Antibacterial Activity of Zinc Oxide
Nanoparticles-Poly-β hydroxybutyrate
Bionanocomposites on Staphylococcus spp
الباحث الأول:
Ali Shannan Alaredhey
The Islamic University of Najaf, College of Medical Technology, Department of
Medical Laboratory Technology, Iraq
الباحثين الآخرين:
Dhafer Rahman Al-janabi
The Islamic University of Najaf, College of Medical Technology, Department of
Medical Laboratory Technology, Iraq
Adil Ibadi Al-luhaiby
University of Kufa, faculty of science, department of laboratory investigation, Iraq
المجلة:
International Journal of Health Sciences
تاريخ النشر:
None
مختصر البحث:
In the laboratories of the College of Medical
Technologies/The Islamic University of Najaf, the biosynthetic zinc
nanoparticles were synthesized by Lactobacillus bacteria. The
structural and morphological properties of Zinc Oxide Nanoparticles
w…
In the laboratories of the College of Medical
Technologies/The Islamic University of Najaf, the biosynthetic zinc
nanoparticles were synthesized by Lactobacillus bacteria. The
structural and morphological properties of Zinc Oxide Nanoparticles
were determined by Atomic force microscope and X–ray diffraction
analysis, (hexagonal phase) shows that the synthesized nanoparticles
were crystalline and pure in nature. The peaks at 2θ = 38.1, 44.18,
64.45, 77.12 & 81.03 were respection lines of spherical Zinc Oxide
Nanoparticles respectively. Then, maximum diameter measured for
particles is 8.4278 nm which determined by applying the Scherrer
equation. After that, the samples of pathogenic bacteria
(Staphylococcus aureus, Staphylococcus Epidermidis) diagnostic by
Vitek2 compact system resistant to commercial antibiotics were
collected and the antibacterial activity of these nanoparticles was
tested. The results of inhibiting the growth of pathogenic bacteria were
good. Then the ZnONps were combined with a polymeric compound
(Poly-β hydroxybutyrate) to improve the properties of the
nanocomposite as an antimicrobial, as the combined compound
)ZnONPs/PHB) gave great inhibition results compared to the
nanocomposite alone(ZnONPs). Four different concentrations of ZnO
NPs /PHB (0.2, 0.1, 0.07 and 1.25 mg/ml) were used and the more
effective inhibition zone against Staph. aureus 15 mm, S. epidermidis
34 mm While two concentrations of ZnO Nanoparticles (0.6 and 1.04
mg/ml) were used and the more effective inhibition zone against S.
aureus 13 mm, Staph.epidermidis 29 mm, through disc diffusion agar
