NEWCASTLE DISEASE VIRUS SUPPRESSES ANGIOGENESIS
IN MAMMARY ADENOCARCINOMA MODELS
الباحث الأول:
Ahmed Majeed
الباحثين الآخرين:
Murtatha Abdulmahdi mohammed
المجلة:
Bulgarian Journal of Veterinary Medicine, 2020 ONLINE FIRST
تاريخ النشر:
None
مختصر البحث:
Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth
and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of
Newcastle disease virus (NDV) is an RNA vir…
Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth
and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of
Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour
cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic
activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested
against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo
antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour
sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal
carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism
of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant
tumour regression and significant decrease in blood vessel formation in treated tumour sections. The
in vitro microarray analysis of 14 different angiogenesis factors revealed that NDV downregulated
angiopoietin-1, angiopoietin-2, and epidermal growth factor in mammary adenocarcinoma cells.
However, NDV elicited a different effect on Hep-2 as represented by the downregulation of inducible
protein 10, intracellular adhesion molecule-1, and basic fibroblast growth factor beta in NDVinfected
tumour cells. It was found out that microarray analysis results helped interpret the in vivo
data. The results suggested that the NDV oncolytic strain reduced angiogenesis by interfering with
angiogenesis factors that might reduce tumour cell proliferation, infiltration, and invasion.
