Graphene oxide-induced, reactive oxygen species-mediated mitochondrial dysfunctions and apoptosis: high-dose toxicity in normal cells
الباحث الأول:
Ameer Ali Imarah
الباحثين الآخرين:
Jabir, Majid S
b
Send mail to Jabir M.S.;
Abood, Ali H
a;
Sulaiman, Ghassan M
b;
Albukhaty, Salim
c;
Mohammed, Hamdoon A
d, e;
Khan, Riaz A
d
Send mail to Khan R.A.;
Al-Kuraishy, Hayder M
f;
Al-Gareeb, Ali I
f;
Al-Azzawi, Waleed K
g;
A Najm, Mazin A
h;
Jawad, Sabrean F
i
المجلة:
Elsevier
تاريخ النشر:
None
مختصر البحث:
im: The cytotoxic effects of graphene oxide nanoparticles (GONPs) using MTT assays, observance of apoptotic markers, and oxidative stress were outlined. Materials & methods: Rat embryonic fibroblasts (REFs) and human epithelial breast cells (HBLs) w…
im: The cytotoxic effects of graphene oxide nanoparticles (GONPs) using MTT assays, observance of apoptotic markers, and oxidative stress were outlined. Materials & methods: Rat embryonic fibroblasts (REFs) and human epithelial breast cells (HBLs) were used at 250, 500 and 750 μg/ml concentrations. Results: Significant cytotoxic and apoptotic effects were observed. Analyses of CYP2E1 and malondialdehyde concentrations in REF and HBL-100 cell lines after exposing to GONPs confirmed the nanomaterials toxicity. However, the glutathione levels in REF and HBL-100 cell lines showed a substantial reduction compared with the control. The cytochrome CYP2E1, glutathione, malondialdehyde and caspase-3 alterations provided a plausible interlinked relationship. Conclusion: The study confirmed the GONPs cytotoxic effects on REF and HBL-100 cell lines. The outcome suggested caution in wide-spread applications of GONPs, which could have implications for occupational health also. © 2023 Future Medicine Ltd.
Author keywords
apoptosis; cytotoxicity; free radicals; GONPs; graphene oxide; high-dose vulnerability; nanomaterials; nanoparticles; nanotoxicity; oxidative damage; oxidative stress; ROS; toxicity
