اطياف جميل ثامر التميمي

Reported in this paper responses of Juncus Aschers. (Et Buch.)Adams (Juncus A.) plants antioxidant, The catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and peroxidase activity (POD) as a scavenger of ROS for mitigating the environmental stress.

This study was conducted for determination DNA fingerprint and estimation of genetic diversity among ten wheat (Triticum aestivum L.) genotypes using seventeen RAPD Markers. Primers OPB-06, OPC-05, OPH-01 and UBC-126 gave unique fingerprint for studied genotypes. Primer OPG-09 gave higher value for polymorphism .

Growth parameters data were used in this study for the evaluation of the response on some vegetative and flowering parameters of wheat shoots (Triticum aestivum L.) Cultivar IPA 99 of growth to irrigation with magnetized water and no magnetized water, afield experiment was carried out during winter season of 2016 - 2017 at the farm of Technical institute-Kufa field, College of Science, and University of Kufa. A randomized complete block design by split plots arrangement was used with three replications. The main plot included two levels of river water and ground brackish water, while the subplot included four levels of magnetic water strength 0, 750, 1500 and 3000 GUOS.

The current study based on using morphological traits and simple sequence repeat(SSRs) markers to study variation among ten Iraqi wheat genotypes. Primers wmc596 and wmc603 produced three alleles distributed between one in wmc596 and two in wmc603 with an average number of 1.50 allele per locus . Primer wmc603 was more informative than wmc596 as produced PIC reached 0.3750. Morphological traits including whole plant ( dry weight , height ,leaf number ,leaf area and branches number) , spike( dry weight ,length and number) and weight of 100 grain used for cluster analysis

Background: DNA fingerprint ,genetic relationship were determined for ten Iraqi maize genotypes. Materials: DNA was extracted from apical fresh leaves using Genomic DNA mini Kit and ten ISSR markers were examined , amplification conducted using Thermo cycler and products examined using agarose gel electrophoresis .Results: ISSR markers amplification product ranging in their molecular size between 114bp and 1098bp producing 95 polymorphic out of 152 main band. Primers UBC808, UBC809, UBC812 , UBC814, UBC816, 844A, UBC807 and HB12 success in giving a unique fingerprint for most studied genotypes with high value of unique bands reached to five in primer UBC809. The higher value for polymorphism ,efficiency and discriminatory value were 80,0.25 and 17.89 in primers UBC808,UBC811 and UBC816 respectively while the lower value for these three parameters were 31.25 , 0.052 and 5.26 respectively produced by primer HB12. Values of genetic distance and genotypes arrangement in phyllogenetic tree in clusters and sub clusters not strongly concerned with genotypes pedigree or collection sites . Conclusion : ISSR markers could efficiently used for fingerprinting and genetic relationships, in future this would facilitate breeding programs for heterosis.

Ten of ISSRs (inter- Simple Sequence Repeats) markers were used for revealing genetic relationship, genetic diversity and DNA fingerprint of ten bread wheat (Triticumaestivum L.) genotypes. Primers varied among them in giving unique DNA fingerprint hence primers HB12, UBC811andUBC852 gave unique fingerprint for all ten studied wheat genotypes

This study was conducted for characterization of 22 tomato varieties based on total soluble seed storage protein using electrophoresis on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) .Sex varieties show distinctive fingerprint (Sanam, Helam, Oula, Kenanh, Shady lady, Carioca) and low polymorphism (46%) was observed. The overall analysis of the results reveal high level of homogeneity was detected. Reduction in the genetic diversity among modern tomato cultivars may be attributed to the recent trend towards breeding for similar plant and fruit characteristics.

This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA marker (PCR based DNA markers Simple Sequence Repeats (SSRs). To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old). The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9

This study was conducted to fingerprint of seven squash genotypes using RAPD –PCR and SDS electrophoresis techniques . RAPD profile generated high level of polymorphism. OPA-03 and OPC-19 primers showed high value for number of main, amplified and unique bands and success in giving a distinct fingerprint for all genotypes while the primer OPT-08 gave fingerprint for one genotype .The highest value for primer efficiency and discriminatory value were produced by the primers OPD-13 and OPN-06.

This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA markers (PCR based DNA markers) ; Random Amplified Polymorphic DNA (RAPDs) .To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old).The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9

This study was conducted to investigate the ability of total soluble seed protein to discriminate among 21 of maize genotypes through using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). According to SDS analysis , a total of 118 amplified bands were obtained ranging in their molecular size 18-86KDa. Five out of main nine bands were polymorphic and four monomorphic with an average of polymorphism reaches 55.5%.