مختصر البحث:
Background: Ischemia/reperfusion (IR) injury is defined as
tissue ischemia with inadequate oxygen, followed by
reperfusion, initiating a wide and complex array of
inflammatory responses that may aggravate local injury and
induce impairment of re…
Background: Ischemia/reperfusion (IR) injury is defined as
tissue ischemia with inadequate oxygen, followed by
reperfusion, initiating a wide and complex array of
inflammatory responses that may aggravate local injury and
induce impairment of remote organ function by
mechanisms involving oxidative stress, inflammation, and
apoptosis.
Aim: The aim of this study was to investigate the potential
role of Zofenopril to improve myocardial I/R injury
induced by ligation of coronary artery in rats.
Materials & Methods: Adult male Albino rats were
randomized equally into four groups, harboring seven rats
each. Group I was the sham group: rats underwent the same
anesthetic and surgical procedure as the control group,
except for the ligation of the left anterior descending (LAD)
coronary artery; Group II was the control group: rats were
subjected to regional ischemia for 25 min by ligation of the
LAD coronary artery, followed by reperfusion for 2 hours;
Group III was the vehicle control group: rats received
normal saline intraperitoneally as a vehicle of Zofenopril,
subjected to regional ischemia for 25 min by ligation of the
LAD coronary artery, followed by reperfusion for 2 hours.
Group IV was the Zofenopril-treated group: rats were
pretreated with Zofenopril (15 mg/kg) intraperitoneally for
30 minutes before ligation of the LAD coronary artery. At
the end of each experiment, blood samples were collected,
using a direct cardiac puncture for the measurement of
plasma troponin T (cTnT), and serum MDA and GSH.
After blood sampling, the heart was dissected and divided
into two parts. The apex was used for histopathological
examination and the remaining part was used for the
measurement of cardiac tissue concentrations of TNF-α, IL-
6, IL-10, caspase-3 and Bax, after removal of the remaining
blood clots and aorta.
Results: In the active control group, compared with the
sham group, results showed that the myocardial tissue
concentrations of inflammatory cytokines TNF-α, IL-6,
IL-10, caspase-3, Bax, plasma cTnT concentration, and
serum MDA concentration were significantly increased (p <
0.05), while the serum GSH concentration was significantly
decreased (p < 0.05). Histopathologically, all rats in the
active control group showed a significant cardiac tissue
Rana Talib Al-Nafakh, Yasmeen Al 2 i Hussein, Najah R Hadi et al.
injury (p < 0.05) compared with the sham group. Zofenopril significantly prevented (p < 0.05) the increase of the myocardial tissue concentrations of TNF-α, IL-6, caspase-3 and Bax, Additionally, it counteracted the increase of the plasma cTnT concentration and serum MDA concentration; similarly, Zofenopril produced a significant increase (p < 0.05) in the cardiac tissue concentration of IL-10 and serum GSH concentration, and significantly reduced (p < 0.05) the cardiac tissue injury in the Zofenopril-pretreated rats.
Conclusions: The results show that Zofenopril improves myocardial I/R injury in rats by interfering with inflammatory reactions and apoptosis induced by I/R injury.
Keywords: Myocardial Ischemia/Reperfusion Injury, Zofenopril
