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The study was conducted on 350 aborted women and thirty healthy women, whom have visited Central public Health Laboratory in Al-Najaf Governorate during the period from November 2014 to May, 2015. This study was designed to investigate of some tes…
The study was conducted on 350 aborted women and thirty healthy women, whom have visited Central public Health Laboratory in Al-Najaf Governorate during the period from November 2014 to May, 2015. This study was designed to investigate of some tests which determine the infection with T.gondii in clinical suspected women in an- Najaf governorate by using two methods, detected specific IgG and IgM by VIDAS technique and PCR technique to indicate Toxoplasma gondii. Furthermore, it is designed to determine the effects of T.gondii infection on some parameters such as blood cells, cytokine and immunoglobulins besides, the results indicate that PCR were the best methods that can be used in diagnosis and to determine the prevalence of T.gondii. Yet the blood parameters gave a clear understanding to physicians to cure patients. Whereas the numbers, percentage, sensitivity and specificity of infected women vary with different tests were 67 (19.14%) (93.05) (98.31), while PCR gave the highest numbers, percentage, sensitivity and specificity were 71 (20.28%) (98.61) (99, 64) respectively for each test. DNA of T.gondii parasite was determined by PCR was using three specific primers (B1, 18S and P30) whereas the number, percentage and sensitivity of infected women of these primers were 71 (20.28%) (98, 61%), 66 (18.85%) (91.66%), and 62 (17.71%) (87.32%) respectively. By comparing these primers to evaluate the efficiency in diagnostic of T.gondii. The results indicated that B1, 18S are the highest primers and P30 is the lowest primers in diagnostic efficiency. The results showed a significant elevation (P<0.05) in serum concentration of pro inflammatory cytokines interleukin-5 (IL-5), interleukin -27 (IL-27) and immunoglobulin M, while no significant (P>0.05) in level of immunoglobulin G in T.gondii infection patients compared to control group. However, the total Leukocyte count showed significant increase (P< 0.05) in infected women compared to the control group due to elevation percentage of neutrophil, monocyte, eosinophil, and lymphocytes while the number of Basophil, remain normal. There were positive correlation between proinflammatory cytokines interleukin-5 (IL-5), interleukin-27 (IL-27) and neutrophil, lymphocytes and IgM levels. The current study has also revealed positive correlation between lymphocytes and immunoglobulin M. The results have also exhibited a negative correlation between Il-5, IL-27 and IgG. Also, revealed a negative correlation between neutrophil, lymphocyte and IgG. The current study has concluded that the infection with T.gondii effect on blood cells, immunity of the body and the PCR test are the best methods and B1is the best primer used in the diagnosis of T.gondii parasite.
