Biosynthesis of Silver Nanoparticles Produced from Penicillium chrysogenum against Klebsiella pneumoniae Isolated from Bacterial Vaginosis
الباحث الأول:
Noor K. Madlool
الباحثين الآخرين:
Raghad A. Sahib
المجلة:
Egyptian Journal of Medical Microbiology
تاريخ النشر:
1 يناير، 2026
مختصر البحث:
Bacterial Vaginosis (BV) has recently emerged as a global health issue especially in pregnant women because of its adverse outcomes. Various studies have shown the impact of BV on both mother and baby as well as overall reproductive health of women.…
Bacterial Vaginosis (BV) has recently emerged as a global health issue especially in pregnant women because of its adverse outcomes. Various studies have shown the impact of BV on both mother and baby as well as overall reproductive health of women. Objective: The aim of the study is To detect the effect of Silver Nanoparticles synthesized from Penicillium chrysogenum on Klebsiella pneumoniae isolated from patients with bacterial vaginosis. Methodology: 104 specimens collected from bacterial vaginosis patients from (September 2024 to February 2025) for isolation of Gram negative bacteria, to synthesis Silver Nanoparticles 5 mL of the fungal extract was added to the 100 mL of diluted silver nitrate solution. Results: Among the isolated Gram-negative pathogens, Klebsiella pneumoniae was the most prevalent species 21(20.19%), highlighting its significant role in infections associated with bacterial vaginosis and its resistance to multiple antibiotics. The effectiveness of silver Nanoparticles extracted and manufactured from the fungus, P. chrysogenum against Klebsiella pneumoniae, the Ultraviolet and visible spectroscopy (UV-Vis) was to confirm AgNPs which the UV-vis spectrum revealed the formation of wavelength peak for AgNPs is at (450nm) and absorption peaks at (1.6 nm), and also characterization by Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR) Analysis and X-Ray Diffraction Analysis (XRD). Conclusions: Silver nanoparticles produced by P. chrysogenum has high efficiency in inhibition, decreasing number and destroying the Klebsiella pneumoniae by AgNPs produced by this fungus at different concentrations (25, 50, and 100) μg / ml. The largest inhibition zone of AgNPs was (50 mm) by a concentration of (100 μg /ml), whereas the lowest inhibition zone was (12mm) at the concentration (25 μg/ ml).
