CARACTERIZAÇÃO ANTIBACTERIANA DE NANOPARTÍCULAS DE TITÂNIO
NANOSSINTETIZADAS POR STREPTOCOCCUS THERMOPHILUS
الباحث الأول:
ALDUJAILI, Nawfal Hussein*
الباحثين الآخرين:
BANOON, Shaima Rabeeh
المجلة:
PERIÓDICO TCHÊ QUÍMICA
تاريخ النشر:
None
مختصر البحث:
Metals that contain nanomaterials have the potential to be employed in controlling different kinds of
infection, however, very limited information is known about their antibacterial properties. This study has been
done to investigate the nanosynt…
Metals that contain nanomaterials have the potential to be employed in controlling different kinds of
infection, however, very limited information is known about their antibacterial properties. This study has been
done to investigate the nanosynthesis titanium nanoparticles (TiNPs) using Streptococcus thermophilus and
weighing their biological actions as antibacterial. The bacterial isolates identified using universal primers 16S
rRNA; then The 16S rRNA gene nucleotide sequences were aligned with the nucleotide sequences of strains
obtained from the GeneBank through the software CLUSTAL X (version 1.82). Titanium nanoparticles were
nanosynthesized by adding 0.025M titanium dioxide (TiO2) into cell-free supernatant for Streptococcus
thermophilus. TiO2 was used as a precursor for nanobiosynthesis TiNPs. The formation of TiNPs was indicated
by the color alteration of the solution from the light brown into dark brown indicates for the production of TiNPs.
The Characterization of nanobiosynthesis was accomplished with UV-Visible (absorbance at 377nm), Scanning
Electron Microscope, X-ray diffraction, Atomic Force Microscope, Energy-dispersive X-ray spectroscopy was
used to distinguish the dimension, form (spherical) by SEM, dispersal (homogenous) and elemental analysis of
nanoparticles. Biogenic TiNPs have displayed antibacterial and antibiofilm activity against both Multidrugresistant
Klebseilla pneumonia and Staphylococcus aureus. As an antibacterial activity, the TiNPs inhibited
significantly K.pneumoniae (20mm) with concentration (500μg/ml), and S. aureus (16mm) with the same
concentration and increasing the concentration of TiNPs the inhibition zone increased. While as antibiofilm
activity of TiNPs using the tube method, the tubes containing bacterial suspension K.pneumoniae and S.aureus
with TiNPs, the results showed biofilm formation was prevented and removed by the effect of TiNPs.
