DETECTION OF PSEUDOMONAS AERUGINOSA PATHOGENICITY MARKERS IN CLINICAL SPECIMENS
الباحث الأول:
Sarah Hasen K. AL-Huchaimi
الباحثين الآخرين:
Mahdi H. Al-Ammar,Angham Jassim, Mahdi AL-Hadad
المجلة:
Biochem. Cell. Arch
تاريخ النشر:
11 سبتمبر، 2017
مختصر البحث:
ABSTRACT : Pseudomonas aeruginosa is an opportunistic pathogen particularly affects those with impaired host or mucosal
immunity and has a broad range causes impaired host or mucosal immunity and has a broad range of presentations, including
respi…
ABSTRACT : Pseudomonas aeruginosa is an opportunistic pathogen particularly affects those with impaired host or mucosal
immunity and has a broad range causes impaired host or mucosal immunity and has a broad range of presentations, including
respiratory infections in cystic fibrosis and mechanically ventilated patients, blood stream infections in premature neonates
and wounds in burns injuries in the healthcare setting. This study aimed to determine the P.aeruginosa that causes skin
infection by detecting of pathogenisity markers. Bacterial isolated and identificated phenotypically and genotypically, as well
as detects the pathogenicity markers encoding genes producing from P.aeruginosa by PCR techniques. About one hundred
twenty clinical specimens were collected from different clinical infections P.aeruginosaidentification according to colonial
morphology, microscopic examination and biochemical tests. Results show that P. aeruginosa had ability to produce capsule,
biofilm, adhesion ,protease, bacteriocin ,haemolysin, b-lactamase and gelatinase. Regard to molecular study the outcome
showed that 24 (100%) of P. aeruginosa isolates carrying lasB gene, 24(100%) carrying aprA, 23(95%) carrying plcH while
13(54%) carrying algD. Present results concluded that P. aeruginosa able to produce sever pathogenicity markers which
responsible for pathogenicity in any patients infected.
