Effect of polymorphism on CTLA-4 gene on Graves' Disease
الباحث الأول:
Eman Th. Al-Fatlawy1
الباحثين الآخرين:
Israa K. Al-Yasiri2
, Ibtihal Al-Shamarti3
المجلة:
©Annals of Tropical Medicine & Public Health S276
تاريخ النشر:
None
مختصر البحث:
Graves is one of autoimmune Thyroid Diseases. Moreover, more than 20 genes polymorphisms are
associated with Graves’s diseases. Although, the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) genes play an
important role in immunity system func…
Graves is one of autoimmune Thyroid Diseases. Moreover, more than 20 genes polymorphisms are
associated with Graves’s diseases. Although, the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) genes play an
important role in immunity system function, strategies to enhance CTL4 polymorphism gene may be impact on increase
Graves' disease. Recent research has revealed that the 49A/G polymorphism in exon 1 has a detrimental EFFECT ON this
disease, results in a threonine-to-alanine conversion. Objectives: The study was carried out to investigate and affect
allele polymorphism CTL4 gene in Graves' disease in AL-Najaf province. Methods: In our study, 73(48 females and 25
males) individuals who detected Autoimmune hyperthyroidism and twenty healthy as a control groups. All Graves
patients were recruited from the hormonal unite at Al-Sadder Medical City, Najaf/Iraq. The control group participating
in the study did not have any history of immune diseases and chronic diseases. The study was approved by the ethical
committees of Al-Sadder Medical City. Genomic DNA was extracted then polymerase chain reaction fragment length
polymorphism (PCR-RFLP) technique is used, follow by Sequence data of polymorphism. Results: Results of this study
found that all samples were digested with restriction enzyme Fnu4HI in two fragments (99 and 63) bp. These results
indicate that 49 A/G polymorphism GLT4 gene was detected in Graves patients and control. Conclusion: Contrary to
expectations, this study did not find a significant difference between Graves patients and healthy individuals in the Najaf
city population.
