A harpin elicitor induces the expression of a coiled-coil nucleotide binding leucine rich
repeat (CC-NB-LRR) defense signaling gene and others functioning during defense to
parasitic nematodes
الباحث الأول:
Weasam A.R. Aljaafria, 2, 1, Brant T. McNeeceb, 1, Bisho R. Lawajua, Keshav Sharmab, Prakash M. Nirualab,
Shankar R. Pantb, 3, David H. Longc, Kathy S. Lawrenced, Gary W. Lawrencea, Vincent P. Klinkb, ∗
الباحثين الآخرين:
Weasam A.R. Aljaafria, 2, 1, Brant T. McNeeceb, 1, Bisho R. Lawajua, Keshav Sharmab, Prakash M. Nirualab,
Shankar R. Pantb, 3, David H. Longc, Kathy S. Lawrenced, Gary W. Lawrencea, Vincent P. Klinkb, ∗
المجلة:
Contents lists available at ScienceDirect
Plant Physiology and Biochemistry
journal homepage: www.
تاريخ النشر:
None
مختصر البحث:
A B S T R A C T
The bacterial effector harpin induces the transcription of the Arabidopsis thaliana NON-RACE SPECIFIC DISEASE
RESISTANCE 1/HARPIN INDUCED1 (NDR1/HIN1) coiled-coil nucleotide binding leucine rich repeat
(CC-NB-LRR) defense signalin…
A B S T R A C T
The bacterial effector harpin induces the transcription of the Arabidopsis thaliana NON-RACE SPECIFIC DISEASE
RESISTANCE 1/HARPIN INDUCED1 (NDR1/HIN1) coiled-coil nucleotide binding leucine rich repeat
(CC-NB-LRR) defense signaling gene. In Glycine max, Gm-NDR1-1 transcripts have been detected within root
cells undergoing a natural resistant reaction to parasitism by the syncytium-forming nematode Heterodera
glycines, functioning in the defense response. Expressing Gm-NDR1-1 in Gossypium hirsutum leads to resistance
to Meloidogyne incognita parasitism. In experiments presented here, the heterologous expression of Gm-NDR1-1
in G. hirsutum impairs Rotylenchulus reniformis parasitism. These results are consistent with the hypothesis that
Gm-NDR1-1 expression functions broadly in generating a defense response. To examine a possible relationship
with harpin, G. max plants topically treated with harpin result in induction of the transcription of Gm-NDR1-1.
The result indicates the topical treatment of plants with harpin, itself, may lead to impaired nematode parasitism.
Topical harpin treatments are shown to impair G. max parasitism by H. glycines, M. incognita and R. reniformis
and G. hirsutum parasitism by M. incognita and R. reniformis. How harpin could function in defense has
been examined in experiments showing it also induces transcription of G. max homologs of the proven defense
genes ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1), TGA2, galactinol synthase, reticuline oxidase, xyloglucan
endotransglycosylase/hydrolase, alpha soluble N-ethylmaleimide-sensitive fusion protein (α-SNAP) and serine
hydroxymethyltransferase (SHMT). In contrast, other defense genes are not directly transcriptionally activated
by harpin. The results indicate harpin induces pathogen associated molecular pattern (PAMP) triggered immunity
(PTI) and effector-triggered immunity (ETI) defense processes in the root, activating defense to parasitic
nematodes
