Detection of Clinical and Subclinical Foot and Mouth Disease Virus in Cattle in Al-Najaf Province
الباحث الأول:
Abd Hatem
الباحثين الآخرين:
Ahmed Abdul Wahid Al Anbagi, N1, Al-Alo, K. Z. K1,
المجلة:
Archives of Razi Institute, Vol. 77, No. 3 (2022) 1185-1189 Copyright © 2022 by
تاريخ النشر:
None
مختصر البحث:
Abstract
Foot-and-mouth disease (FMD) is a highly transmissible disease caused by Aphthovirus of the family Picornaviridae. This study aimed
to investigate the serological approach (non-structural protein [NSP] analysis) of 3ABC enzyme-linked immu…
Abstract
Foot-and-mouth disease (FMD) is a highly transmissible disease caused by Aphthovirus of the family Picornaviridae. This study aimed
to investigate the serological approach (non-structural protein [NSP] analysis) of 3ABC enzyme-linked immunosorbent assay (ELISA)
to diagnose FMD cattle in vaccinated and unvaccinated animals. A total of 84 serum specimens, including non-vaccinated, singlevaccinated,
and multi-vaccinated samples, were collected from four districts in Baghdad Province, Iraq, to evaluate the antibodies to
NSP of the FMD virus. The ELISA was used to detect antibodies (NSP) of FMDV in the serum of cattle. The result showed that the
seroprevalence was estimated at 34% (29/84) in farm animals. The seroprevalence rates of FMD in relation to the age of infected animals
were obtained at 21%, 7%, and 6% in 9-23-, 24-36-, and ≥ 36-month-old groups, respectively. The consequences of the examination of
the sera from naive, immunized, and non-immunized infected farm animals applying 3ABC-ELISA were presented; accordingly, the
incidence rates of FMD infection in non-vaccinated and vaccinated animals were 18 (75%) and 11 (18%) respectively. Negative results
were recorded in the immunized group 49 (82%) higher than in the non-immunized group 6 (25%). Evaluation of NSP antibodies to
isolate vaccinated animals from infected ones showed that the application of these assays was significantly useful for FMD prevention
and control management programs in infected areas.
Keywords: Foot and mouth disease virus, Non-structural
