VALIDATION GENE EXPRESSION OF RNA-BINDING PROTEINS AND BIOMARKERS IN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCER
الباحث الأول:
Muneer Khudhair
الباحثين الآخرين:
Maha Fadhil Smaism
Mohammed Ridha Joodi A.Jalo
المجلة:
Turkish Journal of Physiotherapy and Rehabilitation
تاريخ النشر:
None
مختصر البحث:
Prostate cancer is the most common cancer in the world specifically in Iraq, compared to other cancers, that
affects men of old ages of 50 years and more, but it rarely affects men that lesser than this age category. Benign
prostate hyperplasia (B…
Prostate cancer is the most common cancer in the world specifically in Iraq, compared to other cancers, that
affects men of old ages of 50 years and more, but it rarely affects men that lesser than this age category. Benign
prostate hyperplasia (B.P.H) is not cancer and does not develop into cancer. But it can be a serious medical
problem for some men. Benign prostatic hyperplasia (BPH) is a histologic diagnosis that refers to the
proliferation of smooth muscle and epithelial cells. The current study included extracting RNA from white
blood cells and then converting it to cDNA. The gene expression of the Sam 68 was estimated in patients with
Prostate Cancer and Benign Prostate Hyperplasia using Real time-PCR. The thesis also included the estimation
of the studied chemical parameters by ELISA, which are: AKT1, B- catenin . The purity and concentration of
RNA was estimated in two ways: using two techniques, the first is Nano Photometer, where the concentration
was 55 ±13, and another technique called Quantus Fluorometer, and the concentration was 58 ±4.2. The current
study proved that the sam68 gene was high gene expression in prostate cancer, where were highly significant
P˂0.001, when compared to the gene expression in healthy people by using real time- pcr in presence the house
keeping gene. Also, the gene expression was higher at the greater the age of the patient, as it was of high
significance P˂0.001 whenever the age of the patient with prostate cancer was greater than 50 years, while the
gene expression was of less significance when the patient is less than 50 years old, but in a lesser degree.There
was no statistical significance in gene expression between benign prostate patients compared with healthy
people, where a p value of more than 0.001. The studied chemical markers was estimated in the serum of
patients with prostate tumors (60 with PCa and 60 BPH), using the ELISA technique. The current study showed
that the enzyme AKT1 was highly activity in prostate cancer and benign prostate patients when compared with
the it’s activity in healthy people and was of high significance, as it recorded a P <0.001. Statistical results of
the Beta-catenin protein were highly significant in patients with prostate cancer and BPH and the value of P
<0.001
