مختصر البحث:
Background: Pseudomonas aeruginosa considered of one of the most dangerous opportunistic bacteria worldwide, live in hospitals and other environments such as sewage water and soil and cause different infections include pneumonia, liver abscess and b…
Background: Pseudomonas aeruginosa considered of one of the most dangerous opportunistic bacteria worldwide, live in hospitals and other environments such as sewage water and soil and cause different infections include pneumonia, liver abscess and burns infections that may lead to death. Pseudomonas aeruginosa characterized by it’s highly resistance to antibiotics and it has several virulence genes make this pathogen is able to invade of human and animal. Therefore, the main aim of this study was to investigate of antibiotics susceptibility pattern and genotypic detection of virulence gens in P. aeruginosa isolates from clinical and environment sources. Materials and Methods: This is a case control study, a total of 120 P. aeruginosa isolates were collected from two sources; 60 isolates were collected from sputum of patients with pneumonia and 60 isolates were collected from sewage water. Antibiotics susceptibility testing was done according to Kirby-Bauer method for ten antibiotics and phenotypic test of extended spectrum β-lactamase (ESBL) was done according to synergistic effect between four antibiotics. Polymerase chain reaction technique was performed to detect the prevalence of six virulence-associated genes; Pap A, Fim H, feoB, iutA, hly and kpsMTII. Results: All 120 isolates (100%) were resistance to Amoxicillin. While, Imipenem was the best efficient antibiotics against clinical isolates (7 out of 60, 11.6%) and sewage water isolates (2 out of 60, 3.3%). Out of 60 clinical isolates, there were; 55 isolates (91.6%) were Multi-drug resistance (MDR), 9 isolates (15%) Extensive-drug resistance (XDR), 1isolates (1.3%) Pan-drug resistance (PDR) and 18 isolates (30%) were ESBL-producing isolates. While, out of 60 sewage water isolates, there were; 41 isolates (68.3%), 5 isolates (8.3%) and 1 isolate (1.3%) were MDR, XDR and PDR, respectively, and 11 isolates (18.4%) were ESBL-producing isolates .The Pap A, Fim H, feoB, iutA, hly and kpsMTII genes were prevalence in clinical isolates with percentages 91.6%, 100%, 80%, 95%, 20% and 100% respectively, while the same genes were prevalence in sewage water isolates with percentages, 51.6%, 68.3%, 41.6%, 35%, 3.3% and 5% , 41.6% respectively.
Conclusions: There was significant increase in antibiotics resistance between clinical and sewage water isolates of P. aeruginosa. While, there was high significant increase in the prevalence of virulence genes in clinical isolates as compared with sewage water isolates. This study suggested that there was positive correlation between the high prevalence of virulence-associated genes and increase of antibiotics resistance in P. aeruginosa isolates.
