Real Time PCR Detection, Sequencing, and Phylogenetic
Tree Analysis of Newcastle Diseases Virus Isolated from an
Outbreak in Layer Flocks in Baghdad Capital, Iraq
الباحث الأول:
Hussam Muhsen1, 4
الباحثين الآخرين:
Abdullah O. Alhatami3, Yahia Ismail khudhair, Furkan Alaraji2
المجلة:
Indian Journal of Public Health Research & Development, August 2019, Vol.10, No. 8
تاريخ النشر:
None
مختصر البحث:
The definition of the Newcastle disease (ND) under the World Organization for Animal Health strategy is
that chicken which infected with ND virulent (NDV) strains of avian paramyxovirus (APM). The current
work included the identification of pathog…
The definition of the Newcastle disease (ND) under the World Organization for Animal Health strategy is
that chicken which infected with ND virulent (NDV) strains of avian paramyxovirus (APM). The current
work included the identification of pathogenic properties (PPs) of serotype 1 belongs to AP that was
detected in samples collected from severe-ND-affected chickens. The detection was performed measuring
heamaglutination inhibition (HI), intracerebral pathogenicity index (ICPI), and mean death time (MDT)
for in vivo exploration and real time-quantitative polymerase chain reaction (RT-qPCR), restriction enzyme
analysis (REA), and DNA sequencing of the F2/F1 cleavage site for the in vitro investigation. This study is
the first genetic-based analysis of the ND virus in Iraq. Complete correlation between the in vivo and in vitro
results was recognized. The findings of the in vivo investigation showed that the strain was pathogenic and
at the same time the structural patterns of the restriction plus the sequence of the amino acids were typical
for virulent strain which resemble with 99% Iranian strain.
This study adds new information about the pathology and antigen distribution of this novel virus in Iraq and
additional work is needed to get a complete data base of viral diversity and procedure for vaccinating and
eliminating from this disease in our country.
