مختصر البحث:
Carbapenemase-encoding genes have been spreading among gram-negative bacteria, which is considered the most important threats to human health. Metallo β-lactamases including IMP, VIM, and NDM are the most predominant types, which confer resistance t…
Carbapenemase-encoding genes have been spreading among gram-negative bacteria, which is considered the most important threats to human health. Metallo β-lactamases including IMP, VIM, and NDM are the most predominant types, which confer resistance to Carbapenem group. This study aimed to investigate the frequency of bla NDM , bla KPC , bla VIM , and bla IMP genes across gram-negative bacteria isolated from different clinical specimens in Medical City Hospital in Baghdad, Iraq. Fifty-two isolates were identified phenotypically using conventional biochemical tests. Vitek 2 identification system was used for confirmation of the identification. The antimicrobial sensitivity for the isolates was performed using Kirby-Bauer disk diffusion method. The carbapenemase-encoding genes (bla IMP , bla VIM , bla NDM , and bla KPC) were screened by polymerase chain reaction (PCR)-based technique. The results showed that, from a total of 52 isolates isolated from hospital in Baghdad city, 13 (25%) were Acinetobacter baumannii and 39 (75%) were Enterobacteriaceae (10 of Serratia spp, 17 of E. coli, and 12 of Enterobacter cloacae). According to antibiotic susceptibility results, 96% of isolates were resistance to ceftriaxone, 92.3% to ciprofloxacin, and 90.4% to cefotaxime by phenotypic testing. Within the isolates, bla VIM gene was the most prevalent gene, which was detected in 48.1% of the isolates, followed by bla IMP gene in 19.2%, bla NDM gene in 9.6%, and bla KPC gene in 5.7%. This study reveals that the dissemination rate of carbapenemase-encoding genes was not
