In vitro evaluation of the protoscolicidal effect of Eucalyptus globulus essential oil on protoscolices of hydatid cyst compared with hypertonic saline, povidone iodine and silver nitrate (Journal of Visceral Surgery
الباحث الأول:
M. Moazeni a,
الباحثين الآخرين:
S.V. Hosseini b, M.H. Al-Qanbar c,
A.M. Alavi a, H. Khazrae
المجلة:
Journal of visceral surgery
تاريخ النشر:
None
مختصر البحث:
Summary
Objective: There are various protoscolicidal agents for inactivation of protoscoleces of hydatid
cysts before and during surgical operation. The present study was aimed to evaluate the protoscolicidal effect of two concentrations of Eucaly…
Summary
Objective: There are various protoscolicidal agents for inactivation of protoscoleces of hydatid
cysts before and during surgical operation. The present study was aimed to evaluate the protoscolicidal effect of two concentrations of Eucalyptus globulus on protoscoleces of Echinococcus
granulosus sensu lato under in vitro condition and to compare its efficacy with hypertonic saline,
povidone iodine and silver nitrate.
Methods: Live protoscoleces obtained from the liver of naturally infected sheep were exposed
to 0.5% and 1% of Eucalyptus globulus essential oil, 5% hypertonic saline, 10% povidone iodine
and 0.5% silver nitrate for 1 and 3 minutes. Phosphate buffered saline was used as a negative
control. One percent eosin staining method was used to test the viability of protoscoleces in
different groups.
Results: While the mean percentage of dead protoscoleces was 6.08% in the control group, the
scolicidal power of 5% hypertonic saline was only 6.54% and 6.60% after 1 and 3 min respectively. 0.5% E. globulus EO demonstrated 97.38% and 100% scolicidal activity after 1 and 3 min
respectively. The mean protoscolicidal power of 1% E. globulus EO, 10% povidone iodine and
0.5% silver nitrate was 100% after one minute.
Conclusions: According to the results of this study, E. globulus EO demonstrated high scolicidal
power in a short period of time. Hence, this herbal product could be considered as a potent
natural scolicidal agent that could be used before and during surgery of hydatid disease.
© 2019 Published by Elsevier Masson SAS.
