Phenotypic and Genotypic Characterization of Some Virulence Factors in Streptococcus pneumoniae Isolated from Patients with LRTI in Najaf Province/Iraq
الباحث الأول:
Zahraa Yosif Motaweq
الباحثين الآخرين:
Habeeb S. NaherP**P, HawraaA.Ali.Al-DahhanP
المجلة:
International Journal of Scientific & Engineering Research, Volume 6, Issue 8, August-2015
تاريخ النشر:
None
مختصر البحث:
Abstract- This study provides genotypic data on 74 isolates of Streptococcus pneumoniae recovered from lower respiratory tract infections. In this study, some of them were detected by traditional phenotypic methods while others detected by phenotypi…
Abstract- This study provides genotypic data on 74 isolates of Streptococcus pneumoniae recovered from lower respiratory tract infections. In this study, some of them were detected by traditional phenotypic methods while others detected by phenotypic and then genotipically by using monoplex-PCR technique. The results showed that all (100%) isolates were encapsulated isolates. The molecular detection of cspA gene by using specific primer for S. pneumoniae revealed positive amplification for all isolates (100%). The results showed that overall, 89.2% of investigated isolates contained the lytA gene. In spite of, all (100%) of pneumococcus isolates have autolysin phenotypically. The results of this experiment indicated for positive amplification and it has been found that ply gene is found in 97.3% of pneumococcal isolates. In this study, the nanA and nanB genes were found in all S. pneumoniae isolates (100%) and (62.2%) respectively
