Occurrence of RB
-
tumor suppressor gene overexpression in
woman suffering with cervical carcinomain mid Euphrates
الباحث الأول:
Elhamjwad
kadhem
1
College of education of Girl
,
University of Kufa
,
Kufa
,
Iraq
الباحثين الآخرين:
Azhar
Emran
Althahab
College of science
,
Department of biology
,
University of Babylon
,
Hilla
,
Iraq2
المجلة:
International Journal of
Pharm
Tech
Research
تاريخ النشر:
None
مختصر البحث:
Abstract
:
The retrospective research was designed as immunohistochemical
study to
demonstrate the expression state of tumor suppressor genes (RB) in (56) formalin
-
fixed,
paraffin embedded cervical tissues were …
Abstract
:
The retrospective research was designed as immunohistochemical
study to
demonstrate the expression state of tumor suppressor genes (RB) in (56) formalin
-
fixed,
paraffin embedded cervical tissues were included: 27 with malignant cervical carcinoma (CC)
and 16 with benign cervical tumors, 13 apparently healthy cervica
l tissues were used as a
control group.
The age
of these individuals (patients and control groups) ranged between 23
and 73 years. They were collected from the pathological archives of teaching laboratories at
many different private histopathological lab
oratories in Babylon, AL
-
Najaf, Kerbela, AL
-
Qadisiya, during the period from April 2015 to January 2016. After sectioning of these cervical
biopsies and staining by hematoxyline and eosin, a final definitive histopathological diagnosis
was done by a consul
tant histopathologist. The obtained results are summarized as follows:
The
mean age of the patients with cervical carcinoma (54
+
11.22years) was higher than the mean
age of the benign group (38.8
+
9.85 years) and the mean age of those females in the group
of
healthy control (35.5
+
8.64years). There are significant differences ( p< 0.05) between different
groups according to age. The well differentiated grade of CC constituted 52%, whereas those
with moderately and poorly differentiated grades CC constituted
37%and 11% respectively.
Over expression of RB
-
TSG was detected by IHC in 59.3 % of cervical cancer cases and in
43.8% of benign cervical tumor group, while none of control group showed RB
-
over
expression. A high percentage 43.6% showed of cervical canc
er have a moderate score (score
II). Flowed by 37.5% and 18.8% were found to have low score (score I) and strong score (score
III), respectively. While, in benign cervical tumor group, the highest
percentage 57.1% were
found to have moderate score (score I
I) and 28.6%, 14.3% have strong score (score III) and low
score (score I) respectively
.
The highest percentage of RB protein expression it was found within poorly differentiated that
have 100% (3 out of 3 cases). Followed by 60% (6 out of 10cases), 57.1 %(
8 out of 14 cases)
within moderately, well differentiated respectively
.
Keywords:
RB
-
tumor, gene overexpression, cervical carcinomain, mid Euphrates.
