Association of IL-4 gene Polymorphism (rs2243250) and its Level as Genetic Risk Factor to Asthma in Iraqi Patients
الباحث الأول:
Qabas Abdulridha Abbas Hasnawi
الباحثين الآخرين:
Rand Muhammed Abdul-Hussein Al-Husseini
المجلة:
NeuroQuantology | June 2022 | Volume 20 | Issue 6 | Page 339-350 | doi: 10.14704/nq.2022.20.6.NQ2203
تاريخ النشر:
None
مختصر البحث:
Asthma is a chronic, complex respiratory disorder in which allergen-triggered inflammatory reactions in the airways contribute to the development of symptoms, including breathlessness, cough, wheezing, and dyspnea. It has been estimated that asthma …
Asthma is a chronic, complex respiratory disorder in which allergen-triggered inflammatory reactions in the airways contribute to the development of symptoms, including breathlessness, cough, wheezing, and dyspnea. It has been estimated that asthma affects about 300 million people in the world.Amplification refractory mutation system techniques (ARMS –PCR) was used for detection Interleukin-4(IL-4) gene polymorphism -590 C>T (rs2243250). The genotype distribution results of the IL-4 gene showed significant differences p<0.05 between controls (CC: n=19, 95%; CT: n=1, 5%; TT:n=0) and asthma patients (CC: n=35, 50%; CT: n=33, 47.14 %; TT: n=2, 2.86%). The result showed an increase in CT, TT genotype, and T allele of -590 C>T in asthma patients than in controls, and they were significantly more likely than in controls to have the mutant allele (OR=14.010, 95%CI= 1.858 -105.627, p=0.01).Results revealed that serum IL-4 levels were significantly higher (P<0.05) in patients (9.65±0.27pg/ml) compared with the healthy group (5.47±0.32 pg/ml).The result showed the levels of the IL-4 recorded a significant increase (P<0.05) in the patients (9.65±0.27pg/ml) compared with the healthy group (5.47±0.32pg/ml). The study showed that theIL-4 genotype variants are indicative of IL-4 cytokineconcentrations, with the highest levels in asthma patients with CC variants (9.37±0.35pg/ml) compared to the homozygous CC genotype of controls (5.39±0.32 pg/ml). The heterozygote CT and variants of homozygote TT significantly had higher IL-4 cytokine levels in patients (9.88±0.02pg/ml&10.87±1.11pg/ml, respectively) compared to the IL-4 CCvariant (9.37±0.35pg/ml) in patients, and heterozygote CT in controls had higher IL-4 cytokine levels (7.16±0.0 pg/ml) compared to IL-4 CCvariant (5.39±0.32pg/ml) in controls. These results imply an association between the polymorphism of the IL-4 -590 C>T and IL-4 cytokine levels in the Iraqi asthma patient.
