مختصر البحث:
Abstract
Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the
world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral
t…
Abstract
Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the
world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral
tissues. G2548A polymorphism in the LEP gene promoter has been associated with insulin resistance, leptin, and type 2
diabetes mellitus across different population, but has not been inclusively reported within the Malaysian population.
Objective: Thus, our study aimed to investigate the impact of G2548A polymorphism on serum leptin levels and insulin
resistance among Malaysian T2DM patients.
Methods: This case-control study involved 150 T2DM patients and 150 non-diabetic volunteers from ethnic Malays,
Chinese and Indians. Genotyping of G2548A polymorphism was carried out using PCR-RFLP. Serum leptin and insulin
levels were determined via ELISA. ANOVA and Chi-square tests were used to determine the distribution of genotypes
and allelic frequencies based on serum leptin and insulin levels.
Results: Frequency of AA genotype and A allele of G2548A variant were significantly (P < 0.05) higher in T2DM
patients of Malay and Indian ethnicities (4%, 35%, and 36%, 57%, respectively) as compared to the control groups (0%,
22%, and 18%, 35%, respectively). Fasting serum leptin levels were significantly (P < 0.001) higher in T2DM patients
compared to non-diabetic subjects (166.78 pg/ml, 101.94 pg/ml, respectively). Additionally, elevated serum leptin, insulin
levels, and BMI in diabetic patients were found to be associated with the AA genotype of this variant, compared to GG,
and GA genotypes (P < 0.05).
Conclusion: Our findings suggest a significant association between G2548A polymorphism among Malaysian T2DM
subjects, particularly among Malay and Indian ethnic groups. Moreover, the A allele frequency of the G2548A variant
significantly increased the risk of T2DM and is significantly associated with increased serum leptin, insulin levels, and
elevated BMI.
Keywords: Leptin, insulin, G2548A polymorphism, type 2 diabetes mellitus, Malaysian's population
