Susceptibility to Breast Cancer Based on XRCC3 Gene Polymorphism and Patient Profiles among Iraqi Women: A Case-Control Study.
الباحث الأول:
Zainab Sabah AL-Khalidi
الباحثين الآخرين:
Rand Muhammed Abdul-Hussein Al-Husseini
المجلة:
Medical Journal of Babylon
تاريخ النشر:
None
مختصر البحث:
Abstract
Background:
Among all cancers in women, breast cancer ranks first and accounts for 27% of malignancies; it is also the most common and deadly of these tumors. Multiple genetic variations, changes in DNA repair genes such as X-Ray Repair …
Abstract
Background:
Among all cancers in women, breast cancer ranks first and accounts for 27% of malignancies; it is also the most common and deadly of these tumors. Multiple genetic variations, changes in DNA repair genes such as X-Ray Repair Cross Complementing 3(XRCC3), and weakened DNA repair can all raise the chance of getting cancer. A high body mass index (BMI) and smoking are two lifestyle choices that put a person at a high risk for getting breast cancer. Women who are overweight or obese and smoke are extra possible to be diagnosed with breast cancer. The objective of this study is to inspect the possibility of an association between the XRCC3-Thr241Met gene polymorphism and some and demographic factors in patients with breast cancer. This study is the first study in Iraq that shed light on examining the effect of XRCC3-Thr241Met gene variants and its relationship to aging, body mass index, smoking status, and type of cancer in breast cancer patients.
Materials and Methods:
The blood of 75 Iraqi women who had been diagnosed with breast cancer was used in this study to extract DNA. In addition to using fifty blood samples from women who appeared to be healthy and without a family history of any other cancer as a control group. In the current study, information was collected either through a questionnaire which included age, body mass index calculation, and smoking status in women, or from hospital data such as type of cancer. Genotyping of XRCC3-Thr241Met gene was studied by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results:
The study noted a significant increase in the number of patients over the age of 50 years (p=0.008)
According to the BMI, this study found that overweight and obese women had a greater chance of getting breast cancer (37.3, 34.7%, respectively) compare with controls (p≤0.03). In terms of site of tumor origin, the most frequently occurring tumors were invasive ductal carcinoma (IDC) (69 cases, 92%) (P=0.0001). Strong associations have been found between risk of developing breast cancer and the XRCC3 241 (Met) allele (OR=3.3,95%CI=1.8-6.04; p=0.0001).
The results of this study showed that there was a statistically significant association(p=0.000002) between XRCC3 Thr241Met genotype polymorphism and BMI in women with breast cancer. The study reported that the Thr/Met +Met/Met genotypes were increased in obese and overweight women with breast cancer. The patients carrying the Thr/Met and Met/Met genotypes along with smoking and second handing smoking status were more susceptible to BC development (p-value =0.0001).the variant genotypes were more frequently seen in women with invasive ductal carcinoma (IDC) (p=0.039) than with invasive lobular carcinoma (ILC).
Conclusion: The mutant homozygous (Met/Met) genotypes of XRCC3-Thr241Met variants may be associated with the development and increase risk of the BC in Iraqi women. The mutant homozygous genotypes of XRCC3-Thr241Met variants may be associated with the development and increased risk of BC in Iraqi women. In addition, the mutant (Met) allele was significantly associated with the development of BC in women who were elderly, obese, overweight, smokers, and those affected by handing smoking and ICD type of cancer.
