مختصر البحث:
Abstract
The plant extracts and their chemical components have a long history as antifungal agents to control
the growth of isolations that released toxins and for this reason, they were used in the bio techniques
as a preservative due to incre…
Abstract
The plant extracts and their chemical components have a long history as antifungal agents to control
the growth of isolations that released toxins and for this reason, they were used in the bio techniques
as a preservative due to increased fungal resistance to the fungal antibiotics. The study aimed to
evaluate the efficiency of the alcoholic and aqueous extracts for Ajwa dates in inhibiting the production
of Ochratoxin A from Aspergillus Niger, and study the possible protective effect of these extracts on the
tissues of vital organs such as liver and kidneys against the toxicity that induced by Ochratoxin A. The
analysis results of the chromatography methods (HPLC, TLC) showed an influence of these two
extracts on the production of Ochratoxin A, and the full inhibitory impact for the production of this
type of fungal toxin was in the two concentrations ( 25, 20 mg / mL) by using the medium of Yeast
Extract Sucrose (YES). The present study did not find any significant differences (P˃0.05) between the
activity of aqueous and alcoholic extract in Ochratoxin A inhibition. The present study performed in
the animal house of Biology department/Faculty of Education for Girls, twenty laboratory male rats of
Sprague-Dawley strain were divided into four equal groups, the first group of animals (control) was
orally administrated with normal saline solution (0,9%),the second group was orally given alcoholic
extract of Ajwa dates at the concentration of (1) mg/kg, while the third group was orally treated with
Ochratoxin A at the concentration of (289) μg /kg ,with regard to the last group was orally received
alcoholic extract of Ajwa dates (1) mg/kg + Ochratoxin A (289) μg /kg respectively, the treatment of all
animals was once daily for five days a week. During this study the body weight as well as the relative
weights of liver and kidneys for rats were recorded, also the effectiveness of some hepatic enzymes
(Aspartate amino transferase AST, Alanine amino transferase ALT and Alkaline phosphatase ALP)
was estimated, in addition to the other biochemical parameters such as urea and creatinine levels, also
the study was comprised the preparation of histological sections for liver and kidneys. The statistical
analysis results of the current study were revealed ,Ochratoxin A caused a significant decrease
(P˂0.05) in the body weight at the group of rats that treated with it when compared with the other
experimental groups ,contrary to that, the Ochratoxin A showed a significant increase (P˂0.05) in the
relative weights of liver and kidneys ,effectiveness of hepatic enzymes AST,ALT , ALP, levels of urea
and creatinine at the male rats were submitted to this type of mycotoxin compared to the other groups
of study. In relation with the microscopic examination of liver and kidney sections for the laboratory
animals that treated with Ochratoxin A, several patho-histological changes were noted as compared
with the other groups. From the results of this study can be concluded that the alcoholic extract of
Ajwa dates was ameliorated the functional performance of both liver and kidneys through its
preservative properties to protect the tissues of those organs from the nephrotoxicity and
hepatotoxicity that induced by Ochratoxin A.
Keywords: Ochratoxin A, Ajwa dates, Path
