Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples
AuthorsAmar M. Ali, Hussain. J. Mohammed
JournalINTERNATIONAL JOURNAL OF BIOASSAYS
Abstractnew, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood. Key word: Nickel (II) determination, Solvatochromic; 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl)-3-methyl-5-oxo-2; 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid
STUDY THE COMPLICATIONS OF ACCUMULATEDIRON IN THE TISSUE AND GLANDS IN PATIENTS WITH MAJOR β-THALASSEMIA
Authors1Basim M. Ali, A. L., 3Amar M. Ali and 4Mansoor K. Abidali *1Basim M. Ali, A. L., 2Furqan M. Auda, A. L.
JournalInternational Journal of Development Research
AbstractAbstract:
In the present study, an attempt is carried out to estimate the level of sex hormone in thalassemia major patients where. The level of these compounds will indicate the risk of tissue damage caused by oxidative stress and iron overload in thalassemia patients. Thirty five Iraqi patients with major thalassemia were participated in the present study. Their age range was 2-12 years old. In other hand Thirty five apparently healthy children were selected as control group. Serum hormones (Leutinizing hormone (LH), Follicle-stimulating hormone (FSH), Testosterone, Estradiol (E2), Prolactine (PRL) were measured using ready for use ELISA kits supplied by Monobind®, Serum levels of iron was measured spectrophotometrically. The results showed significant increase (p<0.05) in iron level in patients with thalassemia as compared with their control group, in the other hand the results showed significant decrease (p<0.05) in all sex hormone(FSH&TEST.) of thalassemic patients in comparing with healthy control group except LH&PRO. Concentration, which on significantly changing (p˃0.05) in those patients when comparing with the control group. It can be concluded that Iraqi thalassemic patients are at high risk for iron overload and iron-induced toxicities. These patients are prone to tissue injury caused by collection of iron in tissue.
