study of fungal species isolated from thalassemia patients in Al-najaf province in iraq
الباحث الأول:
Ali Yas Khudhair Al-Amery
الباحثين الآخرين:
Jaffar Muhammad Baqir Muhammad Rashad
المجلة:
Chinese journal of industrial hygiene and occupational diseases
تاريخ النشر:
None
مختصر البحث:
Abstract Objective: This study was conduct to investigate phenotypic identification of fungal species isolated from Thalassemia patients in Al-Najaf province and evaluation inhibition activity for some antifungal agents (Nystatin and Amphotericin B)…
Abstract Objective: This study was conduct to investigate phenotypic identification of fungal species isolated from Thalassemia patients in Al-Najaf province and evaluation inhibition activity for some antifungal agents (Nystatin and Amphotericin B). Methods: During the period from October to November 2021, 50 samples were collected from AL-Zahraa Teaching Hospitalin AL Najaf province, which included swabs from the oral; only 14 samples gave positive growth. Cultivation of all samples in the Microbiology, Faculty of Medical techniques/Islamic University Najaf on Sabouraud dextrose agar and then on CHROMagar medium for the purpose of diagnosing the species of Candida depending on the color of the colonies Results: The results were diagnosed on CHROMagar medium as follows: Calbicans 10(71.4%), followed by C. parapsilosis 3(21.4%). C. tropicalis1(7.1%). In this study, the ability of Calbicans was tested to produce some virulence factors such as germ tube, biofilms and production of enzymes phospholipases when culturing Candida spp. on different media. The results of the culture show that C.albicans produces all virulence factor included germ tube, biofilms and phospholipases enzyme. The result of this study for the antifungal activity for (Nystatin and Amphotericin B), explaining the effects against Candida spp. by using disk diffusion method. These results show Amphotericin B is more effect than Nystatin against Candida spp. Conclusion: We can consider they east species as the most common fungal etiological agent of life threatening invasive infections in Thalassemia patients who are severely immunocompromised and treatment which requires extended stay in intensive care units
