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In the current study, nontoxic selenium nanoparticles were produced extracellularly utilizing probiotic Bacillus clausii bacteria, and their antibacterial efficacy against multidrug-resistant bacteria was evaluated. Initially, the biosynthesis of Se…
In the current study, nontoxic selenium nanoparticles were produced extracellularly utilizing probiotic Bacillus clausii bacteria, and their antibacterial efficacy against multidrug-resistant bacteria was evaluated. Initially, the biosynthesis of SeNPs was indicated by the transformation of the reaction mixture from yellow to dark red. UV-Visible spectrophotometry was used to characterize biosynthetic nanoparticles, and the greatest absorbance peak of nanoparticles was recorded at wave lengths of (270nm). Nanoparticle size, shape, and distribution were all detected using a scanning electron microscope (SEM). The nanoparticles had a spherical, homogeneous shape, and their sizes ranged from (40-150nm). Energy Dispersive-x-ray Spectroscopy (EDS) investigation of the presence of elemental selenium.. Biosynthesized AgNPs exhibited antibacterial activity against the majority of multidrug-resistant bacteria (MDR), such as Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumonia, Acinetobacter baumannii, Proteus mirabilis, pseudomonas aeruginosa, and Shigella sonii, when compared to the results of an antibiotic disc of Ceftriaxone, Azithromycin, Cefepime, Amikacin, Meropenem, Oxacillin, Gentamicin and Ciprofloxacin against such clinical bacteria. According to the findings, probiotics and SeNPs will be used to enhance or replacement of antibiotic
