نظام مواقع التدريسيين

Bacterial Infection, IL-17 and Some Biomarkers with Related to Covid-19 Patients’ Outcome

الباحث الأول: Mayyada F. DARWEESH1

الباحثين الآخرين: وSddiq Ghani AL-MUHANNA2 وSaad Abdulhussein ABD1

المجلة: Latin American Journal of Pharmacy )

تاريخ النشر: None

مختصر البحث:

SUMMARY. Since it has been a global pandemic for the past three years, the coronavirus disease 2019, or COVID19, has spread to all parts of the world. It first appears as pneumonia, which can progress to severe respiratory failure and has asits ma… SUMMARY. Since it has been a global pandemic for the past three years, the coronavirus disease 2019, or COVID19, has spread to all parts of the world. It first appears as pneumonia, which can progress to severe respiratory failure and has asits major hallmark a systemic inflammatory immune response brought on by an increased production of cytokinesthat causes a cytokine storm. Bacterial co-infections raise the risk of morbidity and mortality in COVID19 patients. The current study was conducted to investigate the impact of bacterial co-infection, IL-17, D-dimer, ferritin and CRP in Covid-19 pneumonia outcome. Four mL of blood samples were collected from 120 patients attending to AL-Amal hospital and Al-Sader medical city, Najaf/Iraq, from July 2021 - January 2022; 1.5 mL of blood kept in tube containing 3.2% sodium citrate to estimation D-dimer by Mini-vides and 2.5 mL in plane tube to separate serum that used to detect IL-17, ferritin and CRP. Patient divided into Critical 33 (27.5%), sever 42 (35%) and Mild/Moderate (M/M) 45 (37.5%), In addition to 60 apparently healthy subjects as controls group. The result indicated that a significant increase (p < 0.05) in mean serum level ofIL-17 in patients compare to healthy group, and the mean critical and server cases higher than M/M cases (101.79, 74.83, and 27.65) pg/mL. The results founded that most bacterial co-infection within critical and sever cases with 22(44%) and 26 (52%) respectively while M/M cases were 2(4%) only. Finally the results founded an elevated number of leukocyte, higher neutrophil and higher infection-related biomarkers (S. ferritin, D-dimer, and CRP) especially in critical cases (18.5± 2.62, 88.42±12.6, 738.68±154.41, 5.76±2.75, and 122.85±35.39) respectively.In conclusion the level ofIL-17, ferritin and D-dimer highly increased in Covid patients that correlated with severity of Covid pneumonia so by this biomarkers can recognized between two types of patients. Secondary bacterial infection increased progressive state of patients.

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The Immune-modulator Activity of Pseudomonas aeruginosa Extracted Protein: Azurin Charming Protein

الباحث الأول: Lina A. HASSAN1

الباحثين الآخرين: Mayyada F. DARWEESH1 & Thualfakar Hayder HASAN

المجلة: Latin American Journal of Pharmacy

تاريخ النشر: None

مختصر البحث:

SUMMARY. Vaccines regarded as one of the most successful medical interventions to date, some microorganisms are capable to acting as immunomodulation agents one of them Pseudomonas aeruginosa produce secondary metabolite. Azurin a bacterial protei…

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Biofilm Formation Frequency and Drugs Resistance of Staphylococcus Aureus Isolated From Clinical Samples

الباحث الأول: Mayada Farhan Darweesh

الباحثين الآخرين: Zahraa Abdulrazaq Al-Khfaji

المجلة: Published by AIP Publishing.

تاريخ النشر: None

مختصر البحث:

The prevalence of Extensively Drug-Resistant (XDR) , Multidrug-Resistant (MDR) and Pan-drug-Resistant (PDR) phenotypes amongst S. aureus strains have become one of the greatest challenges. This study aimed to determine the association of XDR, MDR,…

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The Role of IL-12 in the Aetiology of SLE and its Connection to HBV Infection in Iraqi Patients

الباحث الأول: Lina A Hassan1

الباحثين الآخرين: Mayyada F Darweesh

المجلة: T J Commun Dis. .

تاريخ النشر: None

مختصر البحث:

Introduction: Systemic lupus erythematosus (SLE) is a significant social and public health issue because the medications used to treat it can only manage the symptoms and halt the progression of the disease, not totally cure it. The purpose of…

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The impact of bacterial prostatitis and IL-17 in male infertility

الباحث الأول: ALhadrawi Kais Khudhair1,

الباحثين الآخرين: Darweesh Mayyada

المجلة: Res.J.of Biotechnology

تاريخ النشر: None

مختصر البحث:

Abstract A case-control study aimed to assess the role of IL-17 serum level and bacterial prostatitis in development of male infertility. 120 patients from January to June 2021 included 60 prostatitis infertile patients, 30 prostatitis patients and…

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The Impact of IL-35, Bacterial Prostatitis in Development Male Infertility in Najaf Province Patients

الباحث الأول: Kais Khudhair Al-hadrawi

الباحثين الآخرين: Mayyada F. Darweesh

المجلة: The Egyptian Journal of Hospital Medicine (October 2022)

تاريخ النشر: None

مختصر البحث:

ABSTRACT Background: A case-control study examined the relationship between bacterial prostatitis and IL-35 blood levels in the emergence of male infertility. Objective: This study aimed to assess the role that bacterial prostatitis and IL-35 play…

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Evaluate microbial profile and IL-17 level in transmission transfusion infection patients

الباحث الأول: Mayyada F Darweesh

الباحثين الآخرين: Murtadha Rahi Jabbar

المجلة: International Journal of Health Sciences,

تاريخ النشر: None

مختصر البحث:

Abstract---Transfusion-transmitted infections (TTIs) an important causes of morbidity and mortality among recipients of blood /blood products since it among the essential vehicles for infectious agents that lead to disability or death. So this…

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Evaluate IL-10 level and gene polymorphism in β-Thalassemia patients with related to microbial infection and disease outcome

الباحث الأول: Mayyada F. Darweesh

الباحثين الآخرين: Murtadha Rahi Jabbar

المجلة: International Journal of Health Sciences,

تاريخ النشر: None

مختصر البحث:

Thalassemia is a genetic blood disorder inherited from the parent with unusual production of hemoglobin, infectious complications, immune abnormalities and iron overload with related to organ damage that consider a chief cause of morbidity and…

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Evaluate IL-10 level and gene polymorphism in β-Thalassemia patients with related to microbial infection and disease outcome

الباحث الأول: Mayyada F. Darweesh

الباحثين الآخرين: Murtadha Rahi Jabbar

المجلة: International Journal of Health Sciences,

تاريخ النشر: None

مختصر البحث:

Thalassemia is a genetic blood disorder inherited from the parent with unusual production of hemoglobin, infectious complications, immune abnormalities and iron overload with related to organ damage that consider a chief cause of morbidity and…

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Antibacterial effect of Cappariloside (C22H28N2O11) and Vitexlactam (C22H35NO4) alkaloids compounds isolation from Capparis spinosa roots & Vitex agnus-castus fruits.

الباحث الأول: Dr. Ahmad Abies

الباحثين الآخرين: Dr. Mayada Farahan و Diana Basim Abdul hameed

المجلة: Al-Kufa University Journal for Biology/ Print ISSN: 2073-8854 & Online ISSN: 2311-6544

تاريخ النشر: None

مختصر البحث:

crystal yellowish white powder. The chemical and physical properties of isolated alkaloids compounds were studied by using thin layer chromatography (TLC), melting point (MP), ultraviolet spectrum (UV) and FTIR spectrum. The isolated alkaloids compo…

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Assesment of capslar antigen extracred from Proteus volgaris as immune stimulater and antibacterial activity

الباحث الأول: D .Mayada F.Darweesh

الباحثين الآخرين: nebras yuheaa , Nofel husean Al-dujelea

المجلة: Print ISSN: 2073-8854 & Online ISSN: 2311-6544 Magazin of Al-Kufa University for Biology

تاريخ النشر: None

مختصر البحث:

The current study including two parts : The first was determination antimicrobial effect of Proteus vulgaris on two type of pathogenic bacteria such as S.aureus and P. aeruginosa , the second was evaluate the effect of capsular polysaccharide antige…

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The role of antioxidants protein in regulated thalassemia patients state in relation with humeral immune system

الباحث الأول: mayyada F.Darweesh

الباحثين الآخرين: D.Wafaa S.ALwazni D.Fatema A.MajbeelP

المجلة: International Journal of Scientific & Engineering Research, ISSN 2229-5518

تاريخ النشر: None

مختصر البحث:

Abstract_This study was designed to determine the role of interaction between humeral immune system and antioxidants protein in regulation thalassemic patient statement. Seventy tow blood samples from patient admitted to the regulation thalassemia …

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The antibacterial & antibiofilm activity of Punica granatum peel aqueous extract against some oral pathogen

الباحث الأول: Dr.wafaa S. Al-wazni

الباحثين الآخرين: اSend Al-dwri و Dr. Mayyada F. Darwees

المجلة: Print ISSN: 2073-8854 & Online ISSN: 2311-6544 Magazin of Al-Kufa University for Biology

تاريخ النشر: None

مختصر البحث:

This study was designed to evaluate the antibacterial & antibiofilm activity of Punica granatum peel aqueous extract against oral pathogens from patients suffering from gingivitis and dental carrier. These isolates were 58.8% gram positive (Lactobac…

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The Role of Interferon- Gene Polymorphism in Spontaneous Abortion

الباحث الأول: Fatima A. AL-Timimi,

الباحثين الآخرين: Mayyada F. Darweesh, Ali R. Hussein

المجلة: International Journal of Scientific & Engineering Research, Volume 5, Issue 12, December-2014 ISSN

تاريخ النشر: None

مختصر البحث:

20-22 weeks of pregnancy causing dangers to the mother and her fetus. In recent years, a lot of studies suggest that recurrent abortion is caused by the genetic factors. Aim: The aim of this study was to investigate the association of the IFN- +8…

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Study inhibition activity of Myrtus communes leave aqueous extract against oral pathogens

الباحث الأول: D. wafa S . AL-waznee

الباحثين الآخرين: .D.mayada F. Darweesh , D . Najah

المجلة: جامعة كربلاء / المؤتمر العلمي الأول لكلية العلوم

تاريخ النشر: None

مختصر البحث:

This study Including explain the inhibition activity of Myrtus communes leave aqueous extract against oral pathogens that isolated from 56 swabs were taken from patient undergo gingivitis and dental caries. The gram positive bacteria were responsi…

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Test of efficacy of capsular polysaccharide antigen of Klebsiella pneumonia in stimulation of immune response in albino mice

الباحث الأول: Mayyada F. Darwesh*

المجلة: QMJ

تاريخ النشر: None

مختصر البحث:

The study was designed to assess in vivo the effects of capsular polysaccharide (CPS) antigen of K. pneumonia on some immunological parameters in albino mice. CPS antigen from K.pneumonia was prepared, then series concetration of wild type of ba…

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Immunological aspects on Asthmatic Patients تقييم بعض الجوانب المناعية لمرضى الربو

الباحث الأول: D.mayyada F.Darweesh

الباحثين الآخرين: مجلة جامعة الكوفة

المجلة: مجلة جامعة الكوفة Magazine of Al-Kufa University for Biolog

تاريخ النشر: None

مختصر البحث:

the study was designe to assess some immunological aspects of asthma patients by detected the total and differential count of leucocyte , immunoglobulin titer and specific activity of adenosine deaminase ADA . Therty sex samples of patients were col… the study was designe to assess some immunological aspects of asthma patients by detected the total and differential count of leucocyte , immunoglobulin titer and specific activity of adenosine deaminase ADA . Therty sex samples of patients were collected and 24 sample of healthy as control group . The result was revealed that the WBCs count increased in patients group compared with control group , and the neutrophils and basophile were increased significantly while, lymphocyte and monocyte was decreased. The concentration of IgE increased significantly but IgG was decreasedThe specific activity of ADA was highly significant (P≤ 0.01) increased compared with control group. الخلاصة :- تناولت الدراسة الحالية تقييم بعض الجوانب المناعية لدى المرضى المصابين بالربو مثل قياس معايير الدم وتشمل العدد الكلي والتفريقي لخلايا الدم البيض وقياس مستوى الاميونوكلوبيولينات المناعيةا(Ig) وقياس فعالية انزيم الادينوسين دي امينيز(ADA) بأعتباره انزيم مناعي ودراسة امكانية استخدامة كعلاج مناعي بدلا من العقاقير المستخدمة في علاج مرضى الربو لتجنب التأثيرات الجانية لبعض الادوية المستخدمة في العلاج ، وتضمنت اختيارا عشوائيا" لـ 36 مريض من مستشفى الصدر التعليمي في محافظة النجف بالاضافة الى 24 شخصا" سويا" بوصفهم مجموعة سيطرة . اظهرت نتائج الدراسة حدوث زيادة في العدد الكلي لخلايا الدم البيض لدى مجموعة المرضى وكانت الزيادة عالية المعنوية عند المقارنة مع مجموعة السيطرة ، كما بينت النتائج حدوث زيادة عالية المعنوية في عدد neutrophill and basophill cells وبنفس الوقت انخفاض في عدد ,Lymphocytes and monocytes cells.دلت النتائج انخفاض في مستوى مقابل زيادة في مستوى الاميونوكلوبيولين E ، ، وبالوقت نفسه بينت النتائج حدوث زيادة في الفعالية النوعية لانزيم ADA لدى مجموعة المرضى بالمقارنة مع مجموعة السيطرة مما يؤكد دوره في تحويل مادة الادينوسين السامة والتي لها دور اساس في تطور مرض الربو الى ساينوسين وهو مركب غير سام

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Profiles of miRNA expression and IFN- serum level as biomarker for the development of Multiple Sclerosis

الباحث الأول: Baydaa M. Abaas

الباحثين الآخرين: Mayyada F. Darweesh

المجلة: The Egyptian Journal of Immunology

تاريخ النشر: 1 يوليو، 2024

مختصر البحث:

Multiple sclerosis (MS) is associated with a wide spectrum of sensory, motor, and psychological disorders. Cytokines level and microRNA (miRNA) expression have roles in the disease's progression and the start of a damaging immune response in the cen…

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Immunopathological role of MiR-326, viral infection and IL-17 concentration in multiple sclerosis patients

الباحث الأول: Baydaa M. Abaas

الباحثين الآخرين: Mayyada F. Darweesh

المجلة: Fam Med Prim Care Rev

تاريخ النشر: 5 يونيو، 2024

مختصر البحث:

altered immune response with demyelination and loss of nerve axons. Objectives. This study aimed to focus on the role of MiR-326 in relation to CMV, HSV and IL-17 concentration in MS. Material and methods. A case-control study was carried out fo… altered immune response with demyelination and loss of nerve axons. Objectives. This study aimed to focus on the role of MiR-326 in relation to CMV, HSV and IL-17 concentration in MS. Material and methods. A case-control study was carried out for 100 participants classified as 80 multiple sclerosis patients (case group), which included 60 female and 20 male at different ages, at the Al-Husseini Teaching Hospital in the holy city of Karbala from November 2022 till end of April 2023, along with 20 apparently healthy subjects (control group). Approx. 4 ml of blood was taken from all participants, which was divided into 2 ml of blood to separate serum for detected IL-17 level by enzyme linked immunosorbent as say (ELISA) and examined CMV and HSV-1 antibodies, and by reverse transcription polymerase chain reaction (RT-PCR) technique 2 ml of blood used to investigate whether expression level of micR326 could be used as predictor for multiple sclerosis and its correlation with IL-17. Statistical analysis was completed with the use of statical software (IBM SPSS statistics 26). The chi-square test was used to determining the statistical difference of qualitative results among the different groups. The results of IL-17 level and miR-326 are given as arithmetic mean ± S.D. One-way ANOVA was used to analyze the comparison between patients and the control group. A p-value < 0.05 was considered statically significant. Results. The study revealed that females with multiple sclerosis more than males with a female: male ratio (3:1), the age of patients range from 18–63 and the 20–40 years were highest age group with 44 (55%). According to type of disease 22.5% of patients were SPMS and 77.5% were RRMS type, 20 (25%) of patients infected with HSV-1 14 (7.5%) of patients infected with CMV, while 46 (57%) of patients infected with both CMV & HSV-1. IL-17 level increased in the patients with multiple sclerosis as compared to healthy and have higher IL-17 serum level in SPMS than RRMS. Increase miRNA-326 mean fold change expression in patients (3.1 ± 1.65) in compare to healthy subjects (1.0 ± 0.23). Conclusions. This result bolsters the theory that there is a connection between viral infection multiple sclerosis, serum level concentra tion of interleukin-17 play key role in progressive disease, microRNA-326 may take part in the development of MS and microRNA-326 expression can be useful biomarker for prediction of multiple sclerosis.

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Investigate role of miRNA146a and IL-17 level in progressive rheumatoid arthritis disease

الباحث الأول: Wijdan A. Faihan

الباحثين الآخرين: Mayyada F. Darweesh

المجلة: The Egyptian Journal of Immunology

تاريخ النشر: 25 يوليو، 2024

مختصر البحث:

Rheumatoid Arthritis (RA) is a chronic, progressive autoimmune disease, involves an intimate relationship between immune cells and cytokines and results in decreased lifespans and higher mortality rates. The goal of the current study was to investig…

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Prognostic Value of miRNA-155 and Tumor Necrosis Factor-Alpha in Rheumatoid Arthritis Disease

الباحث الأول: Ali J. Almousawy

الباحثين الآخرين: Mayyada F. Darweesh

المجلة: Egyptian Journal of Medical Microbiology

تاريخ النشر: 9 يناير، 2025

مختصر البحث:

Background Rheumatoid arthritis (RA) is one of common autoimmune disorder globally affected 1% of population characterized by irregular cytokine production. microRNAs can influence the cytokine production that is associated with autoimmunity. … Background Rheumatoid arthritis (RA) is one of common autoimmune disorder globally affected 1% of population characterized by irregular cytokine production. microRNAs can influence the cytokine production that is associated with autoimmunity. Biomarkers found in local and systemic inflammation may give the solution for accurately diagnosing and treating arthritis. Objective: to evaluate TNF-α levels in synovial fluid and serum with serum miRNA-155 expression as prognostic markers in RA individuals. Methodology: 80 RA patients were attended to Al-Saddar Medical City Rheumatology Unit in the Najaf Governorate and 40 healthy as control group were selected for case control study. The mean patients' age was 50.6±16.5 and 42.5±9.24 years for healthy group. These patients were diagnosed by specialized physicians and given a diagnosis of rheumatoid arthritis then classified into mild (stage1 and 2) and aggressive stages (stage 3 and 4). Three ml of venous blood were taken from all participant from which 2 ml were placed in gel tube for serum separation to measuring RF and ACCP as confirmative diagnosis, the other 1ml was placed in Eppendorf tube with Triazole and immediately stored at -20 C◦ for miRNA -155 evaluation by RT-PCR, Knee synovial fluid was separated from Knee effusion of both RA patients and patients suffering from knee trauma only as control group. TNF-α was measured in serum and S.Fluid by ELISA assay. Results: that RA patients between the ages 45 - 67 years had a greater incidence and severity of the illness. TNF-α levels were substantially greater in patients with Aggressive-stage disease than in patients with mild-stage. According to ROC analysis appropriate cut-off value of miRNA-155 was 1.66, which had 0.944 % sensitivity, 0.889% specificity, AUC= 0.972, at P=0.000***. while the appropriate cut-off value of TNF-α appeared with high validity as prognostic factors in both serum and S. Fluid ( 87 % sensitivity, 97% specificity, AUC 0.992 in serum and 83.3% sensitivity, 86.7% specificity, AUC 0.915 in synovial Fluid. Conclusion: By using ROC analysis, it was possible to achieve good diagnosis accuracy for TNF-α levels in both serum and synovial fluid, as well as more sensitive and accurate discrimination of RA disease. TGF-β1 and miRNA-155 blood levels may help patients to avoid discomfort and stress instead of employing synovial fluid separation as a diagnosis for excellent prognosis in RA illness.

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ميادة فرحان درويش جلالده

ميادة فرحان درويش جلالده

الملف الشخصي

الاهتمامات البحثية

البحوث المنشورة

Bacterial Infection, IL-17 and Some Biomarkers with Related to Covid-19 Patients’ Outcome

The Immune-modulator Activity of Pseudomonas aeruginosa Extracted Protein: Azurin Charming Protein

Biofilm Formation Frequency and Drugs Resistance of Staphylococcus Aureus Isolated From Clinical Samples

The Role of IL-12 in the Aetiology of SLE and its Connection to HBV Infection in Iraqi Patients

The impact of bacterial prostatitis and IL-17 in male infertility

The Impact of IL-35, Bacterial Prostatitis in Development Male Infertility in Najaf Province Patients

Evaluate microbial profile and IL-17 level in transmission transfusion infection patients

Evaluate IL-10 level and gene polymorphism in β-Thalassemia patients with related to microbial infection and disease outcome

Evaluate IL-10 level and gene polymorphism in β-Thalassemia patients with related to microbial infection and disease outcome

Antibacterial effect of Cappariloside (C22H28N2O11) and Vitexlactam (C22H35NO4) alkaloids compounds isolation from Capparis spinosa roots & Vitex agnus-castus fruits.

Assesment of capslar antigen extracred from Proteus volgaris as immune stimulater and antibacterial activity

The role of antioxidants protein in regulated thalassemia patients state in relation with humeral immune system

The antibacterial & antibiofilm activity of Punica granatum peel aqueous extract against some oral pathogen

The Role of Interferon- Gene Polymorphism in Spontaneous Abortion

Study inhibition activity of Myrtus communes leave aqueous extract against oral pathogens

Test of efficacy of capsular polysaccharide antigen of Klebsiella pneumonia in stimulation of immune response in albino mice

Immunological aspects on Asthmatic Patients تقييم بعض الجوانب المناعية لمرضى الربو

Profiles of miRNA expression and IFN- serum level as biomarker for the development of Multiple Sclerosis

Immunopathological role of MiR-326, viral infection and IL-17 concentration in multiple sclerosis patients

Investigate role of miRNA146a and IL-17 level in progressive rheumatoid arthritis disease

Prognostic Value of miRNA-155 and Tumor Necrosis Factor-Alpha in Rheumatoid Arthritis Disease

المحاضرات

Immunology

lecture 1- immune system مناعه

lecture 2- immune system مناعه

Lecture -3- immunology