First Report of the blaOXA-23 Gene in a Clinical Isolates of
Acinetobacter baumannii in Najaf Hospitals-Iraq
الباحث الأول:
Zuhair S. Alsehlawi
الباحثين الآخرين:
Jamal M. Alshara, Zainab J. Hadi and Ali M. Almohana
المجلة:
Research Journal of Microbiology, Academic Journals Inc.
تاريخ النشر:
None
مختصر البحث:
ABSTRACT
Carbapenemase producing Acinetobacter baumannii is frequently associated with nosocomial
infections. Increasing resistance to carbapenems, may significantly reduce the choice of effective
antibiotics. This study was conducted to determin…
ABSTRACT
Carbapenemase producing Acinetobacter baumannii is frequently associated with nosocomial
infections. Increasing resistance to carbapenems, may significantly reduce the choice of effective
antibiotics. This study was conducted to determine the occurrence of carbapenemase producing
A. baumannii isolates obtained from Najaf hospitals. Isolates were identified according to API
20NE system and more confirmed using 16Sr RNA gene. Carbapenem susceptibility was assayed
by microbroth dilution and other antibiotics using disks diffusion test. Phenotypic detection of
carbapenemase was performed using the imipenem-EDTA disk and modified Hodg tests. Then
isolates were subjected to monoplex PCR targeting blaOXA-23, blaIMP and blaVIM genes. Twelve
(1.5.%) A. baumannii isolates were recovered from clinical infections. Five (41.6%) of isolates were
found to be imipenem and meropenem resistant (MIC ranged 128-256 μg mLG1), of which, 4 (66.6%)
gave positive result with the imipenem-EDTA disk and modified Hodg test. PCR experiments
showed only two (33.3%) isolates were harbored blaOXA-23 gene. The present findings suggest that
emergence of OXA-23 carbapenemase-producing A. baumannii clinical isolates in Najaf hospitals.
