Effect of Different Concentrations of Egg Yolk and Virgin Coconut Oil in Tris-based Extenders on Chilled and Frozen-thawed Bull Semen
الباحث الأول:
Tarig, A. A.
الباحثين الآخرين:
Wahid, H., Rosnina, Y., Yimer, N., Goh, Y. M., Baiee, F. H., Khumran, A. M., Salman, H., and Ebrahimi
المجلة:
Animal Reproduction Science
تاريخ النشر:
None
مختصر البحث:
The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen–thawed bull semen. A total of 24 ejaculates from four bulls were c…
The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen–thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4 °C until evaluation after 24, 72, and 144 h; the second fraction was processed by chilling for 3 h at 4 °C to equilibrate, then packaged in 0.25 mL straws and frozen and stored in liquid nitrogen at -196 °C until evaluation after 7 and 14 days. Both chilled and frozen semen samples were then thawed at 37 °C and assessed for general motility using computer-assisted semen analysis (CASA), viability, acrosome integrity, and morphology (eosin-nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS). The results indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater sperm quality (P < 0.05) as compared with the control. The treatment with 20% egg yolk had the greatest sperm quality (P < 0.05) among the treated groups for both chilled and frozen-thawed semen. In conclusion, the use of 8% VCO combined with 20% egg yolk in a Tris-based extender enhanced the values for chilled and frozen-thawed quality variables of bull sperm.
