Identification And Diagnosis Of Cutaneous Leishmaninsis By
Polymerase Chain Reaction Assay (Pcr) In Najaf Alashraf
Province
الباحث الأول:
Ban Shakir Alshukur
الباحثين الآخرين:
Haitham Mohammed Alawadi
المجلة:
Kufa Journal For Nursing Sciences
تاريخ النشر:
25 أغسطس، 2013
مختصر البحث:
Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR)
technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample
collected from lesion exudates patie…
Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR)
technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample
collected from lesion exudates patients.
Methodology: The (115) sample were examined by smear slid preparation culture on RPMI 1640 and NNN
then DNA isolation. The DNA of the promastigote were amplified by PCR including primers selected on
repetitive KDNA for identification of leishmania species.The data was analyzed by using frequency and
percentage.
Results: The PCR result showed that two species of leishmania (L. major with 620 bp and L. tropica with 800
bp) exist.
Conclusion: It is concluded from the present study that the PCR technique has high specificity and sensitivity
during the differentiation between Cutaneous Leishmanial species in comparing with conventional methods.
Recommendation: The PCR technique can be use it to diagnosis and differentiation between different type of
Cutaneous Leishmaniasis.
