Ukufa

الملف الشخصي

خريج كلية العلوم قسم الكيمياء/ جامعة الكوفة عام 1998. حاصل على شهادة الماجستير في الكيمياء عام 2006 من كلية التربية ابن الهيثم/ جامعة بغداد. احد منتسبي فرع الكيمياء الحياتية/ كلية الطب منذ عام 2000. حاصل على شهادة الدكتوراه في الكيمياء الحياتية من جامعة شفيلد/ المملكة المتحدة.

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الاهتمامات البحثية

بروتين و جين بولمورفزم وعلاقتها ببعض الامراض المزمنة

البحوث المنشورة

THE RELEVANCE OF SECRETED FRIZZLED-RELATED PROTEIN 4 AND IRISIN AS PREDICTIVE MARKERS FOR GESTATIONAL DIABETES MELLITUS PATIENTS

الباحث الأول: Dunia M. Mohammed Ali

الباحثين الآخرين: Ibrahem Rahem Jassim

المجلة: Biochem. Cell. Arch

تاريخ النشر: None

مختصر البحث:

ABSTRACT : Gestational diabetes mellitus (GDM) was define the metabolic disorder in pregnancy majoring to acute plus chronic complications in mothers as well asnew-borns. Previous research has appeared to demonstrate an association between both secr… ABSTRACT : Gestational diabetes mellitus (GDM) was define the metabolic disorder in pregnancy majoring to acute plus chronic complications in mothers as well asnew-borns. Previous research has appeared to demonstrate an association between both secreted frizzled related protein 4 (SFRP4) plusirisin values on the one hand and the occurrence of Type2 Diabetes, although there is no comparable evidence in respect to GDM. Therefore, the aim of the study to conclude, if there is an connection between SFRP4 and GDM and likewise for irisin and if so to explore the connection between these markers and glycaemic parameters. The present case control study included a group of 60 women of GDM and a group of 30 healthy pregnant women (control group). Both groups were subjected to clinical examination encompassing fasting serum glucose (FSG), oral glucose tolerance test (OGTT), HbA1C plus lipid profile levels determined by spectrophotometric methods. Fasting serum insulin, SFRP4 and irisin concentrations were measured in sera by the ELISA technique. Body mass index (BMI) plus HOMAIR were also calculated. The data was analyzed and articulated in terms of (Mean±SD). Person correlation was undertaken in order to study the serum correlation of SFRP and irisin to glycaemic control and lipid profile in the studied groups. The present study show that the mean age value was identical in thetwo groups. Median BMI, fasting serum glucose, OGTT at 1 hour, OGTT at 2 hours, HbA1C and serum insulin values were elevatedof GDM patients when compared to healthy pregnant women, The median SFRP4 values were increased in the GDM patients group; while the median irisin values were increased in the control group. Serum SFRP4 values were elevated and irisin values reduced in GDM patients.Theseresults recommended that serum SFRP4 and irisin values could be introduced as predictor markers for GDM. Additional investigations are wanted to discover the “biological effects” of SFRP4 and irisin going on pregnant women.

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Investigation into the level of Cellular Prion Protein (PrPc) in Glioblastoma Multiforme (GBM) Cells Treated with Alkylating Agents and Other Small Molecules

الباحث الأول: Ibrahem Al-Aadily

المجلة: University of Sheffield

تاريخ النشر: None

مختصر البحث:

Abstract Glioblastoma multiforme (GBM) is a malignant tumour of astrocytes in the brain which accounts for approximately one third of all tumours in the nervous system. Despite the availability of chemotherapy, the median survival rate for all patie… Abstract Glioblastoma multiforme (GBM) is a malignant tumour of astrocytes in the brain which accounts for approximately one third of all tumours in the nervous system. Despite the availability of chemotherapy, the median survival rate for all patients is between 15 to 24 months. Temozolomide (TMZ) and carmustine (BCNU) are the most common chemotherapeutic agents used in combination with radiotherapy or surgery to treat GBM. The overexpression of PrPc in GBM has been reported and its involvement in the resistance to alkylating agents (TMZ and BCNU) was speculated. The exact role of PrPc in GBM, i.e. how it is affected by chemotherapeutic agents and small molecules, needs to be further investigated. In this work, the effect of TMZ and BCNU on PrPc was evaluated using U87 glioblastoma cell line. The results showed that 24 h treatment with TMZ or BCNU followed by 48 h resting gave the highest level of G2/M cells (about 84%) when compared with untreated cells. The upregulations of p53 and p21 are believed to be the key cause for this increment. PRNP gene is also upregulated in TMZ or BCNU treated U87 cells for 24 h treatment followed by 48 h resting and this may contribute to the resistance these cells towards the treatments due to the cytoprotective effect of this protein despite less PrPc is detected at protein level. Some of these findings are in agreement while others are opposite to those previously reported. The resistance to chemotherapy is considered as one of the main limitations for the effective treatment of cancer, especially for GBM. PRNP gene in U87 cell line was silenced to determine cell proliferation and chemoresistance. A decrease of 80% in PrPc level was obtained after 48 h of treatment with transfection reagents. Additionally, the level of the PrPc knockdown was retained 5 days post-knockdown. Silencing of the PRNP gene in glioblastoma cell line U87 reduces cell proliferation in a time-dependent manner. In addition, positive cells with proliferation marker ki67 were significantly decreased in knockdown cells after 5 days. This implies that PrPc contributes to the malignance of the cancer cells. It was also found that there was no significant reduction in cell proliferation in response to TMZ or BCNU in knockdown cells and p21 is upregulated in TMZ or BCNU knockdown cells. Therefore, silencing PRNP gene may impact the function of p21 which contributes to the resistance to alkylating agents TMZ and BCNU. In an attempt to sensitise U87 cells to TMZ, the combination treatment of MG132 with TMZ did not improve the apoptosis rate and no accumulative effect was observed. High concentration of MG132 increased the level of PrPc in U87 cells after a short incubation time. The level of PrPc was also evaluated in U87 cells treated with the compounds (FAPi) which inhibit the Fancony Anaemia (FA) pathway by reduction of FANCD2 using high content imaging by ImageXpress Micro. These compounds did not affect the level of PrPc in these cells with or without combination with TMZ or BCNU showing that these FAPi are target specific. Several novel families of heteroaromatic compounds (30 compounds) previous shown to be cytotoxic to embryonic carcinoma cells (NT2) were screened on U87 cells and eight of them were found to be inhibit the proliferation of the cells. The compound 175 that belongs to the acridine family induced apoptosis for U87 cells. It also arrests U87 cells in G2/M and upregulate p53 and p21 proteins which are regulatory for cell cycle. That may open the door to examine more acridine compounds as anti-glioblastoma agents.

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Insight into adiponectin gene impact on coronary artery disease from a polymorphism case-control study in Iraqi individuals

الباحث الأول: Hussain, M.K

الباحثين الآخرين: Ibrahem Rahem J. Al-Aadily , Alhan Abdulmohsin A. Al-Rashid , Abdol Hussein A. Algenabi , Ahmed Naseer Kaftan , Muna Abdulridha Al-Barqaawi , Layth Ahmed A. Al-Fahham , Teba Jaber Merza , Iman Jabbar Kadhim , Mohammed Ali Abdulameer Khikani

المجلة: Gene Reports

تاريخ النشر: 1 سبتمبر، 2024

مختصر البحث:

Background Coronary artery disease (CAD) is a group of diseases caused by plaque formation in the wall of arteries supplying blood to the heart. Adiponectin (ADIPOQ) is an adipocytokine that controls carbohydrate and lipid metabolism, has anti-diab…

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Anticancer Effect of Sargassum oligocystom Hydroalcoholic Extract Against SW742, HT-29, WiDr, and CT-26 Colorectal Cancer Cell Lines and Expression of P53 and APC Genes

الباحث الأول: Ibrahem Rahem Jassim Al-Aadily

الباحثين الآخرين: Suzan Ibrahim Bajilan, Dhafer A. F. Al-Koofee & Ali H. Al-Marzoqi

المجلة: Journal of Gastrointestinal Cancer

تاريخ النشر: 9 يناير، 2022

مختصر البحث:

Colorectal cancer (CRC) is the third most common cancer in the world, with enhancing morbidity and mortality each year. Due to the drug resistance against CRC, the use of novel compounds besides chemotherapy is required. Natural seafood contains lar…

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ابراهيم رحيم جاسم العادلي

ابراهيم رحيم جاسم العادلي

الملف الشخصي

الاهتمامات البحثية

البحوث المنشورة

THE RELEVANCE OF SECRETED FRIZZLED-RELATED PROTEIN 4 AND IRISIN AS PREDICTIVE MARKERS FOR GESTATIONAL DIABETES MELLITUS PATIENTS

Investigation into the level of Cellular Prion Protein (PrPc) in Glioblastoma Multiforme (GBM) Cells Treated with Alkylating Agents and Other Small Molecules

Insight into adiponectin gene impact on coronary artery disease from a polymorphism case-control study in Iraqi individuals

Anticancer Effect of Sargassum oligocystom Hydroalcoholic Extract Against SW742, HT-29, WiDr, and CT-26 Colorectal Cancer Cell Lines and Expression of P53 and APC Genes

المحاضرات

محاضرة 1 السشن الاول / Introduction to Cells and Biomolecules وحدة الجزيئات والجين والمرض

محاضرة 2 السشن الثاني/ Oxygen transport proteins Lecture 4 وحدة الجزيئات والجين والمرض

Carbohydrate Metabolisn 2 وحدة الايض

Control of Metabolism وحدة الايض