علياء فاضل سعيد ابوسعيدة

The effect of silver nanoparticles of C. spinose AgNPs on the viability of the primary protoscolices of the Echinococcus granulosus parasite was studied by means of methylthiosol tetrazolium (MTT) assay after treating with different concentrations (0.015, 0.03, 0.06, 0.12 and 0.18 μg/ mL) of silver nanoparticles of C. spinose AgNPs and compared with albendazole (0.04 g/mL) with different time periods 10 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours and 24 hours. The significant gradual decrease in the viability of protoscolices was observed with increasing concentration and time (direct relationship). The concentration was 0.18 μg/mL after 24 hours has the highest toxic effect, where the viability of protoscolex was only 10% compared to the Abendazole, where the viability of protoscolex was 49% under the same conditions.

This study included the synthesis of silver nanoparticles from the leaves of Capparies spinose plant and then studying its effectiveness IL-2 and IL-4 of E. granulosus parasite and comparing it with albendazole treatment in mice. Sixty one white mice (males) were infected with protoscolex of echinococcus granulosis , and after 30 days of infection the mice were ingested with Albendazole treatment (0.1 mL / day) and with a solution of nanoparticles at a dose of 0.1 mL / day, and after 30, 60 and 90 days after the ingesting, the mice were sacrificed for the purpose of measuring some immune markers such as IL-2, and IL-4 by using the ELISA technique in the serum of the infected mice dosed with the nanoparticles extract and compared to albendazole treatment the results showed a significant decrease after treatment compared to control.