications is spontaneous abortion. Objective: This study was carried out to detect the rate of HSV-2 infection in women with spontaneous abortion by HSV-2 Ags ELISA kit. Materials and methods: Blood samples were collected from 200 pregnant women and 200women with spontaneous abortion. Blood specimens (5 ml) were taken from aborted women and controls under aseptic conditions and left for 10 minutes for spontaneous clotting at room temperature before being centrifuged (6000 rpm) to separate the serum. Serum samples were kept frozen at –20°C for determination of HSV-2 Abs. Results: The results showed that, this virus was detected in 30 (15%) out of aborted women and 18(9%) in pregnant women. The peak of infection level occurred in age group (20-29) years in both aborted and pregnant women. While in aborted women, the maximum positive HSV-2 cases were notices at the second trimester. Conclusions: Herpes Simplex -2 Virus may play a significant role in pregnancy loss. Its detection by sensitive ELISA techniques would permitinstantaneous therapeutic intrusion to raise the eventuality of a felicitous pregnancy.

الدكتور أحمد عبيس والأستاذ الدكتور فاضل الإبراهيمي على التقاعد، تقديراً لمسيرتهما العلمية وجهودهما الأكاديمية والإنسانية التي قدماها طوال فترة العطاء