Vitamin E and telmisartan attenuates doxorubicin induced cardiac injury in rat through down regulation of inflammatory response
الباحث الأول:
Najah Hadi2
الباحثين الآخرين:
Nasser Ghaly Yousif1*, Fadhil G Al-amran2, Nadhem K Huntei2, Bassim I Mohammad3 and Sadiq J Ali2
المجلة:
BMC Cardiovascular Disorders
تاريخ النشر:
None
مختصر البحث:
The importance of doxorubicin (Dox), as a potent antitumor antibiotic, is limited by the developmentof life-threatening cardiomyopathy. It has been shown that free radicals are involved in acute doxorubicin-inducedtoxicity. The aim of this study wa…
The importance of doxorubicin (Dox), as a potent antitumor antibiotic, is limited by the developmentof life-threatening cardiomyopathy. It has been shown that free radicals are involved in acute doxorubicin-inducedtoxicity. The aim of this study was to determine the protective effect of vitamin E and telmisartan in acutedoxorubicin induced cardiotoxicity.Methods: Thirty two male Sprague - Dawly rats were involved in this study and were randomly separated into 4groups, eight rats in each group, one group received normal saline I.P as control and second group receiveddoxorubicin 20 mg/kg I.P, the other two groups also received doxorubicin 20 mg/kg I.P as single dose after sevencumulative doses (for seven days) of vitamin E (100 mg/kg) and telmisartan (1 mg/kg) respectively.Immunofluorescent staining for monocytes infiltration and analyses of plasma by (ELISAs) for MCP-1and troponin I.Western immunoblotting assay for ICAM-1, while left ventricular function was analyzed by microcatheter, alsoestimated the level of oxidative stress parameters (MDA and Catalase) and cardiac enzymes activities (CK-MB andLDH) before starting drugs treatment and after treatment period by 48 hours.Results: The immunofluorescent staining showed that administration of vitamin E and telmisartan are attenuatedof mononuclear cell infiltration; (p < 0.05 vs. Dox group), also reduced the level of chemokines MCP-1 and ICAM-1expression compared with Dox group only, and there is marked reduction of myocardial troponin-I levels withimproved LV function in vitamin E and telmisartan treated group. Doxorubicin treatment increased MDA, LDH,CK-MB levels significantly (P < 0.01), and were counteracted by administration of vitamin E and telmisartan, but didnot significantly affect serum catalase activity.Conclusions: Antioxidant effect (Vitamin E and telmisartan) have been shown to decreasedoxorubicininduced cardiotoxicity.
