Molecular characteristics of some Botrytis isolates
and antagonistic activity of Bacillus subtilis and its
bacteriocin on tomato fruit
الباحث الأول:
Firas A. Ahmed
الباحثين الآخرين:
Ali M. Salman & Basil H. Kandouh
المجلة:
Taylor and Francis
تاريخ النشر:
2 نوفمبر، 2023
مختصر البحث:
Gray mold, caused by Botrytis cinerea, is one of the most
devastating agricultural diseases during the pre and posthar
vest stages. this study aims to investigate the application of
Bacillus subtilis as a bioagent against B. cinerea and reduci…
Gray mold, caused by Botrytis cinerea, is one of the most
devastating agricultural diseases during the pre and posthar
vest stages. this study aims to investigate the application of
Bacillus subtilis as a bioagent against B. cinerea and reducing
pre and postharvest disease as a replacement method to
chemical pesticides. Five Botrytis isolates were molecularly
confirmed and the virulent isolate Bc2 was registered for the
first time in the National center of Biotechnology information
(NcBi) with accession number OP257237. the mycelial
growth of B. cinerea was inhibited by up to 84% when
treated with the bio agent bacterium using the double
drawing method in Petri plates. Pre-harvest application of B.
subtilis at a concentration of 108/ml cFU reduced posthar
vest -gray mold of tomato fruit during ripening stages in the
greenhouse. Preharvest application of bioagent bacterium
B. subtilis induced the levels of B-1, 3-glucanase and
Polyphenol oxidase, indicating enhanced plant resistance to
gray mold. Disease severity was decreased compared with
control and extended the shelf life at 25 °c.the field applica
tion of the bio agent bacterium at the maturity of ripening
in preharvest had a significant effect on increasing the level
of polyphenol oxidase PPO and B-1, 3-glucanase, which are
related to the enhancement of plant defenses and resis
tance. additionally, it led to an increase in storage life and a
reduction in post-harvest losses. the results demonstrated
that preharvest application of bio agent bacterium Bacillus
subtilis improved the quality of postharvest tomato fruit and
significantly reduced gray mold incidence, all without the
use of chemical pesticides.
