Molecular Diagnosis of Aminoglycoside Transferase Genes in
Methicillin - Resistant Staphylococcus aureus (MRSA) Strains
الباحث الأول:
Sumaya Najim Abedali Al-khateeb
الباحثين الآخرين:
Alaa Abdulhassein Rasool and Hashim Ali Abdualmeer Al-sherees
المجلة:
Indian Journal of Natural Sciences
تاريخ النشر:
None
مختصر البحث:
Resistance mechanism of aminoglycoside is the inactivation of enzyme action (aminoglycoside modifying
enzymes (AMEs) for example. aminoglycoside phosphotransferase, acetyltransferase and
nucleotidyltransferas) by the genes in plasmids or transposo…
Resistance mechanism of aminoglycoside is the inactivation of enzyme action (aminoglycoside modifying
enzymes (AMEs) for example. aminoglycoside phosphotransferase, acetyltransferase and
nucleotidyltransferas) by the genes in plasmids or transposons. aacA-aph Dcoded by aac(6′)gene, it is the
most commonly encountered AMEs in staphy.aureus. Other AMEs are aph(3”)-IIIa (phosphotransferases)
diffusion method. All of the genes were investigated by polymerase chain reaction (PCR) methods. Fifty
MRSA strains samples were determined by the polymerase chain reaction (PCR) methods, for the
detection of genes (three types): aaacA-aphD, aaadD andaaph (3”) - IIIa. The obtained results were
correlated genotyping with the susceptibility of the antibiotics (gentamicin, tobramicin, kanamicin,
neomicin, amikacin and netilmicin). aacA-aphD,aaaadD and aph(3”) – IIIa genes (positive strains) were
methicillin - resistant staphylococci. The aacA-aphDwas the most commonly detected.
