Activity and stability of urease enzyme immobilized on Amberlite resin
الباحث الأول:
Jawad Kadhim Jawad AL-SHAMS, Mustafa Abdul Kadhim HUSSEIN*, and Hussein Kadhem ALHAKEIM
الباحثين الآخرين:
Jawad Kadhim Jawad AL-SHAMS, Mustafa Abdul Kadhim HUSSEIN*, and Hussein Kadhem ALHAKEIM
المجلة:
Ovidius University Annals of Chemistry Volume 31, Number 1, pp. 1 - 4, 2020
تاريخ النشر:
None
مختصر البحث:
mmobilization of enzymes is a good field of study to extend the life of enzyme and reduce the cost of the
chemical processes, such as separation processes. Urease is an important enzyme with medical and industrial applications.
The aim of the pres…
mmobilization of enzymes is a good field of study to extend the life of enzyme and reduce the cost of the
chemical processes, such as separation processes. Urease is an important enzyme with medical and industrial applications.
The aim of the present study is to prepare an immobilized urease on a strong cation exchange resin (Amberlite IR120 Na)
and study its activity and stability. We monitored the release of Na ions in the collected fractions and searching for enzyme
in the fractions as indicators of immobilization by ion exchange phenomenon. Sodium is determined by using atomic
absorption spectroscopy technique, while the enzyme concentration was tested by Bradford’s method. Immobilized urease
activity was evaluated by salicylate-hypochlorite method. The results indicated a complete immobilization of urease
enzyme on the resin surface with reserving 92% of the activity of free enzyme. The immobilized urease enzyme on resin
showed good stability and it has a 62% of its activity after 154 days of storage at room temperature. It is concluded that
a new immobilized urease enzyme system is prepared with good enzyme activity and stability.
